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1,9-Bis(2-pyridyl)-1,2,8,9-tetrathia-5-oxanonane
Disulfide crosslinking of proteins is typically performed by treating proteins bearing cysteine residues with small-molecule disulfide reagents. The process results in the formation of a mixed disulfide intermediate, which then reacts with the cysteine residue of another protein molecule to form the...
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Formato: | Texto |
Lenguaje: | English |
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2009
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2868194/ https://www.ncbi.nlm.nih.gov/pubmed/20467570 http://dx.doi.org/10.3390/M642 |
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author | Kalia, Jeet Raines, Ronald T. |
author_facet | Kalia, Jeet Raines, Ronald T. |
author_sort | Kalia, Jeet |
collection | PubMed |
description | Disulfide crosslinking of proteins is typically performed by treating proteins bearing cysteine residues with small-molecule disulfide reagents. The process results in the formation of a mixed disulfide intermediate, which then reacts with the cysteine residue of another protein molecule to form the crosslinked product. This second step requires the intimate association of two large reactants. The ensuing steric hindrance can result in poor crosslinking yields. Here, we introduce a bis(disulfide) reagent in which activated disulfides are separated by linkers that can alleviate steric hindrance and thereby potentially increase the efficiency of crosslinking. |
format | Text |
id | pubmed-2868194 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
record_format | MEDLINE/PubMed |
spelling | pubmed-28681942010-05-12 1,9-Bis(2-pyridyl)-1,2,8,9-tetrathia-5-oxanonane Kalia, Jeet Raines, Ronald T. Molbank Article Disulfide crosslinking of proteins is typically performed by treating proteins bearing cysteine residues with small-molecule disulfide reagents. The process results in the formation of a mixed disulfide intermediate, which then reacts with the cysteine residue of another protein molecule to form the crosslinked product. This second step requires the intimate association of two large reactants. The ensuing steric hindrance can result in poor crosslinking yields. Here, we introduce a bis(disulfide) reagent in which activated disulfides are separated by linkers that can alleviate steric hindrance and thereby potentially increase the efficiency of crosslinking. 2009 /pmc/articles/PMC2868194/ /pubmed/20467570 http://dx.doi.org/10.3390/M642 Text en © 2009 by the authors licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Kalia, Jeet Raines, Ronald T. 1,9-Bis(2-pyridyl)-1,2,8,9-tetrathia-5-oxanonane |
title | 1,9-Bis(2-pyridyl)-1,2,8,9-tetrathia-5-oxanonane |
title_full | 1,9-Bis(2-pyridyl)-1,2,8,9-tetrathia-5-oxanonane |
title_fullStr | 1,9-Bis(2-pyridyl)-1,2,8,9-tetrathia-5-oxanonane |
title_full_unstemmed | 1,9-Bis(2-pyridyl)-1,2,8,9-tetrathia-5-oxanonane |
title_short | 1,9-Bis(2-pyridyl)-1,2,8,9-tetrathia-5-oxanonane |
title_sort | 1,9-bis(2-pyridyl)-1,2,8,9-tetrathia-5-oxanonane |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2868194/ https://www.ncbi.nlm.nih.gov/pubmed/20467570 http://dx.doi.org/10.3390/M642 |
work_keys_str_mv | AT kaliajeet 19bis2pyridyl1289tetrathia5oxanonane AT rainesronaldt 19bis2pyridyl1289tetrathia5oxanonane |