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Evolutionary conservation of essential and highly expressed genes in Pseudomonas aeruginosa

BACKGROUND: The constant increase in development and spread of bacterial resistance to antibiotics poses a serious threat to human health. New sequencing technologies are now on the horizon that will yield massive increases in our capacity for DNA sequencing and will revolutionize the drug discovery...

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Autores principales: Dötsch, Andreas, Klawonn, Frank, Jarek, Michael, Scharfe, Maren, Blöcker, Helmut, Häussler, Susanne
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2864246/
https://www.ncbi.nlm.nih.gov/pubmed/20380691
http://dx.doi.org/10.1186/1471-2164-11-234
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author Dötsch, Andreas
Klawonn, Frank
Jarek, Michael
Scharfe, Maren
Blöcker, Helmut
Häussler, Susanne
author_facet Dötsch, Andreas
Klawonn, Frank
Jarek, Michael
Scharfe, Maren
Blöcker, Helmut
Häussler, Susanne
author_sort Dötsch, Andreas
collection PubMed
description BACKGROUND: The constant increase in development and spread of bacterial resistance to antibiotics poses a serious threat to human health. New sequencing technologies are now on the horizon that will yield massive increases in our capacity for DNA sequencing and will revolutionize the drug discovery process. Since essential genes are promising novel antibiotic targets, the prediction of gene essentiality based on genomic information has become a major focus. RESULTS: In this study we demonstrate that pooled sequencing is applicable for the analysis of sequence variations of strain collections with more than 10 individual isolates. Pooled sequencing of 36 clinical Pseudomonas aeruginosa isolates revealed that essential and highly expressed proteins evolve at lower rates, whereas extracellular proteins evolve at higher rates. We furthermore refined the list of experimentally essential P. aeruginosa genes, and identified 980 genes that show no sequence variation at all. Among the conserved nonessential genes we found several that are involved in regulation, motility and virulence, indicating that they represent factors of evolutionary importance for the lifestyle of a successful environmental bacterium and opportunistic pathogen. CONCLUSION: The detailed analysis of a comprehensive set of P. aeruginosa genomes in this study clearly disclosed detailed information of the genomic makeup and revealed a large set of highly conserved genes that play an important role for the lifestyle of this microorganism. Sequencing strain collections enables for a detailed and extensive identification of sequence variations as potential bacterial adaptation processes, e.g., during the development of antibiotic resistance in the clinical setting and thus may be the basis to uncover putative targets for novel treatment strategies.
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spelling pubmed-28642462010-05-05 Evolutionary conservation of essential and highly expressed genes in Pseudomonas aeruginosa Dötsch, Andreas Klawonn, Frank Jarek, Michael Scharfe, Maren Blöcker, Helmut Häussler, Susanne BMC Genomics Research Article BACKGROUND: The constant increase in development and spread of bacterial resistance to antibiotics poses a serious threat to human health. New sequencing technologies are now on the horizon that will yield massive increases in our capacity for DNA sequencing and will revolutionize the drug discovery process. Since essential genes are promising novel antibiotic targets, the prediction of gene essentiality based on genomic information has become a major focus. RESULTS: In this study we demonstrate that pooled sequencing is applicable for the analysis of sequence variations of strain collections with more than 10 individual isolates. Pooled sequencing of 36 clinical Pseudomonas aeruginosa isolates revealed that essential and highly expressed proteins evolve at lower rates, whereas extracellular proteins evolve at higher rates. We furthermore refined the list of experimentally essential P. aeruginosa genes, and identified 980 genes that show no sequence variation at all. Among the conserved nonessential genes we found several that are involved in regulation, motility and virulence, indicating that they represent factors of evolutionary importance for the lifestyle of a successful environmental bacterium and opportunistic pathogen. CONCLUSION: The detailed analysis of a comprehensive set of P. aeruginosa genomes in this study clearly disclosed detailed information of the genomic makeup and revealed a large set of highly conserved genes that play an important role for the lifestyle of this microorganism. Sequencing strain collections enables for a detailed and extensive identification of sequence variations as potential bacterial adaptation processes, e.g., during the development of antibiotic resistance in the clinical setting and thus may be the basis to uncover putative targets for novel treatment strategies. BioMed Central 2010-04-09 /pmc/articles/PMC2864246/ /pubmed/20380691 http://dx.doi.org/10.1186/1471-2164-11-234 Text en Copyright ©2010 Dötsch et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Dötsch, Andreas
Klawonn, Frank
Jarek, Michael
Scharfe, Maren
Blöcker, Helmut
Häussler, Susanne
Evolutionary conservation of essential and highly expressed genes in Pseudomonas aeruginosa
title Evolutionary conservation of essential and highly expressed genes in Pseudomonas aeruginosa
title_full Evolutionary conservation of essential and highly expressed genes in Pseudomonas aeruginosa
title_fullStr Evolutionary conservation of essential and highly expressed genes in Pseudomonas aeruginosa
title_full_unstemmed Evolutionary conservation of essential and highly expressed genes in Pseudomonas aeruginosa
title_short Evolutionary conservation of essential and highly expressed genes in Pseudomonas aeruginosa
title_sort evolutionary conservation of essential and highly expressed genes in pseudomonas aeruginosa
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2864246/
https://www.ncbi.nlm.nih.gov/pubmed/20380691
http://dx.doi.org/10.1186/1471-2164-11-234
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