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Analysis of Human Small Nucleolar RNAs (snoRNA) and the Development of snoRNA Modulator of Gene Expression Vectors
Human small nucleolar RNAs (snoRNAs) that copurify with nucleoli isolated from HeLa cells have been characterized. Novel fibrillarin-associated snoRNAs were detected that allowed the creation of a new vector system for the targeted knockdown of one or more genes in mammalian cells. The snoMEN (snoRN...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2861615/ https://www.ncbi.nlm.nih.gov/pubmed/20219969 http://dx.doi.org/10.1091/mbc.E10-01-0078 |
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author | Ono, Motoharu Yamada, Kayo Avolio, Fabio Scott, Michelle S. van Koningsbruggen, Silvana Barton, Geoffrey J. Lamond, Angus I. |
author_facet | Ono, Motoharu Yamada, Kayo Avolio, Fabio Scott, Michelle S. van Koningsbruggen, Silvana Barton, Geoffrey J. Lamond, Angus I. |
author_sort | Ono, Motoharu |
collection | PubMed |
description | Human small nucleolar RNAs (snoRNAs) that copurify with nucleoli isolated from HeLa cells have been characterized. Novel fibrillarin-associated snoRNAs were detected that allowed the creation of a new vector system for the targeted knockdown of one or more genes in mammalian cells. The snoMEN (snoRNA modulator of gene expressioN) vector technology is based on snoRNA HBII-180C, which contains an internal sequence that can be manipulated to make it complementary to RNA targets. Gene-specific knockdowns are demonstrated for endogenous cellular proteins and for G/YFP-fusion proteins. Multiplex snoMEN vectors coexpress multiple snoRNAs in one transcript, targeted either to different genes or to different sites in the same gene. Protein replacement snoMEN vectors can express a single transcript combining cDNA for a tagged protein with introns containing cognate snoRNAs targeted to knockdown the endogenous cellular protein. We foresee applications for snoMEN vectors in basic gene expression research, target validation, and gene therapy. |
format | Text |
id | pubmed-2861615 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-28616152010-07-16 Analysis of Human Small Nucleolar RNAs (snoRNA) and the Development of snoRNA Modulator of Gene Expression Vectors Ono, Motoharu Yamada, Kayo Avolio, Fabio Scott, Michelle S. van Koningsbruggen, Silvana Barton, Geoffrey J. Lamond, Angus I. Mol Biol Cell Articles Human small nucleolar RNAs (snoRNAs) that copurify with nucleoli isolated from HeLa cells have been characterized. Novel fibrillarin-associated snoRNAs were detected that allowed the creation of a new vector system for the targeted knockdown of one or more genes in mammalian cells. The snoMEN (snoRNA modulator of gene expressioN) vector technology is based on snoRNA HBII-180C, which contains an internal sequence that can be manipulated to make it complementary to RNA targets. Gene-specific knockdowns are demonstrated for endogenous cellular proteins and for G/YFP-fusion proteins. Multiplex snoMEN vectors coexpress multiple snoRNAs in one transcript, targeted either to different genes or to different sites in the same gene. Protein replacement snoMEN vectors can express a single transcript combining cDNA for a tagged protein with introns containing cognate snoRNAs targeted to knockdown the endogenous cellular protein. We foresee applications for snoMEN vectors in basic gene expression research, target validation, and gene therapy. The American Society for Cell Biology 2010-05-01 /pmc/articles/PMC2861615/ /pubmed/20219969 http://dx.doi.org/10.1091/mbc.E10-01-0078 Text en © 2010 by The American Society for Cell Biology |
spellingShingle | Articles Ono, Motoharu Yamada, Kayo Avolio, Fabio Scott, Michelle S. van Koningsbruggen, Silvana Barton, Geoffrey J. Lamond, Angus I. Analysis of Human Small Nucleolar RNAs (snoRNA) and the Development of snoRNA Modulator of Gene Expression Vectors |
title | Analysis of Human Small Nucleolar RNAs (snoRNA) and the Development of snoRNA Modulator of Gene Expression Vectors |
title_full | Analysis of Human Small Nucleolar RNAs (snoRNA) and the Development of snoRNA Modulator of Gene Expression Vectors |
title_fullStr | Analysis of Human Small Nucleolar RNAs (snoRNA) and the Development of snoRNA Modulator of Gene Expression Vectors |
title_full_unstemmed | Analysis of Human Small Nucleolar RNAs (snoRNA) and the Development of snoRNA Modulator of Gene Expression Vectors |
title_short | Analysis of Human Small Nucleolar RNAs (snoRNA) and the Development of snoRNA Modulator of Gene Expression Vectors |
title_sort | analysis of human small nucleolar rnas (snorna) and the development of snorna modulator of gene expression vectors |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2861615/ https://www.ncbi.nlm.nih.gov/pubmed/20219969 http://dx.doi.org/10.1091/mbc.E10-01-0078 |
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