Cargando…

Control of flowering time and spike development in cereals: the earliness per se Eps-1 region in wheat, rice, and Brachypodium

The earliness per se gene Eps-A (m) 1 from diploid wheat Triticum monococcum affects heading time, spike development, and spikelet number. In this study, the Eps1 orthologous regions from rice, Aegilops tauschii, and Brachypodium distachyon were compared as part of current efforts to clone this gene...

Descripción completa

Detalles Bibliográficos
Autores principales: Faricelli, Maria E., Valárik, Miroslav, Dubcovsky, Jorge
Formato: Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2862174/
https://www.ncbi.nlm.nih.gov/pubmed/19851796
http://dx.doi.org/10.1007/s10142-009-0146-7
_version_ 1782180698259456000
author Faricelli, Maria E.
Valárik, Miroslav
Dubcovsky, Jorge
author_facet Faricelli, Maria E.
Valárik, Miroslav
Dubcovsky, Jorge
author_sort Faricelli, Maria E.
collection PubMed
description The earliness per se gene Eps-A (m) 1 from diploid wheat Triticum monococcum affects heading time, spike development, and spikelet number. In this study, the Eps1 orthologous regions from rice, Aegilops tauschii, and Brachypodium distachyon were compared as part of current efforts to clone this gene. A single Brachypodium BAC clone spanned the Eps-A (m) 1 region, but a gap was detected in the A. tauschii physical map. Sequencing of the Brachypodium and A. tauschii BAC clones revealed three genes shared by the three species, which showed higher identity between wheat and Brachypodium than between them and rice. However, most of the structural changes were detected in the wheat lineage. These included an inversion encompassing the wg241-VatpC region and the presence of six unique genes. In contrast, only one unique gene (and one pseudogene) was found in Brachypodium and none in rice. Three genes were present in both Brachypodium and wheat but were absent in rice. Two of these genes, Mot1 and FtsH4, were completely linked to the earliness per se phenotype in the T. monococcum high-density genetic map and are candidates for Eps-A (m) 1. Both genes were expressed in apices and developing spikes, as expected for Eps-A (m) 1 candidates. The predicted MOT1 protein showed amino acid differences between the parental T. monococcum lines, but its effect is difficult to predict. Future steps to clone the Eps-A (m) 1 gene include the generation of mot1 and ftsh4 mutants and the completion of the T. monococcum physical map to test for the presence of additional candidate genes. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s10142-009-0146-7) contains supplementary material, which is available to authorized users.
format Text
id pubmed-2862174
institution National Center for Biotechnology Information
language English
publishDate 2009
publisher Springer Berlin Heidelberg
record_format MEDLINE/PubMed
spelling pubmed-28621742010-05-10 Control of flowering time and spike development in cereals: the earliness per se Eps-1 region in wheat, rice, and Brachypodium Faricelli, Maria E. Valárik, Miroslav Dubcovsky, Jorge Funct Integr Genomics Original Paper The earliness per se gene Eps-A (m) 1 from diploid wheat Triticum monococcum affects heading time, spike development, and spikelet number. In this study, the Eps1 orthologous regions from rice, Aegilops tauschii, and Brachypodium distachyon were compared as part of current efforts to clone this gene. A single Brachypodium BAC clone spanned the Eps-A (m) 1 region, but a gap was detected in the A. tauschii physical map. Sequencing of the Brachypodium and A. tauschii BAC clones revealed three genes shared by the three species, which showed higher identity between wheat and Brachypodium than between them and rice. However, most of the structural changes were detected in the wheat lineage. These included an inversion encompassing the wg241-VatpC region and the presence of six unique genes. In contrast, only one unique gene (and one pseudogene) was found in Brachypodium and none in rice. Three genes were present in both Brachypodium and wheat but were absent in rice. Two of these genes, Mot1 and FtsH4, were completely linked to the earliness per se phenotype in the T. monococcum high-density genetic map and are candidates for Eps-A (m) 1. Both genes were expressed in apices and developing spikes, as expected for Eps-A (m) 1 candidates. The predicted MOT1 protein showed amino acid differences between the parental T. monococcum lines, but its effect is difficult to predict. Future steps to clone the Eps-A (m) 1 gene include the generation of mot1 and ftsh4 mutants and the completion of the T. monococcum physical map to test for the presence of additional candidate genes. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s10142-009-0146-7) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2009-10-23 2010 /pmc/articles/PMC2862174/ /pubmed/19851796 http://dx.doi.org/10.1007/s10142-009-0146-7 Text en © The Author(s) 2009 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Original Paper
Faricelli, Maria E.
Valárik, Miroslav
Dubcovsky, Jorge
Control of flowering time and spike development in cereals: the earliness per se Eps-1 region in wheat, rice, and Brachypodium
title Control of flowering time and spike development in cereals: the earliness per se Eps-1 region in wheat, rice, and Brachypodium
title_full Control of flowering time and spike development in cereals: the earliness per se Eps-1 region in wheat, rice, and Brachypodium
title_fullStr Control of flowering time and spike development in cereals: the earliness per se Eps-1 region in wheat, rice, and Brachypodium
title_full_unstemmed Control of flowering time and spike development in cereals: the earliness per se Eps-1 region in wheat, rice, and Brachypodium
title_short Control of flowering time and spike development in cereals: the earliness per se Eps-1 region in wheat, rice, and Brachypodium
title_sort control of flowering time and spike development in cereals: the earliness per se eps-1 region in wheat, rice, and brachypodium
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2862174/
https://www.ncbi.nlm.nih.gov/pubmed/19851796
http://dx.doi.org/10.1007/s10142-009-0146-7
work_keys_str_mv AT faricellimariae controloffloweringtimeandspikedevelopmentincerealstheearlinessperseeps1regioninwheatriceandbrachypodium
AT valarikmiroslav controloffloweringtimeandspikedevelopmentincerealstheearlinessperseeps1regioninwheatriceandbrachypodium
AT dubcovskyjorge controloffloweringtimeandspikedevelopmentincerealstheearlinessperseeps1regioninwheatriceandbrachypodium