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Production of enterodiol from defatted flaxseeds through biotransformation by human intestinal bacteria
BACKGROUND: The effects of enterolignans, e.g., enterodiol (END) and particularly its oxidation product, enterolactone (ENL), on prevention of hormone-dependent diseases, such as osteoporosis, cardiovascular diseases, hyperlipemia, breast cancer, colon cancer, prostate cancer and menopausal syndrome...
Autores principales: | , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2865466/ https://www.ncbi.nlm.nih.gov/pubmed/20398397 http://dx.doi.org/10.1186/1471-2180-10-115 |
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author | Wang, Cheng-Zhi Ma, Xiao-Qing Yang, Dong-Hui Guo, Zhi-Rong Liu, Gui-Rong Zhao, Ge-Xin Tang, Jie Zhang, Ya-Nan Ma, Miao Cai, Shao-Qing Ku, Bao-Shan Liu, Shu-Lin |
author_facet | Wang, Cheng-Zhi Ma, Xiao-Qing Yang, Dong-Hui Guo, Zhi-Rong Liu, Gui-Rong Zhao, Ge-Xin Tang, Jie Zhang, Ya-Nan Ma, Miao Cai, Shao-Qing Ku, Bao-Shan Liu, Shu-Lin |
author_sort | Wang, Cheng-Zhi |
collection | PubMed |
description | BACKGROUND: The effects of enterolignans, e.g., enterodiol (END) and particularly its oxidation product, enterolactone (ENL), on prevention of hormone-dependent diseases, such as osteoporosis, cardiovascular diseases, hyperlipemia, breast cancer, colon cancer, prostate cancer and menopausal syndrome, have attracted much attention. To date, the main way to obtain END and ENL is chemical synthesis, which is expensive and inevitably leads to environmental pollution. To explore a more economic and eco-friendly production method, we explored biotransformation of enterolignans from precursors contained in defatted flaxseeds by human intestinal bacteria. RESULTS: We cultured fecal specimens from healthy young adults in media containing defatted flaxseeds and detected END from the culture supernatant. Following selection through successive subcultures of the fecal microbiota with defatted flaxseeds as the only carbon source, we obtained a bacterial consortium, designated as END-49, which contained the smallest number of bacterial types still capable of metabolizing defatted flaxseeds to produce END. Based on analysis with pulsed field gel electrophoresis, END-49 was found to consist of five genomically distinct bacterial lineages, designated Group I-V, with Group I strains dominating the culture. None of the individual Group I-V strains produced END, demonstrating that the biotransformation of substrates in defatted flaxseeds into END is a joint work by different members of the END-49 bacterial consortium. Interestingly, Group I strains produced secoisolariciresinol, an important intermediate of END production; 16S rRNA analysis of one Group I strain established its close relatedness with Klebsiella. Genomic analysis is under way to identify all members in END-49 involved in the biotransformation and the actual pathway leading to END-production. CONCLUSION: Biotransformation is a very economic, efficient and environmentally friendly way of mass-producing enterodiol from defatted flaxseeds. |
format | Text |
id | pubmed-2865466 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-28654662010-05-07 Production of enterodiol from defatted flaxseeds through biotransformation by human intestinal bacteria Wang, Cheng-Zhi Ma, Xiao-Qing Yang, Dong-Hui Guo, Zhi-Rong Liu, Gui-Rong Zhao, Ge-Xin Tang, Jie Zhang, Ya-Nan Ma, Miao Cai, Shao-Qing Ku, Bao-Shan Liu, Shu-Lin BMC Microbiol Research article BACKGROUND: The effects of enterolignans, e.g., enterodiol (END) and particularly its oxidation product, enterolactone (ENL), on prevention of hormone-dependent diseases, such as osteoporosis, cardiovascular diseases, hyperlipemia, breast cancer, colon cancer, prostate cancer and menopausal syndrome, have attracted much attention. To date, the main way to obtain END and ENL is chemical synthesis, which is expensive and inevitably leads to environmental pollution. To explore a more economic and eco-friendly production method, we explored biotransformation of enterolignans from precursors contained in defatted flaxseeds by human intestinal bacteria. RESULTS: We cultured fecal specimens from healthy young adults in media containing defatted flaxseeds and detected END from the culture supernatant. Following selection through successive subcultures of the fecal microbiota with defatted flaxseeds as the only carbon source, we obtained a bacterial consortium, designated as END-49, which contained the smallest number of bacterial types still capable of metabolizing defatted flaxseeds to produce END. Based on analysis with pulsed field gel electrophoresis, END-49 was found to consist of five genomically distinct bacterial lineages, designated Group I-V, with Group I strains dominating the culture. None of the individual Group I-V strains produced END, demonstrating that the biotransformation of substrates in defatted flaxseeds into END is a joint work by different members of the END-49 bacterial consortium. Interestingly, Group I strains produced secoisolariciresinol, an important intermediate of END production; 16S rRNA analysis of one Group I strain established its close relatedness with Klebsiella. Genomic analysis is under way to identify all members in END-49 involved in the biotransformation and the actual pathway leading to END-production. CONCLUSION: Biotransformation is a very economic, efficient and environmentally friendly way of mass-producing enterodiol from defatted flaxseeds. BioMed Central 2010-04-16 /pmc/articles/PMC2865466/ /pubmed/20398397 http://dx.doi.org/10.1186/1471-2180-10-115 Text en Copyright ©2010 Wang et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research article Wang, Cheng-Zhi Ma, Xiao-Qing Yang, Dong-Hui Guo, Zhi-Rong Liu, Gui-Rong Zhao, Ge-Xin Tang, Jie Zhang, Ya-Nan Ma, Miao Cai, Shao-Qing Ku, Bao-Shan Liu, Shu-Lin Production of enterodiol from defatted flaxseeds through biotransformation by human intestinal bacteria |
title | Production of enterodiol from defatted flaxseeds through biotransformation by human intestinal bacteria |
title_full | Production of enterodiol from defatted flaxseeds through biotransformation by human intestinal bacteria |
title_fullStr | Production of enterodiol from defatted flaxseeds through biotransformation by human intestinal bacteria |
title_full_unstemmed | Production of enterodiol from defatted flaxseeds through biotransformation by human intestinal bacteria |
title_short | Production of enterodiol from defatted flaxseeds through biotransformation by human intestinal bacteria |
title_sort | production of enterodiol from defatted flaxseeds through biotransformation by human intestinal bacteria |
topic | Research article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2865466/ https://www.ncbi.nlm.nih.gov/pubmed/20398397 http://dx.doi.org/10.1186/1471-2180-10-115 |
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