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Effect of low tidal volume ventilation on lung function and inflammation in mice

BACKGROUND: A large number of studies have investigated the effects of high tidal volume ventilation in mouse models. In contrast data on very short term effects of low tidal volume ventilation are sparse. Therefore we investigated the functional and structural effects of low tidal volume ventilatio...

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Autores principales: Hauber, Hans P, Karp, Dörte, Goldmann, Torsten, Vollmer, Ekkehard, Zabel, Peter
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2864238/
https://www.ncbi.nlm.nih.gov/pubmed/20409304
http://dx.doi.org/10.1186/1471-2466-10-21
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author Hauber, Hans P
Karp, Dörte
Goldmann, Torsten
Vollmer, Ekkehard
Zabel, Peter
author_facet Hauber, Hans P
Karp, Dörte
Goldmann, Torsten
Vollmer, Ekkehard
Zabel, Peter
author_sort Hauber, Hans P
collection PubMed
description BACKGROUND: A large number of studies have investigated the effects of high tidal volume ventilation in mouse models. In contrast data on very short term effects of low tidal volume ventilation are sparse. Therefore we investigated the functional and structural effects of low tidal volume ventilation in mice. METHODS: 38 Male C57/Bl6 mice were ventilated with different tidal volumes (Vt 5, 7, and 10 ml/kg) without or with application of PEEP (2 cm H(2)O). Four spontaneously breathing animals served as controls. Oxygen saturation and pulse rate were monitored. Lung function was measured every 5 min for at least 30 min. Afterwards lungs were removed and histological sections were stained for measurement of infiltration with polymorphonuclear leukocytes (PMN). Moreover, mRNA expression of macrophage inflammatory protein (MIP)-2 and tumor necrosis factor (TNF)α in the lungs was quantified using real time PCR. RESULTS: Oxygen saturation did not change significantly over time of ventilation in all groups (P > 0.05). Pulse rate dropped in all groups without PEEP during mechanical ventilation. In contrast, in the groups with PEEP pulse rate increased over time. These effects were not statistically significant (P > 0.05). Tissue damping (G) and tissue elastance (H) were significantly increased in all groups after 30 min of ventilation (P < 0.05). Only the group with a Vt of 10 ml/kg and PEEP did not show a significant increase in H (P > 0.05). Mechanical ventilation significantly increased infiltration of the lungs with PMN (P < 0.05). Expression of MIP-2 was significantly induced by mechanical ventilation in all groups (P < 0.05). MIP-2 mRNA expression was lowest in the group with a Vt of 10 ml/kg + PEEP. CONCLUSIONS: Our data show that very short term mechanical ventilation with lower tidal volumes than 10 ml/kg did not reduce inflammation additionally. Formation of atelectasis and inadequate oxygenation with very low tidal volumes may be important factors. Application of PEEP attenuated inflammation.
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spelling pubmed-28642382010-05-05 Effect of low tidal volume ventilation on lung function and inflammation in mice Hauber, Hans P Karp, Dörte Goldmann, Torsten Vollmer, Ekkehard Zabel, Peter BMC Pulm Med Research article BACKGROUND: A large number of studies have investigated the effects of high tidal volume ventilation in mouse models. In contrast data on very short term effects of low tidal volume ventilation are sparse. Therefore we investigated the functional and structural effects of low tidal volume ventilation in mice. METHODS: 38 Male C57/Bl6 mice were ventilated with different tidal volumes (Vt 5, 7, and 10 ml/kg) without or with application of PEEP (2 cm H(2)O). Four spontaneously breathing animals served as controls. Oxygen saturation and pulse rate were monitored. Lung function was measured every 5 min for at least 30 min. Afterwards lungs were removed and histological sections were stained for measurement of infiltration with polymorphonuclear leukocytes (PMN). Moreover, mRNA expression of macrophage inflammatory protein (MIP)-2 and tumor necrosis factor (TNF)α in the lungs was quantified using real time PCR. RESULTS: Oxygen saturation did not change significantly over time of ventilation in all groups (P > 0.05). Pulse rate dropped in all groups without PEEP during mechanical ventilation. In contrast, in the groups with PEEP pulse rate increased over time. These effects were not statistically significant (P > 0.05). Tissue damping (G) and tissue elastance (H) were significantly increased in all groups after 30 min of ventilation (P < 0.05). Only the group with a Vt of 10 ml/kg and PEEP did not show a significant increase in H (P > 0.05). Mechanical ventilation significantly increased infiltration of the lungs with PMN (P < 0.05). Expression of MIP-2 was significantly induced by mechanical ventilation in all groups (P < 0.05). MIP-2 mRNA expression was lowest in the group with a Vt of 10 ml/kg + PEEP. CONCLUSIONS: Our data show that very short term mechanical ventilation with lower tidal volumes than 10 ml/kg did not reduce inflammation additionally. Formation of atelectasis and inadequate oxygenation with very low tidal volumes may be important factors. Application of PEEP attenuated inflammation. BioMed Central 2010-04-21 /pmc/articles/PMC2864238/ /pubmed/20409304 http://dx.doi.org/10.1186/1471-2466-10-21 Text en Copyright ©2010 Hauber et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research article
Hauber, Hans P
Karp, Dörte
Goldmann, Torsten
Vollmer, Ekkehard
Zabel, Peter
Effect of low tidal volume ventilation on lung function and inflammation in mice
title Effect of low tidal volume ventilation on lung function and inflammation in mice
title_full Effect of low tidal volume ventilation on lung function and inflammation in mice
title_fullStr Effect of low tidal volume ventilation on lung function and inflammation in mice
title_full_unstemmed Effect of low tidal volume ventilation on lung function and inflammation in mice
title_short Effect of low tidal volume ventilation on lung function and inflammation in mice
title_sort effect of low tidal volume ventilation on lung function and inflammation in mice
topic Research article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2864238/
https://www.ncbi.nlm.nih.gov/pubmed/20409304
http://dx.doi.org/10.1186/1471-2466-10-21
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