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PCR-based detection of genes encoding virulence determinants in Staphylococcus aureus from bovine subclinical mastitis cases
The present study was carried out to genotypically characterize Staphylococcus aureus (S. aureus) isolated from bovine mastitis cases. A total of 37 strains of S. aureus were isolated during processing of 552 milk samples from 140 cows. The S. aureus strains were characterized phenotypically, and we...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
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The Korean Society of Veterinary Science
2007
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2872713/ https://www.ncbi.nlm.nih.gov/pubmed/17519568 http://dx.doi.org/10.4142/jvs.2007.8.2.151 |
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author | Kalorey, Dewanand Rajaram Shanmugam, Yuvaraj Kurkure, Nitin Vasantrao Chousalkar, Kapil Kamalakarrao Barbuddhe, Sukhadeo Baliram |
author_facet | Kalorey, Dewanand Rajaram Shanmugam, Yuvaraj Kurkure, Nitin Vasantrao Chousalkar, Kapil Kamalakarrao Barbuddhe, Sukhadeo Baliram |
author_sort | Kalorey, Dewanand Rajaram |
collection | PubMed |
description | The present study was carried out to genotypically characterize Staphylococcus aureus (S. aureus) isolated from bovine mastitis cases. A total of 37 strains of S. aureus were isolated during processing of 552 milk samples from 140 cows. The S. aureus strains were characterized phenotypically, and were further characterized genotypically by polymerase chain reaction using oligonucleotide primers that amplified genes encoding coagulase (coa), clumping factor (clfA), thermonuclease (nuc), enterotoxin A (entA), and the gene segments encoding the immunoglobulin G binding region and the X region of protein A gene spa. All of the isolates yielded an amplicon with a size of approximately 1,042 bp of the clfA gene. The amplification of the polymorphic spa gene segment encoding the immunoglobulin G binding region was observed in 34 isolates and X-region binding was detected in 26 isolates. Amplification of the coa gene yielded three different products in 20, 10, and 7 isolates. The amplification of the thermonuclease gene, nuc, was observed in 36 out of 37 isolates. All of the samples were negative for the entA gene. The phenotypic and genotypic findings of the present strategies might provide an understanding of the distribution of the prevalent S. aureus clones among bovine mastitis isolates, and might aid in the development of steps to control S. aureus infections in dairy herds. |
format | Text |
id | pubmed-2872713 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | The Korean Society of Veterinary Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-28727132010-05-19 PCR-based detection of genes encoding virulence determinants in Staphylococcus aureus from bovine subclinical mastitis cases Kalorey, Dewanand Rajaram Shanmugam, Yuvaraj Kurkure, Nitin Vasantrao Chousalkar, Kapil Kamalakarrao Barbuddhe, Sukhadeo Baliram J Vet Sci Original Article The present study was carried out to genotypically characterize Staphylococcus aureus (S. aureus) isolated from bovine mastitis cases. A total of 37 strains of S. aureus were isolated during processing of 552 milk samples from 140 cows. The S. aureus strains were characterized phenotypically, and were further characterized genotypically by polymerase chain reaction using oligonucleotide primers that amplified genes encoding coagulase (coa), clumping factor (clfA), thermonuclease (nuc), enterotoxin A (entA), and the gene segments encoding the immunoglobulin G binding region and the X region of protein A gene spa. All of the isolates yielded an amplicon with a size of approximately 1,042 bp of the clfA gene. The amplification of the polymorphic spa gene segment encoding the immunoglobulin G binding region was observed in 34 isolates and X-region binding was detected in 26 isolates. Amplification of the coa gene yielded three different products in 20, 10, and 7 isolates. The amplification of the thermonuclease gene, nuc, was observed in 36 out of 37 isolates. All of the samples were negative for the entA gene. The phenotypic and genotypic findings of the present strategies might provide an understanding of the distribution of the prevalent S. aureus clones among bovine mastitis isolates, and might aid in the development of steps to control S. aureus infections in dairy herds. The Korean Society of Veterinary Science 2007-06 2007-06-30 /pmc/articles/PMC2872713/ /pubmed/17519568 http://dx.doi.org/10.4142/jvs.2007.8.2.151 Text en Copyright © 2007 The Korean Society of Veterinary Science https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Kalorey, Dewanand Rajaram Shanmugam, Yuvaraj Kurkure, Nitin Vasantrao Chousalkar, Kapil Kamalakarrao Barbuddhe, Sukhadeo Baliram PCR-based detection of genes encoding virulence determinants in Staphylococcus aureus from bovine subclinical mastitis cases |
title | PCR-based detection of genes encoding virulence determinants in Staphylococcus aureus from bovine subclinical mastitis cases |
title_full | PCR-based detection of genes encoding virulence determinants in Staphylococcus aureus from bovine subclinical mastitis cases |
title_fullStr | PCR-based detection of genes encoding virulence determinants in Staphylococcus aureus from bovine subclinical mastitis cases |
title_full_unstemmed | PCR-based detection of genes encoding virulence determinants in Staphylococcus aureus from bovine subclinical mastitis cases |
title_short | PCR-based detection of genes encoding virulence determinants in Staphylococcus aureus from bovine subclinical mastitis cases |
title_sort | pcr-based detection of genes encoding virulence determinants in staphylococcus aureus from bovine subclinical mastitis cases |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2872713/ https://www.ncbi.nlm.nih.gov/pubmed/17519568 http://dx.doi.org/10.4142/jvs.2007.8.2.151 |
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