Cargando…

Interferon-gamma, interleukin-4 and interleukin-10 production by T helper cells reveals intact Th1 and regulatory T(R)1 cell activation and a delay of the Th2 cell response in equine neonates and foals

Cytokines produced by T helper (Th) cells are important in orchestrating the immune response during health and disease. Recent reports indicated that cytokine mRNA expression in foals is often quantitatively lower than that of adult horses suggesting that foal T cells are not fully mature. Here, per...

Descripción completa

Detalles Bibliográficos
Autores principales: Wagner, Bettina, Burton, Alexandra, Ainsworth, Dorothy
Formato: Texto
Lenguaje:English
Publicado: EDP Sciences 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2865874/
https://www.ncbi.nlm.nih.gov/pubmed/20374696
http://dx.doi.org/10.1051/vetres/2010019
_version_ 1782180882195415040
author Wagner, Bettina
Burton, Alexandra
Ainsworth, Dorothy
author_facet Wagner, Bettina
Burton, Alexandra
Ainsworth, Dorothy
author_sort Wagner, Bettina
collection PubMed
description Cytokines produced by T helper (Th) cells are important in orchestrating the immune response during health and disease. Recent reports indicated that cytokine mRNA expression in foals is often quantitatively lower than that of adult horses suggesting that foal T cells are not fully mature. Here, peripheral blood mononuclear cells from foals and adult horses were stimulated with phorbol 12-myristate 13-acetate and analyzed for intracellular interferon-gamma (IFN-γ), interleukin-4 (IL-4) and IL-10 production, representing the Th1, Th2 and regulatory T(R)1 cell phenotypes respectively, by flow cytometry. In agreement with previous reports, all three cytokines were quantitatively reduced in foals compared to adults. However, the balance between Th1 and Th2 cytokines (IFN-γ/IL-4 ratio) showed a clear Th1-biased response in foals by 6 and 12 weeks of life, while similar IFN-γ/IL-10 ratios were found in foals and adult horses. By day 5 after birth, intracellular IFN-γ production by foal CD4(+) and CD8(+) T cells resembled that in adults. Overall, IL-4 production was low in foals. IL-4(+) cells peaked at day 5 of age when IL-4 was mainly produced by IgE(+) cells. Relative percentages of IL-4(+) Th2 cells were significantly lower in foals at all time points. The data suggested that equine neonates and young foals have an impaired Th2 response, that the immune response of foals is Th1 biased, that IFN-γ production by Th and cytotoxic T cells is qualitatively similar to adult horses, and regulatory IL-10 production by T cells is developmentally mature in foals during the first three months of life.
format Text
id pubmed-2865874
institution National Center for Biotechnology Information
language English
publishDate 2010
publisher EDP Sciences
record_format MEDLINE/PubMed
spelling pubmed-28658742010-05-07 Interferon-gamma, interleukin-4 and interleukin-10 production by T helper cells reveals intact Th1 and regulatory T(R)1 cell activation and a delay of the Th2 cell response in equine neonates and foals Wagner, Bettina Burton, Alexandra Ainsworth, Dorothy Vet Res Original Article Cytokines produced by T helper (Th) cells are important in orchestrating the immune response during health and disease. Recent reports indicated that cytokine mRNA expression in foals is often quantitatively lower than that of adult horses suggesting that foal T cells are not fully mature. Here, peripheral blood mononuclear cells from foals and adult horses were stimulated with phorbol 12-myristate 13-acetate and analyzed for intracellular interferon-gamma (IFN-γ), interleukin-4 (IL-4) and IL-10 production, representing the Th1, Th2 and regulatory T(R)1 cell phenotypes respectively, by flow cytometry. In agreement with previous reports, all three cytokines were quantitatively reduced in foals compared to adults. However, the balance between Th1 and Th2 cytokines (IFN-γ/IL-4 ratio) showed a clear Th1-biased response in foals by 6 and 12 weeks of life, while similar IFN-γ/IL-10 ratios were found in foals and adult horses. By day 5 after birth, intracellular IFN-γ production by foal CD4(+) and CD8(+) T cells resembled that in adults. Overall, IL-4 production was low in foals. IL-4(+) cells peaked at day 5 of age when IL-4 was mainly produced by IgE(+) cells. Relative percentages of IL-4(+) Th2 cells were significantly lower in foals at all time points. The data suggested that equine neonates and young foals have an impaired Th2 response, that the immune response of foals is Th1 biased, that IFN-γ production by Th and cytotoxic T cells is qualitatively similar to adult horses, and regulatory IL-10 production by T cells is developmentally mature in foals during the first three months of life. EDP Sciences 2010-04-09 2010 /pmc/articles/PMC2865874/ /pubmed/20374696 http://dx.doi.org/10.1051/vetres/2010019 Text en © The authors, published by INRA/EDP Sciences, 2010 This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted use, distribution, and reproduction in any noncommercial medium, provided the original work is properly cited.
spellingShingle Original Article
Wagner, Bettina
Burton, Alexandra
Ainsworth, Dorothy
Interferon-gamma, interleukin-4 and interleukin-10 production by T helper cells reveals intact Th1 and regulatory T(R)1 cell activation and a delay of the Th2 cell response in equine neonates and foals
title Interferon-gamma, interleukin-4 and interleukin-10 production by T helper cells reveals intact Th1 and regulatory T(R)1 cell activation and a delay of the Th2 cell response in equine neonates and foals
title_full Interferon-gamma, interleukin-4 and interleukin-10 production by T helper cells reveals intact Th1 and regulatory T(R)1 cell activation and a delay of the Th2 cell response in equine neonates and foals
title_fullStr Interferon-gamma, interleukin-4 and interleukin-10 production by T helper cells reveals intact Th1 and regulatory T(R)1 cell activation and a delay of the Th2 cell response in equine neonates and foals
title_full_unstemmed Interferon-gamma, interleukin-4 and interleukin-10 production by T helper cells reveals intact Th1 and regulatory T(R)1 cell activation and a delay of the Th2 cell response in equine neonates and foals
title_short Interferon-gamma, interleukin-4 and interleukin-10 production by T helper cells reveals intact Th1 and regulatory T(R)1 cell activation and a delay of the Th2 cell response in equine neonates and foals
title_sort interferon-gamma, interleukin-4 and interleukin-10 production by t helper cells reveals intact th1 and regulatory t(r)1 cell activation and a delay of the th2 cell response in equine neonates and foals
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2865874/
https://www.ncbi.nlm.nih.gov/pubmed/20374696
http://dx.doi.org/10.1051/vetres/2010019
work_keys_str_mv AT wagnerbettina interferongammainterleukin4andinterleukin10productionbythelpercellsrevealsintactth1andregulatorytr1cellactivationandadelayoftheth2cellresponseinequineneonatesandfoals
AT burtonalexandra interferongammainterleukin4andinterleukin10productionbythelpercellsrevealsintactth1andregulatorytr1cellactivationandadelayoftheth2cellresponseinequineneonatesandfoals
AT ainsworthdorothy interferongammainterleukin4andinterleukin10productionbythelpercellsrevealsintactth1andregulatorytr1cellactivationandadelayoftheth2cellresponseinequineneonatesandfoals