Cargando…
Transcriptional Response Of E. coli Upon FimH-mediated Fimbrial Adhesion
Functionalities which may be genetically programmed into a bacterium are limited by its range of possible activities and its sensory capabilities. Therefore, enhancing the bacterial sensory repertoire is a crucial step for expanded utility in potential biomedical, industrial or environmental applica...
Autores principales: | , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Libertas Academica
2010
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2865769/ https://www.ncbi.nlm.nih.gov/pubmed/20458372 |
_version_ | 1782180868068999168 |
---|---|
author | Bhomkar, Prasanna Materi, Wayne Semenchenko, Valentyna Wishart, David S. |
author_facet | Bhomkar, Prasanna Materi, Wayne Semenchenko, Valentyna Wishart, David S. |
author_sort | Bhomkar, Prasanna |
collection | PubMed |
description | Functionalities which may be genetically programmed into a bacterium are limited by its range of possible activities and its sensory capabilities. Therefore, enhancing the bacterial sensory repertoire is a crucial step for expanded utility in potential biomedical, industrial or environmental applications. Using microarray and qRT-PCR analyses, we have investigated transcription in E. coli (strain CSH50) following FimH-mediated adhesion to biocompatible substrates. Specifically, wild-type FimH-mediated adhesion of E. coli to mannose agarose beads and His-tagged FimH-mediated adhesion to Ni(2+)-NTA beads both led to induction of ahpCF, dps, grxA and marRAB genes among bound cells relative to unbound cells. The strongly-induced genes are known to be regulated by OxyR or SoxS cytoplasmic redox sensors. Some differentially altered genes also overlapped with those implicated in biofilm formation. This study provides an insight into transcriptional events following FimH-mediated adhesion and may provide a platform for elucidation of the signaling circuit necessary for engineering a synthetic attachment response in E. coli. |
format | Text |
id | pubmed-2865769 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Libertas Academica |
record_format | MEDLINE/PubMed |
spelling | pubmed-28657692010-05-10 Transcriptional Response Of E. coli Upon FimH-mediated Fimbrial Adhesion Bhomkar, Prasanna Materi, Wayne Semenchenko, Valentyna Wishart, David S. Gene Regul Syst Bio Original Research Functionalities which may be genetically programmed into a bacterium are limited by its range of possible activities and its sensory capabilities. Therefore, enhancing the bacterial sensory repertoire is a crucial step for expanded utility in potential biomedical, industrial or environmental applications. Using microarray and qRT-PCR analyses, we have investigated transcription in E. coli (strain CSH50) following FimH-mediated adhesion to biocompatible substrates. Specifically, wild-type FimH-mediated adhesion of E. coli to mannose agarose beads and His-tagged FimH-mediated adhesion to Ni(2+)-NTA beads both led to induction of ahpCF, dps, grxA and marRAB genes among bound cells relative to unbound cells. The strongly-induced genes are known to be regulated by OxyR or SoxS cytoplasmic redox sensors. Some differentially altered genes also overlapped with those implicated in biofilm formation. This study provides an insight into transcriptional events following FimH-mediated adhesion and may provide a platform for elucidation of the signaling circuit necessary for engineering a synthetic attachment response in E. coli. Libertas Academica 2010-03-24 /pmc/articles/PMC2865769/ /pubmed/20458372 Text en © the authors(s), publisher and licensee Libertas Academica Ltd. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Original Research Bhomkar, Prasanna Materi, Wayne Semenchenko, Valentyna Wishart, David S. Transcriptional Response Of E. coli Upon FimH-mediated Fimbrial Adhesion |
title | Transcriptional Response Of E. coli Upon FimH-mediated Fimbrial Adhesion |
title_full | Transcriptional Response Of E. coli Upon FimH-mediated Fimbrial Adhesion |
title_fullStr | Transcriptional Response Of E. coli Upon FimH-mediated Fimbrial Adhesion |
title_full_unstemmed | Transcriptional Response Of E. coli Upon FimH-mediated Fimbrial Adhesion |
title_short | Transcriptional Response Of E. coli Upon FimH-mediated Fimbrial Adhesion |
title_sort | transcriptional response of e. coli upon fimh-mediated fimbrial adhesion |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2865769/ https://www.ncbi.nlm.nih.gov/pubmed/20458372 |
work_keys_str_mv | AT bhomkarprasanna transcriptionalresponseofecoliuponfimhmediatedfimbrialadhesion AT materiwayne transcriptionalresponseofecoliuponfimhmediatedfimbrialadhesion AT semenchenkovalentyna transcriptionalresponseofecoliuponfimhmediatedfimbrialadhesion AT wishartdavids transcriptionalresponseofecoliuponfimhmediatedfimbrialadhesion |