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1,9-Bis(2-pyridyl)-1,2,8,9-tetrathia-5-oxanonane

Disulfide crosslinking of proteins is typically performed by treating proteins bearing cysteine residues with small-molecule disulfide reagents. The process results in the formation of a mixed disulfide intermediate, which then reacts with the cysteine residue of another protein molecule to form the...

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Detalles Bibliográficos
Autores principales: Kalia, Jeet, Raines, Ronald T.
Formato: Texto
Lenguaje:English
Publicado: 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2868194/
https://www.ncbi.nlm.nih.gov/pubmed/20467570
http://dx.doi.org/10.3390/M642
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author Kalia, Jeet
Raines, Ronald T.
author_facet Kalia, Jeet
Raines, Ronald T.
author_sort Kalia, Jeet
collection PubMed
description Disulfide crosslinking of proteins is typically performed by treating proteins bearing cysteine residues with small-molecule disulfide reagents. The process results in the formation of a mixed disulfide intermediate, which then reacts with the cysteine residue of another protein molecule to form the crosslinked product. This second step requires the intimate association of two large reactants. The ensuing steric hindrance can result in poor crosslinking yields. Here, we introduce a bis(disulfide) reagent in which activated disulfides are separated by linkers that can alleviate steric hindrance and thereby potentially increase the efficiency of crosslinking.
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spelling pubmed-28681942010-05-12 1,9-Bis(2-pyridyl)-1,2,8,9-tetrathia-5-oxanonane Kalia, Jeet Raines, Ronald T. Molbank Article Disulfide crosslinking of proteins is typically performed by treating proteins bearing cysteine residues with small-molecule disulfide reagents. The process results in the formation of a mixed disulfide intermediate, which then reacts with the cysteine residue of another protein molecule to form the crosslinked product. This second step requires the intimate association of two large reactants. The ensuing steric hindrance can result in poor crosslinking yields. Here, we introduce a bis(disulfide) reagent in which activated disulfides are separated by linkers that can alleviate steric hindrance and thereby potentially increase the efficiency of crosslinking. 2009 /pmc/articles/PMC2868194/ /pubmed/20467570 http://dx.doi.org/10.3390/M642 Text en © 2009 by the authors licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Kalia, Jeet
Raines, Ronald T.
1,9-Bis(2-pyridyl)-1,2,8,9-tetrathia-5-oxanonane
title 1,9-Bis(2-pyridyl)-1,2,8,9-tetrathia-5-oxanonane
title_full 1,9-Bis(2-pyridyl)-1,2,8,9-tetrathia-5-oxanonane
title_fullStr 1,9-Bis(2-pyridyl)-1,2,8,9-tetrathia-5-oxanonane
title_full_unstemmed 1,9-Bis(2-pyridyl)-1,2,8,9-tetrathia-5-oxanonane
title_short 1,9-Bis(2-pyridyl)-1,2,8,9-tetrathia-5-oxanonane
title_sort 1,9-bis(2-pyridyl)-1,2,8,9-tetrathia-5-oxanonane
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2868194/
https://www.ncbi.nlm.nih.gov/pubmed/20467570
http://dx.doi.org/10.3390/M642
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