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Cost-Effective Sequencing of Full-Length cDNA Clones Powered by a De Novo-Reference Hybrid Assembly

BACKGROUND: Sequencing full-length cDNA clones is important to determine gene structures including alternative splice forms, and provides valuable resources for experimental analyses to reveal the biological functions of coded proteins. However, previous approaches for sequencing cDNA clones were ex...

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Detalles Bibliográficos
Autores principales:	Kuroshu, Reginaldo M., Watanabe,, Junichi, Sugano, Sumio, Morishita, Shinichi, Suzuki, Yutaka, Kasahara, Masahiro
Formato:	Texto
Lenguaje:	English
Publicado:	Public Library of Science 2010
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2866332/ https://www.ncbi.nlm.nih.gov/pubmed/20479877 http://dx.doi.org/10.1371/journal.pone.0010517

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