Formalin-Induced Fluorescence Reveals Cell Shape and Morphology in Biological Tissue Samples

Ultramicroscopy is a powerful tool to reveal detailed three-dimensional structures of large microscopical objects. Using high magnification, we observed that formalin induces fluorescence more in extra-cellular space and stains cellular structures negatively, rendering cells as dark objects in front...

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Autores principales: Leischner, Ulrich, Schierloh, Anja, Zieglgänsberger, Walter, Dodt, Hans-Ulrich
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2861007/
https://www.ncbi.nlm.nih.gov/pubmed/20436930
http://dx.doi.org/10.1371/journal.pone.0010391
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author Leischner, Ulrich
Schierloh, Anja
Zieglgänsberger, Walter
Dodt, Hans-Ulrich
author_facet Leischner, Ulrich
Schierloh, Anja
Zieglgänsberger, Walter
Dodt, Hans-Ulrich
author_sort Leischner, Ulrich
collection PubMed
description Ultramicroscopy is a powerful tool to reveal detailed three-dimensional structures of large microscopical objects. Using high magnification, we observed that formalin induces fluorescence more in extra-cellular space and stains cellular structures negatively, rendering cells as dark objects in front of a bright background. Here, we show this effect on a three-dimensional image stack of a hippocampus sample, focusing on the CA1 region. This method, called FIF-Ultramicroscopy, allows for the three-dimensional observation of cellular structures in various tissue types without complicated staining techniques.
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spelling pubmed-28610072010-04-30 Formalin-Induced Fluorescence Reveals Cell Shape and Morphology in Biological Tissue Samples Leischner, Ulrich Schierloh, Anja Zieglgänsberger, Walter Dodt, Hans-Ulrich PLoS One Research Article Ultramicroscopy is a powerful tool to reveal detailed three-dimensional structures of large microscopical objects. Using high magnification, we observed that formalin induces fluorescence more in extra-cellular space and stains cellular structures negatively, rendering cells as dark objects in front of a bright background. Here, we show this effect on a three-dimensional image stack of a hippocampus sample, focusing on the CA1 region. This method, called FIF-Ultramicroscopy, allows for the three-dimensional observation of cellular structures in various tissue types without complicated staining techniques. Public Library of Science 2010-04-28 /pmc/articles/PMC2861007/ /pubmed/20436930 http://dx.doi.org/10.1371/journal.pone.0010391 Text en Leischner et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Leischner, Ulrich
Schierloh, Anja
Zieglgänsberger, Walter
Dodt, Hans-Ulrich
Formalin-Induced Fluorescence Reveals Cell Shape and Morphology in Biological Tissue Samples
title Formalin-Induced Fluorescence Reveals Cell Shape and Morphology in Biological Tissue Samples
title_full Formalin-Induced Fluorescence Reveals Cell Shape and Morphology in Biological Tissue Samples
title_fullStr Formalin-Induced Fluorescence Reveals Cell Shape and Morphology in Biological Tissue Samples
title_full_unstemmed Formalin-Induced Fluorescence Reveals Cell Shape and Morphology in Biological Tissue Samples
title_short Formalin-Induced Fluorescence Reveals Cell Shape and Morphology in Biological Tissue Samples
title_sort formalin-induced fluorescence reveals cell shape and morphology in biological tissue samples
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2861007/
https://www.ncbi.nlm.nih.gov/pubmed/20436930
http://dx.doi.org/10.1371/journal.pone.0010391
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AT schierlohanja formalininducedfluorescencerevealscellshapeandmorphologyinbiologicaltissuesamples
AT zieglgansbergerwalter formalininducedfluorescencerevealscellshapeandmorphologyinbiologicaltissuesamples
AT dodthansulrich formalininducedfluorescencerevealscellshapeandmorphologyinbiologicaltissuesamples

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