Cargando…
Activation of the Mitogen-activated Protein Kinase, Slt2p, at Bud Tips Blocks a Late Stage of Endoplasmic Reticulum Inheritance in Saccharomyces cerevisiae
Inheritance of the endoplasmic reticulum (ER) requires Ptc1p, a type 2C protein phosphatase of Saccharomyces cerevisiae. Genetic analysis indicates that Ptc1p is needed to inactivate the cell wall integrity (CWI) MAP kinase, Slt2p. Here we show that under normal growth conditions, Ptc1p inactivates...
Autores principales: | , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2010
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2869382/ https://www.ncbi.nlm.nih.gov/pubmed/20357006 http://dx.doi.org/10.1091/mbc.E09-06-0532 |
_version_ | 1782181124996333568 |
---|---|
author | Li, Xia Du, Yunrui Siegel, Steven Ferro-Novick, Susan Novick, Peter |
author_facet | Li, Xia Du, Yunrui Siegel, Steven Ferro-Novick, Susan Novick, Peter |
author_sort | Li, Xia |
collection | PubMed |
description | Inheritance of the endoplasmic reticulum (ER) requires Ptc1p, a type 2C protein phosphatase of Saccharomyces cerevisiae. Genetic analysis indicates that Ptc1p is needed to inactivate the cell wall integrity (CWI) MAP kinase, Slt2p. Here we show that under normal growth conditions, Ptc1p inactivates Slt2p just as ER tubules begin to spread from the bud tip along the cortex. In ptc1Δ cells, the propagation of cortical ER from the bud tip to the periphery of the bud is delayed by hyperactivation of Slt2p. The pool of Slt2p that controls ER inheritance requires the CWI pathway scaffold, Spa2p, for its retention at the bud tip, and a mutation within Slt2p that prevents its association with the bud tip blocks its role in ER inheritance. These results imply that Slt2p inhibits a late step in ER inheritance by phosphorylating a target at the tip of daughter cells. The PI4P5-kinase, Mss4p, is an upstream activator of this pool of Slt2p. Ptc1p-dependant inactivation of Slt2p is also needed for mitochondrial inheritance; however, in this case, the relevant pool of Slt2p is not at the bud tip. |
format | Text |
id | pubmed-2869382 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-28693822010-07-30 Activation of the Mitogen-activated Protein Kinase, Slt2p, at Bud Tips Blocks a Late Stage of Endoplasmic Reticulum Inheritance in Saccharomyces cerevisiae Li, Xia Du, Yunrui Siegel, Steven Ferro-Novick, Susan Novick, Peter Mol Biol Cell Articles Inheritance of the endoplasmic reticulum (ER) requires Ptc1p, a type 2C protein phosphatase of Saccharomyces cerevisiae. Genetic analysis indicates that Ptc1p is needed to inactivate the cell wall integrity (CWI) MAP kinase, Slt2p. Here we show that under normal growth conditions, Ptc1p inactivates Slt2p just as ER tubules begin to spread from the bud tip along the cortex. In ptc1Δ cells, the propagation of cortical ER from the bud tip to the periphery of the bud is delayed by hyperactivation of Slt2p. The pool of Slt2p that controls ER inheritance requires the CWI pathway scaffold, Spa2p, for its retention at the bud tip, and a mutation within Slt2p that prevents its association with the bud tip blocks its role in ER inheritance. These results imply that Slt2p inhibits a late step in ER inheritance by phosphorylating a target at the tip of daughter cells. The PI4P5-kinase, Mss4p, is an upstream activator of this pool of Slt2p. Ptc1p-dependant inactivation of Slt2p is also needed for mitochondrial inheritance; however, in this case, the relevant pool of Slt2p is not at the bud tip. The American Society for Cell Biology 2010-05-15 /pmc/articles/PMC2869382/ /pubmed/20357006 http://dx.doi.org/10.1091/mbc.E09-06-0532 Text en © 2010 by The American Society for Cell Biology |
spellingShingle | Articles Li, Xia Du, Yunrui Siegel, Steven Ferro-Novick, Susan Novick, Peter Activation of the Mitogen-activated Protein Kinase, Slt2p, at Bud Tips Blocks a Late Stage of Endoplasmic Reticulum Inheritance in Saccharomyces cerevisiae |
title | Activation of the Mitogen-activated Protein Kinase, Slt2p, at Bud Tips Blocks a Late Stage of Endoplasmic Reticulum Inheritance in Saccharomyces cerevisiae |
title_full | Activation of the Mitogen-activated Protein Kinase, Slt2p, at Bud Tips Blocks a Late Stage of Endoplasmic Reticulum Inheritance in Saccharomyces cerevisiae |
title_fullStr | Activation of the Mitogen-activated Protein Kinase, Slt2p, at Bud Tips Blocks a Late Stage of Endoplasmic Reticulum Inheritance in Saccharomyces cerevisiae |
title_full_unstemmed | Activation of the Mitogen-activated Protein Kinase, Slt2p, at Bud Tips Blocks a Late Stage of Endoplasmic Reticulum Inheritance in Saccharomyces cerevisiae |
title_short | Activation of the Mitogen-activated Protein Kinase, Slt2p, at Bud Tips Blocks a Late Stage of Endoplasmic Reticulum Inheritance in Saccharomyces cerevisiae |
title_sort | activation of the mitogen-activated protein kinase, slt2p, at bud tips blocks a late stage of endoplasmic reticulum inheritance in saccharomyces cerevisiae |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2869382/ https://www.ncbi.nlm.nih.gov/pubmed/20357006 http://dx.doi.org/10.1091/mbc.E09-06-0532 |
work_keys_str_mv | AT lixia activationofthemitogenactivatedproteinkinaseslt2patbudtipsblocksalatestageofendoplasmicreticuluminheritanceinsaccharomycescerevisiae AT duyunrui activationofthemitogenactivatedproteinkinaseslt2patbudtipsblocksalatestageofendoplasmicreticuluminheritanceinsaccharomycescerevisiae AT siegelsteven activationofthemitogenactivatedproteinkinaseslt2patbudtipsblocksalatestageofendoplasmicreticuluminheritanceinsaccharomycescerevisiae AT ferronovicksusan activationofthemitogenactivatedproteinkinaseslt2patbudtipsblocksalatestageofendoplasmicreticuluminheritanceinsaccharomycescerevisiae AT novickpeter activationofthemitogenactivatedproteinkinaseslt2patbudtipsblocksalatestageofendoplasmicreticuluminheritanceinsaccharomycescerevisiae |