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Differential expression of proteomics models of colorectal cancer, colorectal benign disease and healthy controls
BACKGROUND: Colorectal cancer (CRC) is often diagnosed at a late stage with concomitant poor prognosis. The hypersensitive analytical technique of proteomics can detect molecular changes before the tumor is palpable. The surface-enhanced laser desorption/ionization-time of flight-mass spectra (SELDI...
Autores principales: | , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2862023/ https://www.ncbi.nlm.nih.gov/pubmed/20334691 http://dx.doi.org/10.1186/1477-5956-8-16 |
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author | Liu, Ming Li, Chun-Feng Chen, Hong-Sheng Lin, Luo-Qiang Zhang, Chun-Peng Zhao, Jin-Lu Liu, Yan Zhang, Shu-Jun Jin, Jun-Chao Wang, Lei Liu, Jia-Ren |
author_facet | Liu, Ming Li, Chun-Feng Chen, Hong-Sheng Lin, Luo-Qiang Zhang, Chun-Peng Zhao, Jin-Lu Liu, Yan Zhang, Shu-Jun Jin, Jun-Chao Wang, Lei Liu, Jia-Ren |
author_sort | Liu, Ming |
collection | PubMed |
description | BACKGROUND: Colorectal cancer (CRC) is often diagnosed at a late stage with concomitant poor prognosis. The hypersensitive analytical technique of proteomics can detect molecular changes before the tumor is palpable. The surface-enhanced laser desorption/ionization-time of flight-mass spectra (SELDI-TOF-MS) is a newly-developed technique of evaluating protein separation in recent years. The protein chips have established the expression of tumor protein in the serum specimens and become the newly discovered markers for tumor diagnosis. The objective of this study was to find new markers of the diagnosis among groups of CRC, colorectal benign diseases (CBD) and healthy controls. The assay of SELDI-TOF-MS with analytical technique of protein-chip bioinformatics was used to detect the expression of protein mass peaks in the sera of patients or controls. One hundred serum samples, including 52 cases of colorectal cancer, 27 cases of colorectal benign disease, and 21 cases of healthy controls, were examined by SELDI-TOF-MS with WCX2 protein-chips. RESULTS: The diagnostic models (I, II and III) were setup by analyzed the data and sieved markers using Ciphergen - Protein-Chip-Software 5.1. These models were combined with 3 protein mass peaks to discriminate CRC, CBD, and healthy controls. The accuracy, the sensitivity and the particularity of cross verification of these models are all highly over 80%. CONCLUSIONS: The SELDI-TOF-MS is a useful tool to help diagnose colorectal cancer, especially during the early stage. However, identification of the significantly differentiated proteins needs further study. |
format | Text |
id | pubmed-2862023 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-28620232010-05-01 Differential expression of proteomics models of colorectal cancer, colorectal benign disease and healthy controls Liu, Ming Li, Chun-Feng Chen, Hong-Sheng Lin, Luo-Qiang Zhang, Chun-Peng Zhao, Jin-Lu Liu, Yan Zhang, Shu-Jun Jin, Jun-Chao Wang, Lei Liu, Jia-Ren Proteome Sci Research BACKGROUND: Colorectal cancer (CRC) is often diagnosed at a late stage with concomitant poor prognosis. The hypersensitive analytical technique of proteomics can detect molecular changes before the tumor is palpable. The surface-enhanced laser desorption/ionization-time of flight-mass spectra (SELDI-TOF-MS) is a newly-developed technique of evaluating protein separation in recent years. The protein chips have established the expression of tumor protein in the serum specimens and become the newly discovered markers for tumor diagnosis. The objective of this study was to find new markers of the diagnosis among groups of CRC, colorectal benign diseases (CBD) and healthy controls. The assay of SELDI-TOF-MS with analytical technique of protein-chip bioinformatics was used to detect the expression of protein mass peaks in the sera of patients or controls. One hundred serum samples, including 52 cases of colorectal cancer, 27 cases of colorectal benign disease, and 21 cases of healthy controls, were examined by SELDI-TOF-MS with WCX2 protein-chips. RESULTS: The diagnostic models (I, II and III) were setup by analyzed the data and sieved markers using Ciphergen - Protein-Chip-Software 5.1. These models were combined with 3 protein mass peaks to discriminate CRC, CBD, and healthy controls. The accuracy, the sensitivity and the particularity of cross verification of these models are all highly over 80%. CONCLUSIONS: The SELDI-TOF-MS is a useful tool to help diagnose colorectal cancer, especially during the early stage. However, identification of the significantly differentiated proteins needs further study. BioMed Central 2010-03-25 /pmc/articles/PMC2862023/ /pubmed/20334691 http://dx.doi.org/10.1186/1477-5956-8-16 Text en Copyright ©2010 Liu et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Liu, Ming Li, Chun-Feng Chen, Hong-Sheng Lin, Luo-Qiang Zhang, Chun-Peng Zhao, Jin-Lu Liu, Yan Zhang, Shu-Jun Jin, Jun-Chao Wang, Lei Liu, Jia-Ren Differential expression of proteomics models of colorectal cancer, colorectal benign disease and healthy controls |
title | Differential expression of proteomics models of colorectal cancer, colorectal benign disease and healthy controls |
title_full | Differential expression of proteomics models of colorectal cancer, colorectal benign disease and healthy controls |
title_fullStr | Differential expression of proteomics models of colorectal cancer, colorectal benign disease and healthy controls |
title_full_unstemmed | Differential expression of proteomics models of colorectal cancer, colorectal benign disease and healthy controls |
title_short | Differential expression of proteomics models of colorectal cancer, colorectal benign disease and healthy controls |
title_sort | differential expression of proteomics models of colorectal cancer, colorectal benign disease and healthy controls |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2862023/ https://www.ncbi.nlm.nih.gov/pubmed/20334691 http://dx.doi.org/10.1186/1477-5956-8-16 |
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