FRT-seq: Amplification-free, strand-specific, transcriptome sequencing

We report an alternative approach to transcriptome sequencing for the Illumina Genome Analyzer, in which the reverse transcription reaction takes place on the flowcell. No amplification is performed during the library preparation, so PCR biases and duplicates are avoided. Since the template is poly...

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Detalles Bibliográficos
Autores principales: Mamanova, Lira, Andrews, Robert M., James, Keith D., Sheridan, Elizabeth M., Ellis, Peter D., Langford, Cordelia F., Ost, Tobias W.B., Collins, John E., Turner, Daniel J.
Formato: Texto
Lenguaje:English
Publicado: 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2861772/
https://www.ncbi.nlm.nih.gov/pubmed/20081834
http://dx.doi.org/10.1038/nmeth.1417

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2861772/
https://www.ncbi.nlm.nih.gov/pubmed/20081834
http://dx.doi.org/10.1038/nmeth.1417

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