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Kinetic Assessment and Therapeutic Modulation of Metabolic and Inflammatory Profiles in Mice on a High-Fat and Cholesterol Diet

The kinetics of metabolic and inflammatory parameters associated with obesity were evaluated in a murine diet-induced obesity (DIO) model using a diet high in fat and cholesterol. Cellular infiltration and mediator production were assessed and shown to be therapeutically modulated by the PPARgamma a...

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Autores principales: Engstrom, Laura W., Bober, Loretta, Chen, Shu-Cheng, Fine, Jay S., Li, Ying, Stanton, Michaela C., Kinsley, David, Cui, Long, Jackson, James V., Rojas-Triana, Alberto, Lundell, Daniel, Laverty, Maureen, Gustafson, Eric L., Jenh, Chung-Her, Kowalski, Timothy J., Manfra, Denise J.
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2859407/
https://www.ncbi.nlm.nih.gov/pubmed/20445733
http://dx.doi.org/10.1155/2010/970164
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author Engstrom, Laura W.
Bober, Loretta
Chen, Shu-Cheng
Fine, Jay S.
Li, Ying
Stanton, Michaela C.
Kinsley, David
Cui, Long
Jackson, James V.
Rojas-Triana, Alberto
Lundell, Daniel
Laverty, Maureen
Gustafson, Eric L.
Jenh, Chung-Her
Kowalski, Timothy J.
Manfra, Denise J.
author_facet Engstrom, Laura W.
Bober, Loretta
Chen, Shu-Cheng
Fine, Jay S.
Li, Ying
Stanton, Michaela C.
Kinsley, David
Cui, Long
Jackson, James V.
Rojas-Triana, Alberto
Lundell, Daniel
Laverty, Maureen
Gustafson, Eric L.
Jenh, Chung-Her
Kowalski, Timothy J.
Manfra, Denise J.
author_sort Engstrom, Laura W.
collection PubMed
description The kinetics of metabolic and inflammatory parameters associated with obesity were evaluated in a murine diet-induced obesity (DIO) model using a diet high in fat and cholesterol. Cellular infiltration and mediator production were assessed and shown to be therapeutically modulated by the PPARgamma agonist rosiglitazone. C57BL/6 mice were maintained on a 45% fat/ 0.12% cholesterol (HF/CH) or Chow diet for 3, 6, 16, or 27 weeks. Flow cytometry was employed to monitor peripheral blood monocytes and adipose tissue macrophages (ATM). Gene expression and protein analysis methods were used to evaluate mediator production from total epididymal fat (EF), stromal vascular fraction (SVF), and sorted SVF cells. To investigate therapeutic intervention, mice were fed a HF/CH diet for 12 weeks and then a diet formulated with rosiglitazone (5 mg/kg) for an additional 6 weeks. A HF/CH diet correlated with obesity and a dramatic proinflammatory state. Therapeutic intervention with rosiglitazone attenuated the HF/CH induced inflammation. In addition, a novel population was found that expressed the highest levels of the pro-inflammatory mediators CCL2 and IL-6.
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spelling pubmed-28594072010-05-05 Kinetic Assessment and Therapeutic Modulation of Metabolic and Inflammatory Profiles in Mice on a High-Fat and Cholesterol Diet Engstrom, Laura W. Bober, Loretta Chen, Shu-Cheng Fine, Jay S. Li, Ying Stanton, Michaela C. Kinsley, David Cui, Long Jackson, James V. Rojas-Triana, Alberto Lundell, Daniel Laverty, Maureen Gustafson, Eric L. Jenh, Chung-Her Kowalski, Timothy J. Manfra, Denise J. PPAR Res Research Article The kinetics of metabolic and inflammatory parameters associated with obesity were evaluated in a murine diet-induced obesity (DIO) model using a diet high in fat and cholesterol. Cellular infiltration and mediator production were assessed and shown to be therapeutically modulated by the PPARgamma agonist rosiglitazone. C57BL/6 mice were maintained on a 45% fat/ 0.12% cholesterol (HF/CH) or Chow diet for 3, 6, 16, or 27 weeks. Flow cytometry was employed to monitor peripheral blood monocytes and adipose tissue macrophages (ATM). Gene expression and protein analysis methods were used to evaluate mediator production from total epididymal fat (EF), stromal vascular fraction (SVF), and sorted SVF cells. To investigate therapeutic intervention, mice were fed a HF/CH diet for 12 weeks and then a diet formulated with rosiglitazone (5 mg/kg) for an additional 6 weeks. A HF/CH diet correlated with obesity and a dramatic proinflammatory state. Therapeutic intervention with rosiglitazone attenuated the HF/CH induced inflammation. In addition, a novel population was found that expressed the highest levels of the pro-inflammatory mediators CCL2 and IL-6. Hindawi Publishing Corporation 2010 2010-04-22 /pmc/articles/PMC2859407/ /pubmed/20445733 http://dx.doi.org/10.1155/2010/970164 Text en Copyright © 2010 Laura W. Engstrom et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Engstrom, Laura W.
Bober, Loretta
Chen, Shu-Cheng
Fine, Jay S.
Li, Ying
Stanton, Michaela C.
Kinsley, David
Cui, Long
Jackson, James V.
Rojas-Triana, Alberto
Lundell, Daniel
Laverty, Maureen
Gustafson, Eric L.
Jenh, Chung-Her
Kowalski, Timothy J.
Manfra, Denise J.
Kinetic Assessment and Therapeutic Modulation of Metabolic and Inflammatory Profiles in Mice on a High-Fat and Cholesterol Diet
title Kinetic Assessment and Therapeutic Modulation of Metabolic and Inflammatory Profiles in Mice on a High-Fat and Cholesterol Diet
title_full Kinetic Assessment and Therapeutic Modulation of Metabolic and Inflammatory Profiles in Mice on a High-Fat and Cholesterol Diet
title_fullStr Kinetic Assessment and Therapeutic Modulation of Metabolic and Inflammatory Profiles in Mice on a High-Fat and Cholesterol Diet
title_full_unstemmed Kinetic Assessment and Therapeutic Modulation of Metabolic and Inflammatory Profiles in Mice on a High-Fat and Cholesterol Diet
title_short Kinetic Assessment and Therapeutic Modulation of Metabolic and Inflammatory Profiles in Mice on a High-Fat and Cholesterol Diet
title_sort kinetic assessment and therapeutic modulation of metabolic and inflammatory profiles in mice on a high-fat and cholesterol diet
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2859407/
https://www.ncbi.nlm.nih.gov/pubmed/20445733
http://dx.doi.org/10.1155/2010/970164
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