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Microvessel changes after post-ischemic benign and malignant hyperemia: experimental study in rats
BACKGROUND: The present investigation was designed to elucidate the use of dynamic contrast enhanced perfusion MR imaging (DCE pMRI) in characterizing hyperemia, including microvessel changes, and to examine whether DCE pMRI can predict benign or malignant hyperemia. METHODS: Sprague-Dawley rats und...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2868835/ https://www.ncbi.nlm.nih.gov/pubmed/20398382 http://dx.doi.org/10.1186/1471-2377-10-24 |
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author | Lu, Haitao Zhao, Jungong Li, Minghua Cheng, Yingsheng Li, Yongdong You, Xiaofang Zhao, Yuwu |
author_facet | Lu, Haitao Zhao, Jungong Li, Minghua Cheng, Yingsheng Li, Yongdong You, Xiaofang Zhao, Yuwu |
author_sort | Lu, Haitao |
collection | PubMed |
description | BACKGROUND: The present investigation was designed to elucidate the use of dynamic contrast enhanced perfusion MR imaging (DCE pMRI) in characterizing hyperemia, including microvessel changes, and to examine whether DCE pMRI can predict benign or malignant hyperemia. METHODS: Sprague-Dawley rats underwent middle cerebral artery occlusion (MCAO) by intraluminal suture placement. All rats were randomized to 4 groups: MCAO for 0.5 hours followed by saline treatment (10 ml/kg; group 1); MCAO for 3 hours followed by treatment with saline (group 2) or urokinase (25000 IU/kg; group 3); and MCAO for 6 hours followed by urokinase treatment (group 4). Relative cerebral blood volume (rCBV) and relative maximum slope of increase of the signal intensity time curve (rMSI) were quantitatively analyzed from MRI. Microvessel diameter and blood-brain barrier disruption obtained by laser scanning confocal microscopy (LSCM) as well as transmission electron microscopy (TEM) were obtained for correlative study. RESULTS: Benign hyperemia was noticed only in group 1; malignant hyperemia was seen in group 3. Although the rCBV of malignant hyperemia was slightly higher than in benign hyperemia (P > 0.05), the rMSI, on the other hand, was significantly lower (P < 0.05). Fluoro-isothiocyanate dextran (FITC-dextran) extravasations, marked glial end-foot process swelling, and significant vasodilatation were seen in malignant hyperemia, while no or mild leakage of FITC-dextran and slight glial end-foot process swelling occurred in benign hyperemia. CONCLUSION: Our findings indicate that DCE pMRI can characterize post-ischemic hyperemia and correlates well with microvascular damage. |
format | Text |
id | pubmed-2868835 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-28688352010-05-13 Microvessel changes after post-ischemic benign and malignant hyperemia: experimental study in rats Lu, Haitao Zhao, Jungong Li, Minghua Cheng, Yingsheng Li, Yongdong You, Xiaofang Zhao, Yuwu BMC Neurol Research article BACKGROUND: The present investigation was designed to elucidate the use of dynamic contrast enhanced perfusion MR imaging (DCE pMRI) in characterizing hyperemia, including microvessel changes, and to examine whether DCE pMRI can predict benign or malignant hyperemia. METHODS: Sprague-Dawley rats underwent middle cerebral artery occlusion (MCAO) by intraluminal suture placement. All rats were randomized to 4 groups: MCAO for 0.5 hours followed by saline treatment (10 ml/kg; group 1); MCAO for 3 hours followed by treatment with saline (group 2) or urokinase (25000 IU/kg; group 3); and MCAO for 6 hours followed by urokinase treatment (group 4). Relative cerebral blood volume (rCBV) and relative maximum slope of increase of the signal intensity time curve (rMSI) were quantitatively analyzed from MRI. Microvessel diameter and blood-brain barrier disruption obtained by laser scanning confocal microscopy (LSCM) as well as transmission electron microscopy (TEM) were obtained for correlative study. RESULTS: Benign hyperemia was noticed only in group 1; malignant hyperemia was seen in group 3. Although the rCBV of malignant hyperemia was slightly higher than in benign hyperemia (P > 0.05), the rMSI, on the other hand, was significantly lower (P < 0.05). Fluoro-isothiocyanate dextran (FITC-dextran) extravasations, marked glial end-foot process swelling, and significant vasodilatation were seen in malignant hyperemia, while no or mild leakage of FITC-dextran and slight glial end-foot process swelling occurred in benign hyperemia. CONCLUSION: Our findings indicate that DCE pMRI can characterize post-ischemic hyperemia and correlates well with microvascular damage. BioMed Central 2010-04-16 /pmc/articles/PMC2868835/ /pubmed/20398382 http://dx.doi.org/10.1186/1471-2377-10-24 Text en Copyright ©2010 Lu et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research article Lu, Haitao Zhao, Jungong Li, Minghua Cheng, Yingsheng Li, Yongdong You, Xiaofang Zhao, Yuwu Microvessel changes after post-ischemic benign and malignant hyperemia: experimental study in rats |
title | Microvessel changes after post-ischemic benign and malignant hyperemia: experimental study in rats |
title_full | Microvessel changes after post-ischemic benign and malignant hyperemia: experimental study in rats |
title_fullStr | Microvessel changes after post-ischemic benign and malignant hyperemia: experimental study in rats |
title_full_unstemmed | Microvessel changes after post-ischemic benign and malignant hyperemia: experimental study in rats |
title_short | Microvessel changes after post-ischemic benign and malignant hyperemia: experimental study in rats |
title_sort | microvessel changes after post-ischemic benign and malignant hyperemia: experimental study in rats |
topic | Research article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2868835/ https://www.ncbi.nlm.nih.gov/pubmed/20398382 http://dx.doi.org/10.1186/1471-2377-10-24 |
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