Cargando…
Use of microarray technology to assess the time course of liver stress response after confinement exposure in gilthead sea bream (Sparus aurata L.)
BACKGROUND: Selection programs for growth and stress traits in cultured fish are fundamental to the improvement of aquaculture production. The gilthead sea bream (Sparus aurata) is the main aquacultured species in the Mediterranean area and there is considerable interest in the genetic improvement o...
Autores principales: | , , , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2010
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2860363/ https://www.ncbi.nlm.nih.gov/pubmed/20307314 http://dx.doi.org/10.1186/1471-2164-11-193 |
_version_ | 1782180575561383936 |
---|---|
author | Calduch-Giner, Josep A Davey, Grace Saera-Vila, Alfonso Houeix, Benoit Talbot, Anita Prunet, Patrick Cairns, Michael T Pérez-Sánchez, Jaume |
author_facet | Calduch-Giner, Josep A Davey, Grace Saera-Vila, Alfonso Houeix, Benoit Talbot, Anita Prunet, Patrick Cairns, Michael T Pérez-Sánchez, Jaume |
author_sort | Calduch-Giner, Josep A |
collection | PubMed |
description | BACKGROUND: Selection programs for growth and stress traits in cultured fish are fundamental to the improvement of aquaculture production. The gilthead sea bream (Sparus aurata) is the main aquacultured species in the Mediterranean area and there is considerable interest in the genetic improvement of this species. With the aim of increasing the genomic resources in gilthead sea bream and identifying genes and mechanisms underlying the physiology of the stress response, we developed a cDNA microarray for gilthead sea bream that is enriched by suppression substractive hybridization with stress and immunorelevant genes. This microarray is used to analyze the dynamics of gilthead sea bream liver expression profile after confinement exposure. RESULTS: Groups of confined and control juvenile fish were sampled at 6, 24, 72 and 120 h post exposure. GeneSpring analyses identified 202 annotated genes that appeared differentially expressed at least at one sampling time (P < 0.05). Gene expression results were validated by quantitative PCR of 10 target genes, and K-means clustering of differently expressed genes identified four major temporal gene expression profiles. Set 1 encompassed a rapid metabolic readjustment with enhanced uptake and intracellular transport of fatty acids as metabolic fuels. Set 2 was associated with a wide variety of tissue repair and remodeling processes that were mostly mediated by the stress response of the endoplasmic reticulum (ER). Sets 3 and 4 encompassed the re-establishment of cellular homeostasis with increased intracellular trafficking and scavenging of reactive oxygen species (ROS), accompanied by a bidirectional regulation of the immune system and a general decline of ROS production. CONCLUSIONS: Collectively, these findings show the complex nature of the adaptive stress response with a clear indication that the ER is an important control point for homeostatic adjustments. The study also identifies metabolic pathways which could be analyzed in greater detail to provide new insights regarding the transcriptional regulation of the stress response in fish. |
format | Text |
id | pubmed-2860363 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-28603632010-04-28 Use of microarray technology to assess the time course of liver stress response after confinement exposure in gilthead sea bream (Sparus aurata L.) Calduch-Giner, Josep A Davey, Grace Saera-Vila, Alfonso Houeix, Benoit Talbot, Anita Prunet, Patrick Cairns, Michael T Pérez-Sánchez, Jaume BMC Genomics Research Article BACKGROUND: Selection programs for growth and stress traits in cultured fish are fundamental to the improvement of aquaculture production. The gilthead sea bream (Sparus aurata) is the main aquacultured species in the Mediterranean area and there is considerable interest in the genetic improvement of this species. With the aim of increasing the genomic resources in gilthead sea bream and identifying genes and mechanisms underlying the physiology of the stress response, we developed a cDNA microarray for gilthead sea bream that is enriched by suppression substractive hybridization with stress and immunorelevant genes. This microarray is used to analyze the dynamics of gilthead sea bream liver expression profile after confinement exposure. RESULTS: Groups of confined and control juvenile fish were sampled at 6, 24, 72 and 120 h post exposure. GeneSpring analyses identified 202 annotated genes that appeared differentially expressed at least at one sampling time (P < 0.05). Gene expression results were validated by quantitative PCR of 10 target genes, and K-means clustering of differently expressed genes identified four major temporal gene expression profiles. Set 1 encompassed a rapid metabolic readjustment with enhanced uptake and intracellular transport of fatty acids as metabolic fuels. Set 2 was associated with a wide variety of tissue repair and remodeling processes that were mostly mediated by the stress response of the endoplasmic reticulum (ER). Sets 3 and 4 encompassed the re-establishment of cellular homeostasis with increased intracellular trafficking and scavenging of reactive oxygen species (ROS), accompanied by a bidirectional regulation of the immune system and a general decline of ROS production. CONCLUSIONS: Collectively, these findings show the complex nature of the adaptive stress response with a clear indication that the ER is an important control point for homeostatic adjustments. The study also identifies metabolic pathways which could be analyzed in greater detail to provide new insights regarding the transcriptional regulation of the stress response in fish. BioMed Central 2010-03-22 /pmc/articles/PMC2860363/ /pubmed/20307314 http://dx.doi.org/10.1186/1471-2164-11-193 Text en Copyright ©2010 Calduch-Giner et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Calduch-Giner, Josep A Davey, Grace Saera-Vila, Alfonso Houeix, Benoit Talbot, Anita Prunet, Patrick Cairns, Michael T Pérez-Sánchez, Jaume Use of microarray technology to assess the time course of liver stress response after confinement exposure in gilthead sea bream (Sparus aurata L.) |
title | Use of microarray technology to assess the time course of liver stress response after confinement exposure in gilthead sea bream (Sparus aurata L.) |
title_full | Use of microarray technology to assess the time course of liver stress response after confinement exposure in gilthead sea bream (Sparus aurata L.) |
title_fullStr | Use of microarray technology to assess the time course of liver stress response after confinement exposure in gilthead sea bream (Sparus aurata L.) |
title_full_unstemmed | Use of microarray technology to assess the time course of liver stress response after confinement exposure in gilthead sea bream (Sparus aurata L.) |
title_short | Use of microarray technology to assess the time course of liver stress response after confinement exposure in gilthead sea bream (Sparus aurata L.) |
title_sort | use of microarray technology to assess the time course of liver stress response after confinement exposure in gilthead sea bream (sparus aurata l.) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2860363/ https://www.ncbi.nlm.nih.gov/pubmed/20307314 http://dx.doi.org/10.1186/1471-2164-11-193 |
work_keys_str_mv | AT calduchginerjosepa useofmicroarraytechnologytoassessthetimecourseofliverstressresponseafterconfinementexposureingiltheadseabreamsparusauratal AT daveygrace useofmicroarraytechnologytoassessthetimecourseofliverstressresponseafterconfinementexposureingiltheadseabreamsparusauratal AT saeravilaalfonso useofmicroarraytechnologytoassessthetimecourseofliverstressresponseafterconfinementexposureingiltheadseabreamsparusauratal AT houeixbenoit useofmicroarraytechnologytoassessthetimecourseofliverstressresponseafterconfinementexposureingiltheadseabreamsparusauratal AT talbotanita useofmicroarraytechnologytoassessthetimecourseofliverstressresponseafterconfinementexposureingiltheadseabreamsparusauratal AT prunetpatrick useofmicroarraytechnologytoassessthetimecourseofliverstressresponseafterconfinementexposureingiltheadseabreamsparusauratal AT cairnsmichaelt useofmicroarraytechnologytoassessthetimecourseofliverstressresponseafterconfinementexposureingiltheadseabreamsparusauratal AT perezsanchezjaume useofmicroarraytechnologytoassessthetimecourseofliverstressresponseafterconfinementexposureingiltheadseabreamsparusauratal |