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Receptor-Mediated Transcytosis of Leptin through Human Intestinal Cells In Vitro

Gastric Leptin is absorbed by duodenal enterocytes and released on the basolateral side towards the bloodstream. We investigated in vitro some of the mechanisms of this transport. Caco-2/15 cells internalize leptin from the apical medium and release it through transcytosis in the basal medium in a t...

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Autores principales: Cammisotto, Philippe G., Bendayan, Moise, Sané, Alain, Dominguez, Michel, Garofalo, Carole, Levy, Émile
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2862316/
https://www.ncbi.nlm.nih.gov/pubmed/20454702
http://dx.doi.org/10.1155/2010/928169
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author Cammisotto, Philippe G.
Bendayan, Moise
Sané, Alain
Dominguez, Michel
Garofalo, Carole
Levy, Émile
author_facet Cammisotto, Philippe G.
Bendayan, Moise
Sané, Alain
Dominguez, Michel
Garofalo, Carole
Levy, Émile
author_sort Cammisotto, Philippe G.
collection PubMed
description Gastric Leptin is absorbed by duodenal enterocytes and released on the basolateral side towards the bloodstream. We investigated in vitro some of the mechanisms of this transport. Caco-2/15 cells internalize leptin from the apical medium and release it through transcytosis in the basal medium in a time- temperature-dependent and saturable fashion. Leptin receptors are revealed on the apical brush-border membrane of the Caco-2 cells. RNA-mediated silencing of the receptor led to decreases in the uptake and basolateral release. Leptin in the basal medium was found bound to the soluble form of its receptor. An inhibitor of clathrin-dependent endocytosis (chlorpromazine) decreased leptin uptake. Confocal immunocytochemistry and the use of brefeldin A and okadaic acid revealed the passage of leptin through the Golgi apparatus. We propose that leptin transcytosis by intestinal cells depends on its receptor, on clathrin-coated vesicles and transits through the Golgi apparatus.
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spelling pubmed-28623162010-05-07 Receptor-Mediated Transcytosis of Leptin through Human Intestinal Cells In Vitro Cammisotto, Philippe G. Bendayan, Moise Sané, Alain Dominguez, Michel Garofalo, Carole Levy, Émile Int J Cell Biol Research Article Gastric Leptin is absorbed by duodenal enterocytes and released on the basolateral side towards the bloodstream. We investigated in vitro some of the mechanisms of this transport. Caco-2/15 cells internalize leptin from the apical medium and release it through transcytosis in the basal medium in a time- temperature-dependent and saturable fashion. Leptin receptors are revealed on the apical brush-border membrane of the Caco-2 cells. RNA-mediated silencing of the receptor led to decreases in the uptake and basolateral release. Leptin in the basal medium was found bound to the soluble form of its receptor. An inhibitor of clathrin-dependent endocytosis (chlorpromazine) decreased leptin uptake. Confocal immunocytochemistry and the use of brefeldin A and okadaic acid revealed the passage of leptin through the Golgi apparatus. We propose that leptin transcytosis by intestinal cells depends on its receptor, on clathrin-coated vesicles and transits through the Golgi apparatus. Hindawi Publishing Corporation 2010 2010-04-29 /pmc/articles/PMC2862316/ /pubmed/20454702 http://dx.doi.org/10.1155/2010/928169 Text en Copyright © 2010 Philippe G. Cammisotto et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Cammisotto, Philippe G.
Bendayan, Moise
Sané, Alain
Dominguez, Michel
Garofalo, Carole
Levy, Émile
Receptor-Mediated Transcytosis of Leptin through Human Intestinal Cells In Vitro
title Receptor-Mediated Transcytosis of Leptin through Human Intestinal Cells In Vitro
title_full Receptor-Mediated Transcytosis of Leptin through Human Intestinal Cells In Vitro
title_fullStr Receptor-Mediated Transcytosis of Leptin through Human Intestinal Cells In Vitro
title_full_unstemmed Receptor-Mediated Transcytosis of Leptin through Human Intestinal Cells In Vitro
title_short Receptor-Mediated Transcytosis of Leptin through Human Intestinal Cells In Vitro
title_sort receptor-mediated transcytosis of leptin through human intestinal cells in vitro
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2862316/
https://www.ncbi.nlm.nih.gov/pubmed/20454702
http://dx.doi.org/10.1155/2010/928169
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