Titanium Immobilized with an Antimicrobial Peptide Derived from Histatin Accelerates the Differentiation of Osteoblastic Cell Line, MC3T3-E1

The objective of this study was to evaluate the effect of titanium immobilized with a cationic antimicrobial peptide (JH8194) derived from histatin on the biofilm formation of Porphyromonas gingivalis and differentiation of osteoblastic cells (MC3T3-E1). The titanium specimens (Ti) were immobilized...

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Autores principales: Makihira, Seicho, Shuto, Takahiro, Nikawa, Hiroki, Okamoto, Keishi, Mine, Yuichi, Takamoto, Yuko, Ohara, Masaru, Tsuji, Koichiro
Formato: Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2871126/
https://www.ncbi.nlm.nih.gov/pubmed/20480030
http://dx.doi.org/10.3390/ijms11041458
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author Makihira, Seicho
Shuto, Takahiro
Nikawa, Hiroki
Okamoto, Keishi
Mine, Yuichi
Takamoto, Yuko
Ohara, Masaru
Tsuji, Koichiro
author_facet Makihira, Seicho
Shuto, Takahiro
Nikawa, Hiroki
Okamoto, Keishi
Mine, Yuichi
Takamoto, Yuko
Ohara, Masaru
Tsuji, Koichiro
author_sort Makihira, Seicho
collection PubMed
description The objective of this study was to evaluate the effect of titanium immobilized with a cationic antimicrobial peptide (JH8194) derived from histatin on the biofilm formation of Porphyromonas gingivalis and differentiation of osteoblastic cells (MC3T3-E1). The titanium specimens (Ti) were immobilized with JH8194, according to the method previously described. The colonization of P. gingivalis on JH8194-Ti was significantly lower than that on control- and blocking-Ti. JH8194-Ti enhanced the mRNA expressions of Runx2 and OPN, and ALPase activity in the MC3T3-E1, as compared with those of control- and blocking-Ti. These results, taken together, suggested the possibility that JH8194-Ti may be a potential aid to shorten the period of acquiring osseointegration.
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spelling pubmed-28711262010-05-17 Titanium Immobilized with an Antimicrobial Peptide Derived from Histatin Accelerates the Differentiation of Osteoblastic Cell Line, MC3T3-E1 Makihira, Seicho Shuto, Takahiro Nikawa, Hiroki Okamoto, Keishi Mine, Yuichi Takamoto, Yuko Ohara, Masaru Tsuji, Koichiro Int J Mol Sci Article The objective of this study was to evaluate the effect of titanium immobilized with a cationic antimicrobial peptide (JH8194) derived from histatin on the biofilm formation of Porphyromonas gingivalis and differentiation of osteoblastic cells (MC3T3-E1). The titanium specimens (Ti) were immobilized with JH8194, according to the method previously described. The colonization of P. gingivalis on JH8194-Ti was significantly lower than that on control- and blocking-Ti. JH8194-Ti enhanced the mRNA expressions of Runx2 and OPN, and ALPase activity in the MC3T3-E1, as compared with those of control- and blocking-Ti. These results, taken together, suggested the possibility that JH8194-Ti may be a potential aid to shorten the period of acquiring osseointegration. Molecular Diversity Preservation International (MDPI) 2010-04-02 /pmc/articles/PMC2871126/ /pubmed/20480030 http://dx.doi.org/10.3390/ijms11041458 Text en © 2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Makihira, Seicho
Shuto, Takahiro
Nikawa, Hiroki
Okamoto, Keishi
Mine, Yuichi
Takamoto, Yuko
Ohara, Masaru
Tsuji, Koichiro
Titanium Immobilized with an Antimicrobial Peptide Derived from Histatin Accelerates the Differentiation of Osteoblastic Cell Line, MC3T3-E1
title Titanium Immobilized with an Antimicrobial Peptide Derived from Histatin Accelerates the Differentiation of Osteoblastic Cell Line, MC3T3-E1
title_full Titanium Immobilized with an Antimicrobial Peptide Derived from Histatin Accelerates the Differentiation of Osteoblastic Cell Line, MC3T3-E1
title_fullStr Titanium Immobilized with an Antimicrobial Peptide Derived from Histatin Accelerates the Differentiation of Osteoblastic Cell Line, MC3T3-E1
title_full_unstemmed Titanium Immobilized with an Antimicrobial Peptide Derived from Histatin Accelerates the Differentiation of Osteoblastic Cell Line, MC3T3-E1
title_short Titanium Immobilized with an Antimicrobial Peptide Derived from Histatin Accelerates the Differentiation of Osteoblastic Cell Line, MC3T3-E1
title_sort titanium immobilized with an antimicrobial peptide derived from histatin accelerates the differentiation of osteoblastic cell line, mc3t3-e1
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2871126/
https://www.ncbi.nlm.nih.gov/pubmed/20480030
http://dx.doi.org/10.3390/ijms11041458
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