Using buoyant mass to measure the growth of single cells

We used a suspended microchannel resonator (SMR) combined with picoliter-scale microfluidic control to measure buoyant mass and determine the ‘instantaneous’ growth rates of individual cells. The SMR measures mass with femtogram precision, allowing rapid determination of the growth rate in a fractio...

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Detalles Bibliográficos
Autores principales: Godin, Michel, Delgado, Francisco Feijó, Son, Sungmin, Grover, William H., Bryan, Andrea K., Tzur, Amit, Jorgensen, Paul, Payer, Kris, Grossman, Alan D., Kirschner, Marc W., Manalis, Scott R.
Formato: Texto
Lenguaje:English
Publicado: 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2862099/
https://www.ncbi.nlm.nih.gov/pubmed/20383132
http://dx.doi.org/10.1038/nmeth.1452
Descripción
Sumario:We used a suspended microchannel resonator (SMR) combined with picoliter-scale microfluidic control to measure buoyant mass and determine the ‘instantaneous’ growth rates of individual cells. The SMR measures mass with femtogram precision, allowing rapid determination of the growth rate in a fraction of a complete cell cycle. We found that for individual cells of Bacillus subtilis, Escherichia coli, Saccharomyces cerevisiae and mouse lymphoblasts, heavier cells grew faster than lighter cells.

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