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The membrane attack complex of complement drives the progression of atherosclerosis in apolipoprotein E knockout mice
AIMS: To examine the roles of the membrane attack complex of complement and its sole membrane regulator, CD59, in atherosclerosis. METHODS: C6 (C6(−/−)) deficient and CD59a (Cd59a(−/−)) knockout mice were separately crossed onto the apolipoprotein E knockout (apoE(−/−)) background. The double knocko...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Pergamon Press
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2862291/ https://www.ncbi.nlm.nih.gov/pubmed/19959238 http://dx.doi.org/10.1016/j.molimm.2009.10.035 |
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author | Lewis, Ruth D. Jackson, Christopher L. Morgan, B. Paul Hughes, Timothy R. |
author_facet | Lewis, Ruth D. Jackson, Christopher L. Morgan, B. Paul Hughes, Timothy R. |
author_sort | Lewis, Ruth D. |
collection | PubMed |
description | AIMS: To examine the roles of the membrane attack complex of complement and its sole membrane regulator, CD59, in atherosclerosis. METHODS: C6 (C6(−/−)) deficient and CD59a (Cd59a(−/−)) knockout mice were separately crossed onto the apolipoprotein E knockout (apoE(−/−)) background. The double knockout mice were fed high-fat diet in order to study the effects of absence of C6 or CD59a on the progression of atherosclerosis. RESULTS: C6 deficiency significantly reduced plaque area and disease severity. CD59a had the opposite effect in that deficiency was associated with a significant increase in plaque area, correlating with increased membrane attack complex (MAC) deposition in the plaque and increased smooth muscle cell proliferation in early plaques. CONCLUSIONS: Our results demonstrate that the MAC contributes to the development of atherosclerosis, C6 deficiency being protective and CD59a deficiency exacerbating disease. |
format | Text |
id | pubmed-2862291 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Pergamon Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-28622912010-05-25 The membrane attack complex of complement drives the progression of atherosclerosis in apolipoprotein E knockout mice Lewis, Ruth D. Jackson, Christopher L. Morgan, B. Paul Hughes, Timothy R. Mol Immunol Article AIMS: To examine the roles of the membrane attack complex of complement and its sole membrane regulator, CD59, in atherosclerosis. METHODS: C6 (C6(−/−)) deficient and CD59a (Cd59a(−/−)) knockout mice were separately crossed onto the apolipoprotein E knockout (apoE(−/−)) background. The double knockout mice were fed high-fat diet in order to study the effects of absence of C6 or CD59a on the progression of atherosclerosis. RESULTS: C6 deficiency significantly reduced plaque area and disease severity. CD59a had the opposite effect in that deficiency was associated with a significant increase in plaque area, correlating with increased membrane attack complex (MAC) deposition in the plaque and increased smooth muscle cell proliferation in early plaques. CONCLUSIONS: Our results demonstrate that the MAC contributes to the development of atherosclerosis, C6 deficiency being protective and CD59a deficiency exacerbating disease. Pergamon Press 2010-02 /pmc/articles/PMC2862291/ /pubmed/19959238 http://dx.doi.org/10.1016/j.molimm.2009.10.035 Text en © 2010 Elsevier Ltd. https://creativecommons.org/licenses/by-nc-nd/3.0/ Open Access under CC BY-NC-ND 3.0 (https://creativecommons.org/licenses/by-nc-nd/3.0/) license |
spellingShingle | Article Lewis, Ruth D. Jackson, Christopher L. Morgan, B. Paul Hughes, Timothy R. The membrane attack complex of complement drives the progression of atherosclerosis in apolipoprotein E knockout mice |
title | The membrane attack complex of complement drives the progression of atherosclerosis in apolipoprotein E knockout mice |
title_full | The membrane attack complex of complement drives the progression of atherosclerosis in apolipoprotein E knockout mice |
title_fullStr | The membrane attack complex of complement drives the progression of atherosclerosis in apolipoprotein E knockout mice |
title_full_unstemmed | The membrane attack complex of complement drives the progression of atherosclerosis in apolipoprotein E knockout mice |
title_short | The membrane attack complex of complement drives the progression of atherosclerosis in apolipoprotein E knockout mice |
title_sort | membrane attack complex of complement drives the progression of atherosclerosis in apolipoprotein e knockout mice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2862291/ https://www.ncbi.nlm.nih.gov/pubmed/19959238 http://dx.doi.org/10.1016/j.molimm.2009.10.035 |
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