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A quantitative proteomic analysis of long-term memory

BACKGROUND: Memory is the ability to store, retain, and later retrieve learned information. Long-term memory (LTM) formation requires: DNA transcription, RNA translation, and the trafficking of newly synthesized proteins. Several components of these processes have already been identified. However, d...

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Detalles Bibliográficos
Autores principales: Rosenegger, David, Wright, Cynthia, Lukowiak, Ken
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2860487/
https://www.ncbi.nlm.nih.gov/pubmed/20331892
http://dx.doi.org/10.1186/1756-6606-3-9
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author Rosenegger, David
Wright, Cynthia
Lukowiak, Ken
author_facet Rosenegger, David
Wright, Cynthia
Lukowiak, Ken
author_sort Rosenegger, David
collection PubMed
description BACKGROUND: Memory is the ability to store, retain, and later retrieve learned information. Long-term memory (LTM) formation requires: DNA transcription, RNA translation, and the trafficking of newly synthesized proteins. Several components of these processes have already been identified. However, due to the complexity of the memory formation process, there likely remain many yet to be identified proteins involved in memory formation and persistence. RESULTS: Here we use a quantitative proteomic method to identify novel memory-associated proteins in neural tissue taken from animals that were trained in vivo to form a long-term memory. We identified 8 proteins that were significantly up-regulated, and 13 that were significantly down-regulated in the LTM trained animals as compared to two different control groups. In addition we found 19 proteins unique to the trained animals, and 12 unique proteins found only in the control animals. CONCLUSIONS: These results both confirm the involvement of previously identified memory proteins such as: protein kinase C (PKC), adenylate cyclase (AC), and proteins in the mitogen-activated protein kinase (MAPK) pathway. In addition these results provide novel protein candidates (e.g. UHRF1 binding protein) on which to base future studies.
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spelling pubmed-28604872010-04-28 A quantitative proteomic analysis of long-term memory Rosenegger, David Wright, Cynthia Lukowiak, Ken Mol Brain Research BACKGROUND: Memory is the ability to store, retain, and later retrieve learned information. Long-term memory (LTM) formation requires: DNA transcription, RNA translation, and the trafficking of newly synthesized proteins. Several components of these processes have already been identified. However, due to the complexity of the memory formation process, there likely remain many yet to be identified proteins involved in memory formation and persistence. RESULTS: Here we use a quantitative proteomic method to identify novel memory-associated proteins in neural tissue taken from animals that were trained in vivo to form a long-term memory. We identified 8 proteins that were significantly up-regulated, and 13 that were significantly down-regulated in the LTM trained animals as compared to two different control groups. In addition we found 19 proteins unique to the trained animals, and 12 unique proteins found only in the control animals. CONCLUSIONS: These results both confirm the involvement of previously identified memory proteins such as: protein kinase C (PKC), adenylate cyclase (AC), and proteins in the mitogen-activated protein kinase (MAPK) pathway. In addition these results provide novel protein candidates (e.g. UHRF1 binding protein) on which to base future studies. BioMed Central 2010-03-23 /pmc/articles/PMC2860487/ /pubmed/20331892 http://dx.doi.org/10.1186/1756-6606-3-9 Text en Copyright ©2010 Rosenegger et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Rosenegger, David
Wright, Cynthia
Lukowiak, Ken
A quantitative proteomic analysis of long-term memory
title A quantitative proteomic analysis of long-term memory
title_full A quantitative proteomic analysis of long-term memory
title_fullStr A quantitative proteomic analysis of long-term memory
title_full_unstemmed A quantitative proteomic analysis of long-term memory
title_short A quantitative proteomic analysis of long-term memory
title_sort quantitative proteomic analysis of long-term memory
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2860487/
https://www.ncbi.nlm.nih.gov/pubmed/20331892
http://dx.doi.org/10.1186/1756-6606-3-9
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