Cargando…

Analysis of Human Small Nucleolar RNAs (snoRNA) and the Development of snoRNA Modulator of Gene Expression Vectors

Human small nucleolar RNAs (snoRNAs) that copurify with nucleoli isolated from HeLa cells have been characterized. Novel fibrillarin-associated snoRNAs were detected that allowed the creation of a new vector system for the targeted knockdown of one or more genes in mammalian cells. The snoMEN (snoRN...

Descripción completa

Detalles Bibliográficos
Autores principales: Ono, Motoharu, Yamada, Kayo, Avolio, Fabio, Scott, Michelle S., van Koningsbruggen, Silvana, Barton, Geoffrey J., Lamond, Angus I.
Formato: Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2861615/
https://www.ncbi.nlm.nih.gov/pubmed/20219969
http://dx.doi.org/10.1091/mbc.E10-01-0078
_version_ 1782180646503841792
author Ono, Motoharu
Yamada, Kayo
Avolio, Fabio
Scott, Michelle S.
van Koningsbruggen, Silvana
Barton, Geoffrey J.
Lamond, Angus I.
author_facet Ono, Motoharu
Yamada, Kayo
Avolio, Fabio
Scott, Michelle S.
van Koningsbruggen, Silvana
Barton, Geoffrey J.
Lamond, Angus I.
author_sort Ono, Motoharu
collection PubMed
description Human small nucleolar RNAs (snoRNAs) that copurify with nucleoli isolated from HeLa cells have been characterized. Novel fibrillarin-associated snoRNAs were detected that allowed the creation of a new vector system for the targeted knockdown of one or more genes in mammalian cells. The snoMEN (snoRNA modulator of gene expressioN) vector technology is based on snoRNA HBII-180C, which contains an internal sequence that can be manipulated to make it complementary to RNA targets. Gene-specific knockdowns are demonstrated for endogenous cellular proteins and for G/YFP-fusion proteins. Multiplex snoMEN vectors coexpress multiple snoRNAs in one transcript, targeted either to different genes or to different sites in the same gene. Protein replacement snoMEN vectors can express a single transcript combining cDNA for a tagged protein with introns containing cognate snoRNAs targeted to knockdown the endogenous cellular protein. We foresee applications for snoMEN vectors in basic gene expression research, target validation, and gene therapy.
format Text
id pubmed-2861615
institution National Center for Biotechnology Information
language English
publishDate 2010
publisher The American Society for Cell Biology
record_format MEDLINE/PubMed
spelling pubmed-28616152010-07-16 Analysis of Human Small Nucleolar RNAs (snoRNA) and the Development of snoRNA Modulator of Gene Expression Vectors Ono, Motoharu Yamada, Kayo Avolio, Fabio Scott, Michelle S. van Koningsbruggen, Silvana Barton, Geoffrey J. Lamond, Angus I. Mol Biol Cell Articles Human small nucleolar RNAs (snoRNAs) that copurify with nucleoli isolated from HeLa cells have been characterized. Novel fibrillarin-associated snoRNAs were detected that allowed the creation of a new vector system for the targeted knockdown of one or more genes in mammalian cells. The snoMEN (snoRNA modulator of gene expressioN) vector technology is based on snoRNA HBII-180C, which contains an internal sequence that can be manipulated to make it complementary to RNA targets. Gene-specific knockdowns are demonstrated for endogenous cellular proteins and for G/YFP-fusion proteins. Multiplex snoMEN vectors coexpress multiple snoRNAs in one transcript, targeted either to different genes or to different sites in the same gene. Protein replacement snoMEN vectors can express a single transcript combining cDNA for a tagged protein with introns containing cognate snoRNAs targeted to knockdown the endogenous cellular protein. We foresee applications for snoMEN vectors in basic gene expression research, target validation, and gene therapy. The American Society for Cell Biology 2010-05-01 /pmc/articles/PMC2861615/ /pubmed/20219969 http://dx.doi.org/10.1091/mbc.E10-01-0078 Text en © 2010 by The American Society for Cell Biology
spellingShingle Articles
Ono, Motoharu
Yamada, Kayo
Avolio, Fabio
Scott, Michelle S.
van Koningsbruggen, Silvana
Barton, Geoffrey J.
Lamond, Angus I.
Analysis of Human Small Nucleolar RNAs (snoRNA) and the Development of snoRNA Modulator of Gene Expression Vectors
title Analysis of Human Small Nucleolar RNAs (snoRNA) and the Development of snoRNA Modulator of Gene Expression Vectors
title_full Analysis of Human Small Nucleolar RNAs (snoRNA) and the Development of snoRNA Modulator of Gene Expression Vectors
title_fullStr Analysis of Human Small Nucleolar RNAs (snoRNA) and the Development of snoRNA Modulator of Gene Expression Vectors
title_full_unstemmed Analysis of Human Small Nucleolar RNAs (snoRNA) and the Development of snoRNA Modulator of Gene Expression Vectors
title_short Analysis of Human Small Nucleolar RNAs (snoRNA) and the Development of snoRNA Modulator of Gene Expression Vectors
title_sort analysis of human small nucleolar rnas (snorna) and the development of snorna modulator of gene expression vectors
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2861615/
https://www.ncbi.nlm.nih.gov/pubmed/20219969
http://dx.doi.org/10.1091/mbc.E10-01-0078
work_keys_str_mv AT onomotoharu analysisofhumansmallnucleolarrnassnornaandthedevelopmentofsnornamodulatorofgeneexpressionvectors
AT yamadakayo analysisofhumansmallnucleolarrnassnornaandthedevelopmentofsnornamodulatorofgeneexpressionvectors
AT avoliofabio analysisofhumansmallnucleolarrnassnornaandthedevelopmentofsnornamodulatorofgeneexpressionvectors
AT scottmichelles analysisofhumansmallnucleolarrnassnornaandthedevelopmentofsnornamodulatorofgeneexpressionvectors
AT vankoningsbruggensilvana analysisofhumansmallnucleolarrnassnornaandthedevelopmentofsnornamodulatorofgeneexpressionvectors
AT bartongeoffreyj analysisofhumansmallnucleolarrnassnornaandthedevelopmentofsnornamodulatorofgeneexpressionvectors
AT lamondangusi analysisofhumansmallnucleolarrnassnornaandthedevelopmentofsnornamodulatorofgeneexpressionvectors