Effects of Aflatoxin B(1) and Fumonisin B(1) on the Viability and Induction of Apoptosis in Rat Primary Hepatocytes

The present study evaluated the effect of aflatoxin B(1) (AFB(1)) and fumonisin B(1) (FB(1)) either alone, or in association, on rat primary hepatocyte cultures. Cell viability was assessed by flow cytometry after propidium iodine intercalation. DNA fragmentation and apoptosis were assessed by agaro...

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Detalles Bibliográficos
Autores principales: Ribeiro, Deise H. B., Ferreira, Fabiane L., da Silva, Valéria N., Aquino, Simone, Corrêa, Benedito
Formato: Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2871147/
https://www.ncbi.nlm.nih.gov/pubmed/20480051
http://dx.doi.org/10.3390/ijms11041944
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author Ribeiro, Deise H. B.
Ferreira, Fabiane L.
da Silva, Valéria N.
Aquino, Simone
Corrêa, Benedito
author_facet Ribeiro, Deise H. B.
Ferreira, Fabiane L.
da Silva, Valéria N.
Aquino, Simone
Corrêa, Benedito
author_sort Ribeiro, Deise H. B.
collection PubMed
description The present study evaluated the effect of aflatoxin B(1) (AFB(1)) and fumonisin B(1) (FB(1)) either alone, or in association, on rat primary hepatocyte cultures. Cell viability was assessed by flow cytometry after propidium iodine intercalation. DNA fragmentation and apoptosis were assessed by agarose gel electrophoresis and acridine orange and ethidium bromide staining. At the concentrations of AFB(1) and FB(1) used, the toxins did not decrease cell viability, but did induce apoptosis in a concentration and time-dependent manner.
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spelling pubmed-28711472010-05-17 Effects of Aflatoxin B(1) and Fumonisin B(1) on the Viability and Induction of Apoptosis in Rat Primary Hepatocytes Ribeiro, Deise H. B. Ferreira, Fabiane L. da Silva, Valéria N. Aquino, Simone Corrêa, Benedito Int J Mol Sci Article The present study evaluated the effect of aflatoxin B(1) (AFB(1)) and fumonisin B(1) (FB(1)) either alone, or in association, on rat primary hepatocyte cultures. Cell viability was assessed by flow cytometry after propidium iodine intercalation. DNA fragmentation and apoptosis were assessed by agarose gel electrophoresis and acridine orange and ethidium bromide staining. At the concentrations of AFB(1) and FB(1) used, the toxins did not decrease cell viability, but did induce apoptosis in a concentration and time-dependent manner. Molecular Diversity Preservation International (MDPI) 2010-04-26 /pmc/articles/PMC2871147/ /pubmed/20480051 http://dx.doi.org/10.3390/ijms11041944 Text en © 2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Ribeiro, Deise H. B.
Ferreira, Fabiane L.
da Silva, Valéria N.
Aquino, Simone
Corrêa, Benedito
Effects of Aflatoxin B(1) and Fumonisin B(1) on the Viability and Induction of Apoptosis in Rat Primary Hepatocytes
title Effects of Aflatoxin B(1) and Fumonisin B(1) on the Viability and Induction of Apoptosis in Rat Primary Hepatocytes
title_full Effects of Aflatoxin B(1) and Fumonisin B(1) on the Viability and Induction of Apoptosis in Rat Primary Hepatocytes
title_fullStr Effects of Aflatoxin B(1) and Fumonisin B(1) on the Viability and Induction of Apoptosis in Rat Primary Hepatocytes
title_full_unstemmed Effects of Aflatoxin B(1) and Fumonisin B(1) on the Viability and Induction of Apoptosis in Rat Primary Hepatocytes
title_short Effects of Aflatoxin B(1) and Fumonisin B(1) on the Viability and Induction of Apoptosis in Rat Primary Hepatocytes
title_sort effects of aflatoxin b(1) and fumonisin b(1) on the viability and induction of apoptosis in rat primary hepatocytes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2871147/
https://www.ncbi.nlm.nih.gov/pubmed/20480051
http://dx.doi.org/10.3390/ijms11041944
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