Cargando…

Quantitative expression analysis and prognostic significance of L-DOPA decarboxylase in colorectal adenocarcinoma

BACKGROUND: L-DOPA decarboxylase (DDC) is an enzyme that catalyses, mainly, the decarboxylation of L-DOPA to dopamine and was found to be involved in many malignancies. The aim of this study was to investigate the mRNA expression levels of the DDC gene and to evaluate its clinical utility in tissues...

Descripción completa

Detalles Bibliográficos
Autores principales: Kontos, C K, Papadopoulos, I N, Fragoulis, E G, Scorilas, A
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2865762/
https://www.ncbi.nlm.nih.gov/pubmed/20424616
http://dx.doi.org/10.1038/sj.bjc.6605654
_version_ 1782180866296905728
author Kontos, C K
Papadopoulos, I N
Fragoulis, E G
Scorilas, A
author_facet Kontos, C K
Papadopoulos, I N
Fragoulis, E G
Scorilas, A
author_sort Kontos, C K
collection PubMed
description BACKGROUND: L-DOPA decarboxylase (DDC) is an enzyme that catalyses, mainly, the decarboxylation of L-DOPA to dopamine and was found to be involved in many malignancies. The aim of this study was to investigate the mRNA expression levels of the DDC gene and to evaluate its clinical utility in tissues with colorectal adenocarcinoma. METHODS: Total RNA was isolated from colorectal adenocarcinoma tissues of 95 patients. After having tested RNA quality, we prepared cDNA by reverse transcription. Highly sensitive quantitative real-time PCR method for DDC mRNA quantification was developed using the SYBR Green chemistry. GAPDH served as a housekeeping gene. Relative quantification analysis was performed using the comparative C(T) method (2(−ΔΔC(T))). RESULTS: DDC mRNA expression varied remarkably among colorectal tumours examined in this study. High DDC mRNA expression levels were found in well-differentiated and Dukes’ stage A and B tumours. Kaplan–Meier survival curves showed that patients with DDC-positive tumours have significantly longer disease-free survival (P=0.009) and overall survival (P=0.027). In Cox regression analysis of the entire cohort of patients, negative DDC proved to be a significant predictor of reduced disease-free (P=0.021) and overall survival (P=0.047). CONCLUSIONS: The results of the study suggest that DDC mRNA expression may be regarded as a novel potential tissue biomarker in colorectal adenocarcinoma.
format Text
id pubmed-2865762
institution National Center for Biotechnology Information
language English
publishDate 2010
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-28657622011-04-27 Quantitative expression analysis and prognostic significance of L-DOPA decarboxylase in colorectal adenocarcinoma Kontos, C K Papadopoulos, I N Fragoulis, E G Scorilas, A Br J Cancer Molecular Diagnostics BACKGROUND: L-DOPA decarboxylase (DDC) is an enzyme that catalyses, mainly, the decarboxylation of L-DOPA to dopamine and was found to be involved in many malignancies. The aim of this study was to investigate the mRNA expression levels of the DDC gene and to evaluate its clinical utility in tissues with colorectal adenocarcinoma. METHODS: Total RNA was isolated from colorectal adenocarcinoma tissues of 95 patients. After having tested RNA quality, we prepared cDNA by reverse transcription. Highly sensitive quantitative real-time PCR method for DDC mRNA quantification was developed using the SYBR Green chemistry. GAPDH served as a housekeeping gene. Relative quantification analysis was performed using the comparative C(T) method (2(−ΔΔC(T))). RESULTS: DDC mRNA expression varied remarkably among colorectal tumours examined in this study. High DDC mRNA expression levels were found in well-differentiated and Dukes’ stage A and B tumours. Kaplan–Meier survival curves showed that patients with DDC-positive tumours have significantly longer disease-free survival (P=0.009) and overall survival (P=0.027). In Cox regression analysis of the entire cohort of patients, negative DDC proved to be a significant predictor of reduced disease-free (P=0.021) and overall survival (P=0.047). CONCLUSIONS: The results of the study suggest that DDC mRNA expression may be regarded as a novel potential tissue biomarker in colorectal adenocarcinoma. Nature Publishing Group 2010-04-27 2010-04-27 /pmc/articles/PMC2865762/ /pubmed/20424616 http://dx.doi.org/10.1038/sj.bjc.6605654 Text en Copyright © 2010 Cancer Research UK https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Molecular Diagnostics
Kontos, C K
Papadopoulos, I N
Fragoulis, E G
Scorilas, A
Quantitative expression analysis and prognostic significance of L-DOPA decarboxylase in colorectal adenocarcinoma
title Quantitative expression analysis and prognostic significance of L-DOPA decarboxylase in colorectal adenocarcinoma
title_full Quantitative expression analysis and prognostic significance of L-DOPA decarboxylase in colorectal adenocarcinoma
title_fullStr Quantitative expression analysis and prognostic significance of L-DOPA decarboxylase in colorectal adenocarcinoma
title_full_unstemmed Quantitative expression analysis and prognostic significance of L-DOPA decarboxylase in colorectal adenocarcinoma
title_short Quantitative expression analysis and prognostic significance of L-DOPA decarboxylase in colorectal adenocarcinoma
title_sort quantitative expression analysis and prognostic significance of l-dopa decarboxylase in colorectal adenocarcinoma
topic Molecular Diagnostics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2865762/
https://www.ncbi.nlm.nih.gov/pubmed/20424616
http://dx.doi.org/10.1038/sj.bjc.6605654
work_keys_str_mv AT kontosck quantitativeexpressionanalysisandprognosticsignificanceofldopadecarboxylaseincolorectaladenocarcinoma
AT papadopoulosin quantitativeexpressionanalysisandprognosticsignificanceofldopadecarboxylaseincolorectaladenocarcinoma
AT fragouliseg quantitativeexpressionanalysisandprognosticsignificanceofldopadecarboxylaseincolorectaladenocarcinoma
AT scorilasa quantitativeexpressionanalysisandprognosticsignificanceofldopadecarboxylaseincolorectaladenocarcinoma