Cargando…

Transplantation of canine umbilical cord blood-derived mesenchymal stem cells in experimentally induced spinal cord injured dogs

This study was to determine the effects of allogenic umbilical cord blood (UCB)-derived mesenchymal stem cells (MSCs) and recombinant methionyl human granulocyte colony-stimulating factor (rmhGCSF) on a canine spinal cord injury model after balloon compression at the first lumbar vertebra. Twenty-fi...

Descripción completa

Detalles Bibliográficos
Autores principales: Lim, Ji-Hey, Byeon, Ye-Eun, Ryu, Hak-Hyun, Jeong, Yun-Hyeok, Lee, Young-Won, Kim, Wan Hee, Kang, Kyung-Sun, Kweon, Oh-Kyeong
Formato: Texto
Lenguaje:English
Publicado: The Korean Society of Veterinary Science 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2868135/
https://www.ncbi.nlm.nih.gov/pubmed/17679775
http://dx.doi.org/10.4142/jvs.2007.8.3.275
_version_ 1782181040687677440
author Lim, Ji-Hey
Byeon, Ye-Eun
Ryu, Hak-Hyun
Jeong, Yun-Hyeok
Lee, Young-Won
Kim, Wan Hee
Kang, Kyung-Sun
Kweon, Oh-Kyeong
author_facet Lim, Ji-Hey
Byeon, Ye-Eun
Ryu, Hak-Hyun
Jeong, Yun-Hyeok
Lee, Young-Won
Kim, Wan Hee
Kang, Kyung-Sun
Kweon, Oh-Kyeong
author_sort Lim, Ji-Hey
collection PubMed
description This study was to determine the effects of allogenic umbilical cord blood (UCB)-derived mesenchymal stem cells (MSCs) and recombinant methionyl human granulocyte colony-stimulating factor (rmhGCSF) on a canine spinal cord injury model after balloon compression at the first lumbar vertebra. Twenty-five adult mongrel dogs were assigned to five groups according to treatment after a spinal cord injury: no treatment (CN); saline treatment (CP); rmhGCSF treatment (G); UCB-MSCs treatment (UCB-MSC); co-treatment (UCBG). The UCB-MSCs isolated from cord blood of canine fetuses were prepared as 10(6) cells/150 µl saline. The UCB-MSCs were directly injected into the injured site of the spinal cord and rmhGCSF was administered subcutaneously 1 week after the induction of spinal cord injury. The Olby score, magnetic resonance imaging, somatosensory evoked potentials and histopathological examinations were used to evaluate the functional recovery after transplantation. The Olby scores of all groups were zero at the 0-week evaluation. At 2 week after the transplantation, the Olby scores in the groups with the UCB-MSC and UCBG were significantly higher than in the CN and CP groups. However, there were no significant differences between the UCB-MSC and UCBG groups, and between the CN and CP groups. These comparisons remained stable at 4 and 8 week after transplantation. There was significant improvement in the nerve conduction velocity based on the somatosensory evoked potentials. In addition, a distinct structural consistency of the nerve cell bodies was noted in the lesion of the spinal cord of the UCB-MSC and UCBG groups. These results suggest that transplantation of the UCB-MSCs resulted in recovery of nerve function in dogs with a spinal cord injury and may be considered as a therapeutic modality for spinal cord injury.
format Text
id pubmed-2868135
institution National Center for Biotechnology Information
language English
publishDate 2007
publisher The Korean Society of Veterinary Science
record_format MEDLINE/PubMed
spelling pubmed-28681352010-05-13 Transplantation of canine umbilical cord blood-derived mesenchymal stem cells in experimentally induced spinal cord injured dogs Lim, Ji-Hey Byeon, Ye-Eun Ryu, Hak-Hyun Jeong, Yun-Hyeok Lee, Young-Won Kim, Wan Hee Kang, Kyung-Sun Kweon, Oh-Kyeong J Vet Sci Original Article This study was to determine the effects of allogenic umbilical cord blood (UCB)-derived mesenchymal stem cells (MSCs) and recombinant methionyl human granulocyte colony-stimulating factor (rmhGCSF) on a canine spinal cord injury model after balloon compression at the first lumbar vertebra. Twenty-five adult mongrel dogs were assigned to five groups according to treatment after a spinal cord injury: no treatment (CN); saline treatment (CP); rmhGCSF treatment (G); UCB-MSCs treatment (UCB-MSC); co-treatment (UCBG). The UCB-MSCs isolated from cord blood of canine fetuses were prepared as 10(6) cells/150 µl saline. The UCB-MSCs were directly injected into the injured site of the spinal cord and rmhGCSF was administered subcutaneously 1 week after the induction of spinal cord injury. The Olby score, magnetic resonance imaging, somatosensory evoked potentials and histopathological examinations were used to evaluate the functional recovery after transplantation. The Olby scores of all groups were zero at the 0-week evaluation. At 2 week after the transplantation, the Olby scores in the groups with the UCB-MSC and UCBG were significantly higher than in the CN and CP groups. However, there were no significant differences between the UCB-MSC and UCBG groups, and between the CN and CP groups. These comparisons remained stable at 4 and 8 week after transplantation. There was significant improvement in the nerve conduction velocity based on the somatosensory evoked potentials. In addition, a distinct structural consistency of the nerve cell bodies was noted in the lesion of the spinal cord of the UCB-MSC and UCBG groups. These results suggest that transplantation of the UCB-MSCs resulted in recovery of nerve function in dogs with a spinal cord injury and may be considered as a therapeutic modality for spinal cord injury. The Korean Society of Veterinary Science 2007-09 2007-09-30 /pmc/articles/PMC2868135/ /pubmed/17679775 http://dx.doi.org/10.4142/jvs.2007.8.3.275 Text en Copyright © 2007 The Korean Society of Veterinary Science https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Lim, Ji-Hey
Byeon, Ye-Eun
Ryu, Hak-Hyun
Jeong, Yun-Hyeok
Lee, Young-Won
Kim, Wan Hee
Kang, Kyung-Sun
Kweon, Oh-Kyeong
Transplantation of canine umbilical cord blood-derived mesenchymal stem cells in experimentally induced spinal cord injured dogs
title Transplantation of canine umbilical cord blood-derived mesenchymal stem cells in experimentally induced spinal cord injured dogs
title_full Transplantation of canine umbilical cord blood-derived mesenchymal stem cells in experimentally induced spinal cord injured dogs
title_fullStr Transplantation of canine umbilical cord blood-derived mesenchymal stem cells in experimentally induced spinal cord injured dogs
title_full_unstemmed Transplantation of canine umbilical cord blood-derived mesenchymal stem cells in experimentally induced spinal cord injured dogs
title_short Transplantation of canine umbilical cord blood-derived mesenchymal stem cells in experimentally induced spinal cord injured dogs
title_sort transplantation of canine umbilical cord blood-derived mesenchymal stem cells in experimentally induced spinal cord injured dogs
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2868135/
https://www.ncbi.nlm.nih.gov/pubmed/17679775
http://dx.doi.org/10.4142/jvs.2007.8.3.275
work_keys_str_mv AT limjihey transplantationofcanineumbilicalcordbloodderivedmesenchymalstemcellsinexperimentallyinducedspinalcordinjureddogs
AT byeonyeeun transplantationofcanineumbilicalcordbloodderivedmesenchymalstemcellsinexperimentallyinducedspinalcordinjureddogs
AT ryuhakhyun transplantationofcanineumbilicalcordbloodderivedmesenchymalstemcellsinexperimentallyinducedspinalcordinjureddogs
AT jeongyunhyeok transplantationofcanineumbilicalcordbloodderivedmesenchymalstemcellsinexperimentallyinducedspinalcordinjureddogs
AT leeyoungwon transplantationofcanineumbilicalcordbloodderivedmesenchymalstemcellsinexperimentallyinducedspinalcordinjureddogs
AT kimwanhee transplantationofcanineumbilicalcordbloodderivedmesenchymalstemcellsinexperimentallyinducedspinalcordinjureddogs
AT kangkyungsun transplantationofcanineumbilicalcordbloodderivedmesenchymalstemcellsinexperimentallyinducedspinalcordinjureddogs
AT kweonohkyeong transplantationofcanineumbilicalcordbloodderivedmesenchymalstemcellsinexperimentallyinducedspinalcordinjureddogs