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Formalin-Induced Fluorescence Reveals Cell Shape and Morphology in Biological Tissue Samples
Ultramicroscopy is a powerful tool to reveal detailed three-dimensional structures of large microscopical objects. Using high magnification, we observed that formalin induces fluorescence more in extra-cellular space and stains cellular structures negatively, rendering cells as dark objects in front...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2861007/ https://www.ncbi.nlm.nih.gov/pubmed/20436930 http://dx.doi.org/10.1371/journal.pone.0010391 |
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author | Leischner, Ulrich Schierloh, Anja Zieglgänsberger, Walter Dodt, Hans-Ulrich |
author_facet | Leischner, Ulrich Schierloh, Anja Zieglgänsberger, Walter Dodt, Hans-Ulrich |
author_sort | Leischner, Ulrich |
collection | PubMed |
description | Ultramicroscopy is a powerful tool to reveal detailed three-dimensional structures of large microscopical objects. Using high magnification, we observed that formalin induces fluorescence more in extra-cellular space and stains cellular structures negatively, rendering cells as dark objects in front of a bright background. Here, we show this effect on a three-dimensional image stack of a hippocampus sample, focusing on the CA1 region. This method, called FIF-Ultramicroscopy, allows for the three-dimensional observation of cellular structures in various tissue types without complicated staining techniques. |
format | Text |
id | pubmed-2861007 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-28610072010-04-30 Formalin-Induced Fluorescence Reveals Cell Shape and Morphology in Biological Tissue Samples Leischner, Ulrich Schierloh, Anja Zieglgänsberger, Walter Dodt, Hans-Ulrich PLoS One Research Article Ultramicroscopy is a powerful tool to reveal detailed three-dimensional structures of large microscopical objects. Using high magnification, we observed that formalin induces fluorescence more in extra-cellular space and stains cellular structures negatively, rendering cells as dark objects in front of a bright background. Here, we show this effect on a three-dimensional image stack of a hippocampus sample, focusing on the CA1 region. This method, called FIF-Ultramicroscopy, allows for the three-dimensional observation of cellular structures in various tissue types without complicated staining techniques. Public Library of Science 2010-04-28 /pmc/articles/PMC2861007/ /pubmed/20436930 http://dx.doi.org/10.1371/journal.pone.0010391 Text en Leischner et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Leischner, Ulrich Schierloh, Anja Zieglgänsberger, Walter Dodt, Hans-Ulrich Formalin-Induced Fluorescence Reveals Cell Shape and Morphology in Biological Tissue Samples |
title | Formalin-Induced Fluorescence Reveals Cell Shape and Morphology in Biological Tissue Samples |
title_full | Formalin-Induced Fluorescence Reveals Cell Shape and Morphology in Biological Tissue Samples |
title_fullStr | Formalin-Induced Fluorescence Reveals Cell Shape and Morphology in Biological Tissue Samples |
title_full_unstemmed | Formalin-Induced Fluorescence Reveals Cell Shape and Morphology in Biological Tissue Samples |
title_short | Formalin-Induced Fluorescence Reveals Cell Shape and Morphology in Biological Tissue Samples |
title_sort | formalin-induced fluorescence reveals cell shape and morphology in biological tissue samples |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2861007/ https://www.ncbi.nlm.nih.gov/pubmed/20436930 http://dx.doi.org/10.1371/journal.pone.0010391 |
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