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Cell-associated hemolysis activity in the clinical strain of Pseudomonas fluorescens MFN1032

BACKGROUND: MFN1032 is a clinical Pseudomonas fluorescens strain able to grow at 37°C. MFN1032 cells induce necrosis and apoptosis in rat glial cells at this temperature. This strain displays secretion-mediated hemolytic activity involving phospholipase C and cyclolipopeptides. Under laboratory cond...

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Autores principales: Sperandio, Daniel, Rossignol, Gaelle, Guerillon, Josette, Connil, Nathalie, Orange, Nicole, Feuilloley, Marc GJ, Merieau, Annabelle
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2871272/
https://www.ncbi.nlm.nih.gov/pubmed/20416103
http://dx.doi.org/10.1186/1471-2180-10-124
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author Sperandio, Daniel
Rossignol, Gaelle
Guerillon, Josette
Connil, Nathalie
Orange, Nicole
Feuilloley, Marc GJ
Merieau, Annabelle
author_facet Sperandio, Daniel
Rossignol, Gaelle
Guerillon, Josette
Connil, Nathalie
Orange, Nicole
Feuilloley, Marc GJ
Merieau, Annabelle
author_sort Sperandio, Daniel
collection PubMed
description BACKGROUND: MFN1032 is a clinical Pseudomonas fluorescens strain able to grow at 37°C. MFN1032 cells induce necrosis and apoptosis in rat glial cells at this temperature. This strain displays secretion-mediated hemolytic activity involving phospholipase C and cyclolipopeptides. Under laboratory conditions, this activity is not expressed at 37°C. This activity is tightly regulated and is subject to phase variation. RESULTS: We found that MFN1032 displays a cell-associated hemolytic activity distinct from the secreted hemolytic activity. Cell-associated hemolysis was expressed at 37°C and was only detected in vitro in mid log growth phase in the presence of erythrocytes. We studied the regulation of this activity in the wild-type strain and in a mutant defective in the Gac two-component pathway. GacS/GacA is a negative regulator of this activity. In contrast to the Pseudomonas fluorescens strains PfO-1 and Pf5, whose genomes have been sequenced, the MFN1032 strain has the type III secretion-like genes hrcRST belonging to the hrpU operon. We showed that disruption of this operon abolished cell-associated hemolytic activity. This activity was not detected in P.fluorescens strains carrying similar hrc genes, as for the P. fluorescens psychrotrophic strain MF37. CONCLUSIONS: To our knowledge this the first demonstration of cell-associated hemolytic activity of a clinical strain of Pseudomonas fluorescens. Moreover, this activity seems to be related to a functional hrpU operon and is independent of biosurfactant production. Precise link between a functional hrpU operon and cell-associated hemolytic activity remains to be elucidated.
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spelling pubmed-28712722010-05-17 Cell-associated hemolysis activity in the clinical strain of Pseudomonas fluorescens MFN1032 Sperandio, Daniel Rossignol, Gaelle Guerillon, Josette Connil, Nathalie Orange, Nicole Feuilloley, Marc GJ Merieau, Annabelle BMC Microbiol Research article BACKGROUND: MFN1032 is a clinical Pseudomonas fluorescens strain able to grow at 37°C. MFN1032 cells induce necrosis and apoptosis in rat glial cells at this temperature. This strain displays secretion-mediated hemolytic activity involving phospholipase C and cyclolipopeptides. Under laboratory conditions, this activity is not expressed at 37°C. This activity is tightly regulated and is subject to phase variation. RESULTS: We found that MFN1032 displays a cell-associated hemolytic activity distinct from the secreted hemolytic activity. Cell-associated hemolysis was expressed at 37°C and was only detected in vitro in mid log growth phase in the presence of erythrocytes. We studied the regulation of this activity in the wild-type strain and in a mutant defective in the Gac two-component pathway. GacS/GacA is a negative regulator of this activity. In contrast to the Pseudomonas fluorescens strains PfO-1 and Pf5, whose genomes have been sequenced, the MFN1032 strain has the type III secretion-like genes hrcRST belonging to the hrpU operon. We showed that disruption of this operon abolished cell-associated hemolytic activity. This activity was not detected in P.fluorescens strains carrying similar hrc genes, as for the P. fluorescens psychrotrophic strain MF37. CONCLUSIONS: To our knowledge this the first demonstration of cell-associated hemolytic activity of a clinical strain of Pseudomonas fluorescens. Moreover, this activity seems to be related to a functional hrpU operon and is independent of biosurfactant production. Precise link between a functional hrpU operon and cell-associated hemolytic activity remains to be elucidated. BioMed Central 2010-04-24 /pmc/articles/PMC2871272/ /pubmed/20416103 http://dx.doi.org/10.1186/1471-2180-10-124 Text en Copyright ©2010 Sperandio et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research article
Sperandio, Daniel
Rossignol, Gaelle
Guerillon, Josette
Connil, Nathalie
Orange, Nicole
Feuilloley, Marc GJ
Merieau, Annabelle
Cell-associated hemolysis activity in the clinical strain of Pseudomonas fluorescens MFN1032
title Cell-associated hemolysis activity in the clinical strain of Pseudomonas fluorescens MFN1032
title_full Cell-associated hemolysis activity in the clinical strain of Pseudomonas fluorescens MFN1032
title_fullStr Cell-associated hemolysis activity in the clinical strain of Pseudomonas fluorescens MFN1032
title_full_unstemmed Cell-associated hemolysis activity in the clinical strain of Pseudomonas fluorescens MFN1032
title_short Cell-associated hemolysis activity in the clinical strain of Pseudomonas fluorescens MFN1032
title_sort cell-associated hemolysis activity in the clinical strain of pseudomonas fluorescens mfn1032
topic Research article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2871272/
https://www.ncbi.nlm.nih.gov/pubmed/20416103
http://dx.doi.org/10.1186/1471-2180-10-124
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