HPLC Method for Estimation of Metformin Hydrochloride in Formulated Microspheres and Tablet Dosage Form

A simple, accurate, economical and reproducible HPLC method has been developed for quantitative estimation of metformin hydrochloride from tablet dosage form and formulated microspheres. The developed HPLC method is a reverse phase chromatographic method using phenomenex C(18) column and acetonitril...

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Detalles Bibliográficos
Autores principales: Kar, Mousumi, Choudhury, P. K.
Formato: Texto
Lenguaje:English
Publicado: Medknow Publications 2009
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2865795/
https://www.ncbi.nlm.nih.gov/pubmed/20490303
http://dx.doi.org/10.4103/0250-474X.56031
Descripción
Sumario:A simple, accurate, economical and reproducible HPLC method has been developed for quantitative estimation of metformin hydrochloride from tablet dosage form and formulated microspheres. The developed HPLC method is a reverse phase chromatographic method using phenomenex C(18) column and acetonitrile:phosphate buffer (65:35) pH adjusted to 5.75 with o-phosphoric acid as mobile phase and glipizide as internal standard. The linearity was observed in concentration range of 0-25 μg/ml for metformin hydrochloride. Results of analysis were validated statistically and by recovery studies.

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