FRT-seq: Amplification-free, strand-specific, transcriptome sequencing

We report an alternative approach to transcriptome sequencing for the Illumina Genome Analyzer, in which the reverse transcription reaction takes place on the flowcell. No amplification is performed during the library preparation, so PCR biases and duplicates are avoided. Since the template is poly...

Descripción completa

Detalles Bibliográficos
Autores principales: Mamanova, Lira, Andrews, Robert M., James, Keith D., Sheridan, Elizabeth M., Ellis, Peter D., Langford, Cordelia F., Ost, Tobias W.B., Collins, John E., Turner, Daniel J.
Formato: Texto
Lenguaje:English
Publicado: 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2861772/
https://www.ncbi.nlm.nih.gov/pubmed/20081834
http://dx.doi.org/10.1038/nmeth.1417
_version_ 1782180673766817792
author Mamanova, Lira
Andrews, Robert M.
James, Keith D.
Sheridan, Elizabeth M.
Ellis, Peter D.
Langford, Cordelia F.
Ost, Tobias W.B.
Collins, John E.
Turner, Daniel J.
author_facet Mamanova, Lira
Andrews, Robert M.
James, Keith D.
Sheridan, Elizabeth M.
Ellis, Peter D.
Langford, Cordelia F.
Ost, Tobias W.B.
Collins, John E.
Turner, Daniel J.
author_sort Mamanova, Lira
collection PubMed
description We report an alternative approach to transcriptome sequencing for the Illumina Genome Analyzer, in which the reverse transcription reaction takes place on the flowcell. No amplification is performed during the library preparation, so PCR biases and duplicates are avoided. Since the template is poly A(+) RNA rather than cDNA, the resulting sequences are necessarily strand-specific. The method is compatible with paired- or single-ended sequencing.
format Text
id pubmed-2861772
institution National Center for Biotechnology Information
language English
publishDate 2010
record_format MEDLINE/PubMed
spelling pubmed-28617722010-08-01 FRT-seq: Amplification-free, strand-specific, transcriptome sequencing Mamanova, Lira Andrews, Robert M. James, Keith D. Sheridan, Elizabeth M. Ellis, Peter D. Langford, Cordelia F. Ost, Tobias W.B. Collins, John E. Turner, Daniel J. Nat Methods Article We report an alternative approach to transcriptome sequencing for the Illumina Genome Analyzer, in which the reverse transcription reaction takes place on the flowcell. No amplification is performed during the library preparation, so PCR biases and duplicates are avoided. Since the template is poly A(+) RNA rather than cDNA, the resulting sequences are necessarily strand-specific. The method is compatible with paired- or single-ended sequencing. 2010-01-17 2010-02 /pmc/articles/PMC2861772/ /pubmed/20081834 http://dx.doi.org/10.1038/nmeth.1417 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Mamanova, Lira
Andrews, Robert M.
James, Keith D.
Sheridan, Elizabeth M.
Ellis, Peter D.
Langford, Cordelia F.
Ost, Tobias W.B.
Collins, John E.
Turner, Daniel J.
FRT-seq: Amplification-free, strand-specific, transcriptome sequencing
title FRT-seq: Amplification-free, strand-specific, transcriptome sequencing
title_full FRT-seq: Amplification-free, strand-specific, transcriptome sequencing
title_fullStr FRT-seq: Amplification-free, strand-specific, transcriptome sequencing
title_full_unstemmed FRT-seq: Amplification-free, strand-specific, transcriptome sequencing
title_short FRT-seq: Amplification-free, strand-specific, transcriptome sequencing
title_sort frt-seq: amplification-free, strand-specific, transcriptome sequencing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2861772/
https://www.ncbi.nlm.nih.gov/pubmed/20081834
http://dx.doi.org/10.1038/nmeth.1417
work_keys_str_mv AT mamanovalira frtseqamplificationfreestrandspecifictranscriptomesequencing
AT andrewsrobertm frtseqamplificationfreestrandspecifictranscriptomesequencing
AT jameskeithd frtseqamplificationfreestrandspecifictranscriptomesequencing
AT sheridanelizabethm frtseqamplificationfreestrandspecifictranscriptomesequencing
AT ellispeterd frtseqamplificationfreestrandspecifictranscriptomesequencing
AT langfordcordeliaf frtseqamplificationfreestrandspecifictranscriptomesequencing
AT osttobiaswb frtseqamplificationfreestrandspecifictranscriptomesequencing
AT collinsjohne frtseqamplificationfreestrandspecifictranscriptomesequencing
AT turnerdanielj frtseqamplificationfreestrandspecifictranscriptomesequencing

Ejemplares similares