Using buoyant mass to measure the growth of single cells

We used a suspended microchannel resonator (SMR) combined with picoliter-scale microfluidic control to measure buoyant mass and determine the ‘instantaneous’ growth rates of individual cells. The SMR measures mass with femtogram precision, allowing rapid determination of the growth rate in a fractio...

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Detalles Bibliográficos
Autores principales: Godin, Michel, Delgado, Francisco Feijó, Son, Sungmin, Grover, William H., Bryan, Andrea K., Tzur, Amit, Jorgensen, Paul, Payer, Kris, Grossman, Alan D., Kirschner, Marc W., Manalis, Scott R.
Formato: Texto
Lenguaje:English
Publicado: 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2862099/
https://www.ncbi.nlm.nih.gov/pubmed/20383132
http://dx.doi.org/10.1038/nmeth.1452
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author Godin, Michel
Delgado, Francisco Feijó
Son, Sungmin
Grover, William H.
Bryan, Andrea K.
Tzur, Amit
Jorgensen, Paul
Payer, Kris
Grossman, Alan D.
Kirschner, Marc W.
Manalis, Scott R.
author_facet Godin, Michel
Delgado, Francisco Feijó
Son, Sungmin
Grover, William H.
Bryan, Andrea K.
Tzur, Amit
Jorgensen, Paul
Payer, Kris
Grossman, Alan D.
Kirschner, Marc W.
Manalis, Scott R.
author_sort Godin, Michel
collection PubMed
description We used a suspended microchannel resonator (SMR) combined with picoliter-scale microfluidic control to measure buoyant mass and determine the ‘instantaneous’ growth rates of individual cells. The SMR measures mass with femtogram precision, allowing rapid determination of the growth rate in a fraction of a complete cell cycle. We found that for individual cells of Bacillus subtilis, Escherichia coli, Saccharomyces cerevisiae and mouse lymphoblasts, heavier cells grew faster than lighter cells.
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spelling pubmed-28620992010-11-01 Using buoyant mass to measure the growth of single cells Godin, Michel Delgado, Francisco Feijó Son, Sungmin Grover, William H. Bryan, Andrea K. Tzur, Amit Jorgensen, Paul Payer, Kris Grossman, Alan D. Kirschner, Marc W. Manalis, Scott R. Nat Methods Article We used a suspended microchannel resonator (SMR) combined with picoliter-scale microfluidic control to measure buoyant mass and determine the ‘instantaneous’ growth rates of individual cells. The SMR measures mass with femtogram precision, allowing rapid determination of the growth rate in a fraction of a complete cell cycle. We found that for individual cells of Bacillus subtilis, Escherichia coli, Saccharomyces cerevisiae and mouse lymphoblasts, heavier cells grew faster than lighter cells. 2010-04-11 2010-05 /pmc/articles/PMC2862099/ /pubmed/20383132 http://dx.doi.org/10.1038/nmeth.1452 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Godin, Michel
Delgado, Francisco Feijó
Son, Sungmin
Grover, William H.
Bryan, Andrea K.
Tzur, Amit
Jorgensen, Paul
Payer, Kris
Grossman, Alan D.
Kirschner, Marc W.
Manalis, Scott R.
Using buoyant mass to measure the growth of single cells
title Using buoyant mass to measure the growth of single cells
title_full Using buoyant mass to measure the growth of single cells
title_fullStr Using buoyant mass to measure the growth of single cells
title_full_unstemmed Using buoyant mass to measure the growth of single cells
title_short Using buoyant mass to measure the growth of single cells
title_sort using buoyant mass to measure the growth of single cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2862099/
https://www.ncbi.nlm.nih.gov/pubmed/20383132
http://dx.doi.org/10.1038/nmeth.1452
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