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Persistent Synapse Loss Induced by Repetitive LTD in Developing Rat Hippocampal Neurons
Synaptic pruning is a physiological event that eliminates excessive or inappropriate synapses to form proper synaptic connections during development of neurons. Appropriate synaptic pruning is required for normal neural development. However, the mechanism of synaptic pruning is not fully understood....
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Formato: | Texto |
Lenguaje: | English |
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Public Library of Science
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2861005/ https://www.ncbi.nlm.nih.gov/pubmed/20436928 http://dx.doi.org/10.1371/journal.pone.0010390 |
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author | Shinoda, Yo Tanaka, Tsunehiro Tominaga-Yoshino, Keiko Ogura, Akihiko |
author_facet | Shinoda, Yo Tanaka, Tsunehiro Tominaga-Yoshino, Keiko Ogura, Akihiko |
author_sort | Shinoda, Yo |
collection | PubMed |
description | Synaptic pruning is a physiological event that eliminates excessive or inappropriate synapses to form proper synaptic connections during development of neurons. Appropriate synaptic pruning is required for normal neural development. However, the mechanism of synaptic pruning is not fully understood. Strength of synaptic activity under competitive circumstances is thought to act as a selective force for synaptic pruning. Long-term depression (LTD) is a synaptic plasticity showing persistent decreased synaptic efficacy, which is accompanied by morphological changes of dendritic spines including transient retraction. Repetitive induction of LTD has been shown to cause persistent loss of synapses in mature neurons. Here, we show that multiple, but not single, induction of LTD caused a persistent reduction in the number of dendritic synapses in cultured rat developing hippocampal neurons. When LTD was induced in 14 days in vitro cultures by application of (RS)-3,5-dihydroxyphenylglycine (DHPG), a group I metabotropic glutamate receptor (mGluR) agonist, and repeated three times with a one day interval, there was a significant decrease in the number of dendritic synapses. This effect continued up to at least two weeks after the triple LTD induction. The persistent reduction in synapse number occurred in the proximal dendrites, but not the distal dendrites, and was prevented by simultaneous application of the group I/II mGluR antagonist (S)-a-methyl-4-carboxyphenylglycine (MCPG). In conclusion, we found that repetitive LTD induction in developing neurons elicits synaptic pruning and contributes to activity-dependent regulation of synapse number in rat hippocampal neurons. |
format | Text |
id | pubmed-2861005 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-28610052010-04-30 Persistent Synapse Loss Induced by Repetitive LTD in Developing Rat Hippocampal Neurons Shinoda, Yo Tanaka, Tsunehiro Tominaga-Yoshino, Keiko Ogura, Akihiko PLoS One Research Article Synaptic pruning is a physiological event that eliminates excessive or inappropriate synapses to form proper synaptic connections during development of neurons. Appropriate synaptic pruning is required for normal neural development. However, the mechanism of synaptic pruning is not fully understood. Strength of synaptic activity under competitive circumstances is thought to act as a selective force for synaptic pruning. Long-term depression (LTD) is a synaptic plasticity showing persistent decreased synaptic efficacy, which is accompanied by morphological changes of dendritic spines including transient retraction. Repetitive induction of LTD has been shown to cause persistent loss of synapses in mature neurons. Here, we show that multiple, but not single, induction of LTD caused a persistent reduction in the number of dendritic synapses in cultured rat developing hippocampal neurons. When LTD was induced in 14 days in vitro cultures by application of (RS)-3,5-dihydroxyphenylglycine (DHPG), a group I metabotropic glutamate receptor (mGluR) agonist, and repeated three times with a one day interval, there was a significant decrease in the number of dendritic synapses. This effect continued up to at least two weeks after the triple LTD induction. The persistent reduction in synapse number occurred in the proximal dendrites, but not the distal dendrites, and was prevented by simultaneous application of the group I/II mGluR antagonist (S)-a-methyl-4-carboxyphenylglycine (MCPG). In conclusion, we found that repetitive LTD induction in developing neurons elicits synaptic pruning and contributes to activity-dependent regulation of synapse number in rat hippocampal neurons. Public Library of Science 2010-04-28 /pmc/articles/PMC2861005/ /pubmed/20436928 http://dx.doi.org/10.1371/journal.pone.0010390 Text en Shinoda et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Shinoda, Yo Tanaka, Tsunehiro Tominaga-Yoshino, Keiko Ogura, Akihiko Persistent Synapse Loss Induced by Repetitive LTD in Developing Rat Hippocampal Neurons |
title | Persistent Synapse Loss Induced by Repetitive LTD in Developing Rat Hippocampal Neurons |
title_full | Persistent Synapse Loss Induced by Repetitive LTD in Developing Rat Hippocampal Neurons |
title_fullStr | Persistent Synapse Loss Induced by Repetitive LTD in Developing Rat Hippocampal Neurons |
title_full_unstemmed | Persistent Synapse Loss Induced by Repetitive LTD in Developing Rat Hippocampal Neurons |
title_short | Persistent Synapse Loss Induced by Repetitive LTD in Developing Rat Hippocampal Neurons |
title_sort | persistent synapse loss induced by repetitive ltd in developing rat hippocampal neurons |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2861005/ https://www.ncbi.nlm.nih.gov/pubmed/20436928 http://dx.doi.org/10.1371/journal.pone.0010390 |
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