HPLC Method for Estimation of Metformin Hydrochloride in Formulated Microspheres and Tablet Dosage Form

A simple, accurate, economical and reproducible HPLC method has been developed for quantitative estimation of metformin hydrochloride from tablet dosage form and formulated microspheres. The developed HPLC method is a reverse phase chromatographic method using phenomenex C(18) column and acetonitril...

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Detalles Bibliográficos
Autores principales: Kar, Mousumi, Choudhury, P. K.
Formato: Texto
Lenguaje:English
Publicado: Medknow Publications 2009
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2865795/
https://www.ncbi.nlm.nih.gov/pubmed/20490303
http://dx.doi.org/10.4103/0250-474X.56031
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author Kar, Mousumi
Choudhury, P. K.
author_facet Kar, Mousumi
Choudhury, P. K.
author_sort Kar, Mousumi
collection PubMed
description A simple, accurate, economical and reproducible HPLC method has been developed for quantitative estimation of metformin hydrochloride from tablet dosage form and formulated microspheres. The developed HPLC method is a reverse phase chromatographic method using phenomenex C(18) column and acetonitrile:phosphate buffer (65:35) pH adjusted to 5.75 with o-phosphoric acid as mobile phase and glipizide as internal standard. The linearity was observed in concentration range of 0-25 μg/ml for metformin hydrochloride. Results of analysis were validated statistically and by recovery studies.
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spelling pubmed-28657952010-05-20 HPLC Method for Estimation of Metformin Hydrochloride in Formulated Microspheres and Tablet Dosage Form Kar, Mousumi Choudhury, P. K. Indian J Pharm Sci Short Communication A simple, accurate, economical and reproducible HPLC method has been developed for quantitative estimation of metformin hydrochloride from tablet dosage form and formulated microspheres. The developed HPLC method is a reverse phase chromatographic method using phenomenex C(18) column and acetonitrile:phosphate buffer (65:35) pH adjusted to 5.75 with o-phosphoric acid as mobile phase and glipizide as internal standard. The linearity was observed in concentration range of 0-25 μg/ml for metformin hydrochloride. Results of analysis were validated statistically and by recovery studies. Medknow Publications 2009 /pmc/articles/PMC2865795/ /pubmed/20490303 http://dx.doi.org/10.4103/0250-474X.56031 Text en © Indian Journal of Pharmaceutical Sciences http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Kar, Mousumi
Choudhury, P. K.
HPLC Method for Estimation of Metformin Hydrochloride in Formulated Microspheres and Tablet Dosage Form
title HPLC Method for Estimation of Metformin Hydrochloride in Formulated Microspheres and Tablet Dosage Form
title_full HPLC Method for Estimation of Metformin Hydrochloride in Formulated Microspheres and Tablet Dosage Form
title_fullStr HPLC Method for Estimation of Metformin Hydrochloride in Formulated Microspheres and Tablet Dosage Form
title_full_unstemmed HPLC Method for Estimation of Metformin Hydrochloride in Formulated Microspheres and Tablet Dosage Form
title_short HPLC Method for Estimation of Metformin Hydrochloride in Formulated Microspheres and Tablet Dosage Form
title_sort hplc method for estimation of metformin hydrochloride in formulated microspheres and tablet dosage form
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2865795/
https://www.ncbi.nlm.nih.gov/pubmed/20490303
http://dx.doi.org/10.4103/0250-474X.56031
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