CFP: a web­server for constructing sequence­based protein conformational flexibility profiles

Many proteins contain conformationally flexible segments that undergo significant changes in the backbone conformation or completely lack a well­defined conformation. Previously, we have developed the generalized local propensity (GLP), a quantitative sequence-based measure of the protein backbone flexibility. In this paper, we present the CFP (Conformational Flexibility Profile) web­server that constructs the GLP flexibility profile for a user­submitted sequence and uses this profile to identify segments with high backbone flexibility. The statistical significance of a flexible sequence segment is assessed using the discrete scan statistics based on the density of flexible residues observed in this segment. AVAILABILITY: CFP is publicly available at http://cfp.rit.albany.edu