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A scalable, fully automated process for construction of sequence-ready barcoded libraries for 454
We present an automated, high throughput library construction process for 454 technology. Sample handling errors and cross-contamination are minimized via end-to-end barcoding of plasticware, along with molecular DNA barcoding of constructs. Automation-friendly magnetic bead-based size selection and...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2010
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2872875/ https://www.ncbi.nlm.nih.gov/pubmed/20137071 http://dx.doi.org/10.1186/gb-2010-11-2-r15 |
| _version_ | 1782181278005592064 |
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| author | Lennon, Niall J Lintner, Robert E Anderson, Scott Alvarez, Pablo Barry, Andrew Brockman, William Daza, Riza Erlich, Rachel L Giannoukos, Georgia Green, Lisa Hollinger, Andrew Hoover, Cindi A Jaffe, David B Juhn, Frank McCarthy, Danielle Perrin, Danielle Ponchner, Karen Powers, Taryn L Rizzolo, Kamran Robbins, Dana Ryan, Elizabeth Russ, Carsten Sparrow, Todd Stalker, John Steelman, Scott Weiand, Michael Zimmer, Andrew Henn, Matthew R Nusbaum, Chad Nicol, Robert |
| author_facet | Lennon, Niall J Lintner, Robert E Anderson, Scott Alvarez, Pablo Barry, Andrew Brockman, William Daza, Riza Erlich, Rachel L Giannoukos, Georgia Green, Lisa Hollinger, Andrew Hoover, Cindi A Jaffe, David B Juhn, Frank McCarthy, Danielle Perrin, Danielle Ponchner, Karen Powers, Taryn L Rizzolo, Kamran Robbins, Dana Ryan, Elizabeth Russ, Carsten Sparrow, Todd Stalker, John Steelman, Scott Weiand, Michael Zimmer, Andrew Henn, Matthew R Nusbaum, Chad Nicol, Robert |
| author_sort | Lennon, Niall J |
| collection | PubMed |
| description | We present an automated, high throughput library construction process for 454 technology. Sample handling errors and cross-contamination are minimized via end-to-end barcoding of plasticware, along with molecular DNA barcoding of constructs. Automation-friendly magnetic bead-based size selection and cleanup steps have been devised, eliminating major bottlenecks and significant sources of error. Using this methodology, one technician can create 96 sequence-ready 454 libraries in 2 days, a dramatic improvement over the standard method. |
| format | Text |
| id | pubmed-2872875 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2010 |
| publisher | BioMed Central |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-28728752010-05-20 A scalable, fully automated process for construction of sequence-ready barcoded libraries for 454 Lennon, Niall J Lintner, Robert E Anderson, Scott Alvarez, Pablo Barry, Andrew Brockman, William Daza, Riza Erlich, Rachel L Giannoukos, Georgia Green, Lisa Hollinger, Andrew Hoover, Cindi A Jaffe, David B Juhn, Frank McCarthy, Danielle Perrin, Danielle Ponchner, Karen Powers, Taryn L Rizzolo, Kamran Robbins, Dana Ryan, Elizabeth Russ, Carsten Sparrow, Todd Stalker, John Steelman, Scott Weiand, Michael Zimmer, Andrew Henn, Matthew R Nusbaum, Chad Nicol, Robert Genome Biol Method We present an automated, high throughput library construction process for 454 technology. Sample handling errors and cross-contamination are minimized via end-to-end barcoding of plasticware, along with molecular DNA barcoding of constructs. Automation-friendly magnetic bead-based size selection and cleanup steps have been devised, eliminating major bottlenecks and significant sources of error. Using this methodology, one technician can create 96 sequence-ready 454 libraries in 2 days, a dramatic improvement over the standard method. BioMed Central 2010 2010-02-05 /pmc/articles/PMC2872875/ /pubmed/20137071 http://dx.doi.org/10.1186/gb-2010-11-2-r15 Text en Copyright ©2010 Lennon et al.; license BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| spellingShingle | Method Lennon, Niall J Lintner, Robert E Anderson, Scott Alvarez, Pablo Barry, Andrew Brockman, William Daza, Riza Erlich, Rachel L Giannoukos, Georgia Green, Lisa Hollinger, Andrew Hoover, Cindi A Jaffe, David B Juhn, Frank McCarthy, Danielle Perrin, Danielle Ponchner, Karen Powers, Taryn L Rizzolo, Kamran Robbins, Dana Ryan, Elizabeth Russ, Carsten Sparrow, Todd Stalker, John Steelman, Scott Weiand, Michael Zimmer, Andrew Henn, Matthew R Nusbaum, Chad Nicol, Robert A scalable, fully automated process for construction of sequence-ready barcoded libraries for 454 |
| title | A scalable, fully automated process for construction of sequence-ready barcoded libraries for 454 |
| title_full | A scalable, fully automated process for construction of sequence-ready barcoded libraries for 454 |
| title_fullStr | A scalable, fully automated process for construction of sequence-ready barcoded libraries for 454 |
| title_full_unstemmed | A scalable, fully automated process for construction of sequence-ready barcoded libraries for 454 |
| title_short | A scalable, fully automated process for construction of sequence-ready barcoded libraries for 454 |
| title_sort | scalable, fully automated process for construction of sequence-ready barcoded libraries for 454 |
| topic | Method |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2872875/ https://www.ncbi.nlm.nih.gov/pubmed/20137071 http://dx.doi.org/10.1186/gb-2010-11-2-r15 |
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