Cargando…
Brucella abortus ure2 region contains an acid-activated urea transporter and a nickel transport system
BACKGROUND: Urease is a virulence factor that plays a role in the resistance of Brucella to low pH conditions, both in vivo and in vitro. Brucella contains two separate urease gene clusters, ure1 and ure2. Although only ure1 codes for an active urease, ure2 is also transcribed, but its contribution...
Autores principales: | , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2010
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2868824/ https://www.ncbi.nlm.nih.gov/pubmed/20380737 http://dx.doi.org/10.1186/1471-2180-10-107 |
_version_ | 1782181070074019840 |
---|---|
author | Sangari, Félix J Cayón, Ana M Seoane, Asunción García-Lobo, Juan M |
author_facet | Sangari, Félix J Cayón, Ana M Seoane, Asunción García-Lobo, Juan M |
author_sort | Sangari, Félix J |
collection | PubMed |
description | BACKGROUND: Urease is a virulence factor that plays a role in the resistance of Brucella to low pH conditions, both in vivo and in vitro. Brucella contains two separate urease gene clusters, ure1 and ure2. Although only ure1 codes for an active urease, ure2 is also transcribed, but its contribution to Brucella biology is unknown. RESULTS: Re-examination of the ure2 locus showed that the operon includes five genes downstream of ureABCEFGDT that are orthologs to a nikKMLQO cluster encoding an ECF-type transport system for nickel. ureT and nikO mutants were constructed and analyzed for urease activity and acid resistance. A non-polar ureT mutant was unaffected in urease activity at neutral pH but showed a significantly decreased activity at acidic pH. It also showed a decreased survival rate to pH 2 at low concentration of urea when compared to the wild type. The nikO mutant had decreased urease activity and acid resistance at all urea concentrations tested, and this phenotype could be reverted by the addition of nickel to the growth medium. CONCLUSIONS: Based on these results, we concluded that the operon ure2 codes for an acid-activated urea transporter and a nickel transporter necessary for the maximal activity of the urease whose structural subunits are encoded exclusively by the genes in the ure1 operon. |
format | Text |
id | pubmed-2868824 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-28688242010-05-13 Brucella abortus ure2 region contains an acid-activated urea transporter and a nickel transport system Sangari, Félix J Cayón, Ana M Seoane, Asunción García-Lobo, Juan M BMC Microbiol Research article BACKGROUND: Urease is a virulence factor that plays a role in the resistance of Brucella to low pH conditions, both in vivo and in vitro. Brucella contains two separate urease gene clusters, ure1 and ure2. Although only ure1 codes for an active urease, ure2 is also transcribed, but its contribution to Brucella biology is unknown. RESULTS: Re-examination of the ure2 locus showed that the operon includes five genes downstream of ureABCEFGDT that are orthologs to a nikKMLQO cluster encoding an ECF-type transport system for nickel. ureT and nikO mutants were constructed and analyzed for urease activity and acid resistance. A non-polar ureT mutant was unaffected in urease activity at neutral pH but showed a significantly decreased activity at acidic pH. It also showed a decreased survival rate to pH 2 at low concentration of urea when compared to the wild type. The nikO mutant had decreased urease activity and acid resistance at all urea concentrations tested, and this phenotype could be reverted by the addition of nickel to the growth medium. CONCLUSIONS: Based on these results, we concluded that the operon ure2 codes for an acid-activated urea transporter and a nickel transporter necessary for the maximal activity of the urease whose structural subunits are encoded exclusively by the genes in the ure1 operon. BioMed Central 2010-04-10 /pmc/articles/PMC2868824/ /pubmed/20380737 http://dx.doi.org/10.1186/1471-2180-10-107 Text en Copyright ©2010 Sangari et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research article Sangari, Félix J Cayón, Ana M Seoane, Asunción García-Lobo, Juan M Brucella abortus ure2 region contains an acid-activated urea transporter and a nickel transport system |
title | Brucella abortus ure2 region contains an acid-activated urea transporter and a nickel transport system |
title_full | Brucella abortus ure2 region contains an acid-activated urea transporter and a nickel transport system |
title_fullStr | Brucella abortus ure2 region contains an acid-activated urea transporter and a nickel transport system |
title_full_unstemmed | Brucella abortus ure2 region contains an acid-activated urea transporter and a nickel transport system |
title_short | Brucella abortus ure2 region contains an acid-activated urea transporter and a nickel transport system |
title_sort | brucella abortus ure2 region contains an acid-activated urea transporter and a nickel transport system |
topic | Research article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2868824/ https://www.ncbi.nlm.nih.gov/pubmed/20380737 http://dx.doi.org/10.1186/1471-2180-10-107 |
work_keys_str_mv | AT sangarifelixj brucellaabortusure2regioncontainsanacidactivatedureatransporterandanickeltransportsystem AT cayonanam brucellaabortusure2regioncontainsanacidactivatedureatransporterandanickeltransportsystem AT seoaneasuncion brucellaabortusure2regioncontainsanacidactivatedureatransporterandanickeltransportsystem AT garcialobojuanm brucellaabortusure2regioncontainsanacidactivatedureatransporterandanickeltransportsystem |