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Modification of maturation condition improves oocyte maturation and in vitro development of somatic cell nuclear transfer pig embryos
This study examined effects on the developmental competence of pig oocytes after somatic cell nuclear transfer (SCNT) or parthenogenetic activation (PA) of : 1) co-culturing of oocytes with follicular shell pieces (FSP) during in vitro maturation (IVM); 2) different durations of maturation; and 3) d...
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Formato: | Texto |
Lenguaje: | English |
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The Korean Society of Veterinary Science
2007
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2872702/ https://www.ncbi.nlm.nih.gov/pubmed/17322778 http://dx.doi.org/10.4142/jvs.2007.8.1.81 |
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author | Song, Kilyoung Lee, Eunsong |
author_facet | Song, Kilyoung Lee, Eunsong |
author_sort | Song, Kilyoung |
collection | PubMed |
description | This study examined effects on the developmental competence of pig oocytes after somatic cell nuclear transfer (SCNT) or parthenogenetic activation (PA) of : 1) co-culturing of oocytes with follicular shell pieces (FSP) during in vitro maturation (IVM); 2) different durations of maturation; and 3) defined maturation medium supplemented with polyvinyl alcohol (PVA; control), pig follicular fluid (pFF), cysteamine (CYS), or β-mercaptoethanol (β-ME). The proportion of metaphase II oocytes was increased (p < 0.05) by co-culturing with FSP compared to control oocytes (98% vs. 94%). However, blastocyst formation after SCNT was not improved by FSP coculture (9% vs. 12%). Nuclear maturation of oocytes matured for 39 or 42 h was higher (p < 0.05) than that of oocytes matured for 36 h (95-96% vs. 79%). Cleavage (83%) and blastocyst formation (26%) were significantly higher (p < 0.05) in oocytes matured for 42 h than in other groups. Supplementation of a defined maturation medium with 100 µM CYS or 100 µM β-ME showed no stimulatory effect on oocyte maturation, embryo cleavage, or blastocyst formation after PA. β-ME treatment during IVM decreased embryo cleavage after SCNT compared to pFF or PVA treatments, but no significant difference was found in blastocyst formation (7-16%) among the four treatment groups. The results indicated that maturation of oocytes for 42 h was beneficial for the development of SCNT embryos. Furthermore, the defined maturation system used in this study could support in vitro development of PA or SCNT embryos. |
format | Text |
id | pubmed-2872702 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | The Korean Society of Veterinary Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-28727022010-05-19 Modification of maturation condition improves oocyte maturation and in vitro development of somatic cell nuclear transfer pig embryos Song, Kilyoung Lee, Eunsong J Vet Sci Original Article This study examined effects on the developmental competence of pig oocytes after somatic cell nuclear transfer (SCNT) or parthenogenetic activation (PA) of : 1) co-culturing of oocytes with follicular shell pieces (FSP) during in vitro maturation (IVM); 2) different durations of maturation; and 3) defined maturation medium supplemented with polyvinyl alcohol (PVA; control), pig follicular fluid (pFF), cysteamine (CYS), or β-mercaptoethanol (β-ME). The proportion of metaphase II oocytes was increased (p < 0.05) by co-culturing with FSP compared to control oocytes (98% vs. 94%). However, blastocyst formation after SCNT was not improved by FSP coculture (9% vs. 12%). Nuclear maturation of oocytes matured for 39 or 42 h was higher (p < 0.05) than that of oocytes matured for 36 h (95-96% vs. 79%). Cleavage (83%) and blastocyst formation (26%) were significantly higher (p < 0.05) in oocytes matured for 42 h than in other groups. Supplementation of a defined maturation medium with 100 µM CYS or 100 µM β-ME showed no stimulatory effect on oocyte maturation, embryo cleavage, or blastocyst formation after PA. β-ME treatment during IVM decreased embryo cleavage after SCNT compared to pFF or PVA treatments, but no significant difference was found in blastocyst formation (7-16%) among the four treatment groups. The results indicated that maturation of oocytes for 42 h was beneficial for the development of SCNT embryos. Furthermore, the defined maturation system used in this study could support in vitro development of PA or SCNT embryos. The Korean Society of Veterinary Science 2007-03 2007-03-31 /pmc/articles/PMC2872702/ /pubmed/17322778 http://dx.doi.org/10.4142/jvs.2007.8.1.81 Text en Copyright © 2007 The Korean Society of Veterinary Science https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Song, Kilyoung Lee, Eunsong Modification of maturation condition improves oocyte maturation and in vitro development of somatic cell nuclear transfer pig embryos |
title | Modification of maturation condition improves oocyte maturation and in vitro development of somatic cell nuclear transfer pig embryos |
title_full | Modification of maturation condition improves oocyte maturation and in vitro development of somatic cell nuclear transfer pig embryos |
title_fullStr | Modification of maturation condition improves oocyte maturation and in vitro development of somatic cell nuclear transfer pig embryos |
title_full_unstemmed | Modification of maturation condition improves oocyte maturation and in vitro development of somatic cell nuclear transfer pig embryos |
title_short | Modification of maturation condition improves oocyte maturation and in vitro development of somatic cell nuclear transfer pig embryos |
title_sort | modification of maturation condition improves oocyte maturation and in vitro development of somatic cell nuclear transfer pig embryos |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2872702/ https://www.ncbi.nlm.nih.gov/pubmed/17322778 http://dx.doi.org/10.4142/jvs.2007.8.1.81 |
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