In Vitro Nuclear Assembly Using Fractionated Xenopus Egg Extracts

Nuclear membrane assembly is an essential step in the cell division cycle; this process can be replicated in the test tube by combining Xenopus sperm chromatin, cytosol, and light membrane fractions. Complete nuclei are formed, including nuclear membranes with pore complexes, and these reconstituted...

Descripción completa

Detalles Bibliográficos
Autores principales: Cross, Marie, Powers, Maureen
Formato: Texto
Lenguaje:English
Publicado: MyJove Corporation 2008
Materias:
Cellular Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2872975/
https://www.ncbi.nlm.nih.gov/pubmed/19066496
http://dx.doi.org/10.3791/908
_version_ 1782181289487499264
author Cross, Marie
Powers, Maureen
author_facet Cross, Marie
Powers, Maureen
author_sort Cross, Marie
collection PubMed
description Nuclear membrane assembly is an essential step in the cell division cycle; this process can be replicated in the test tube by combining Xenopus sperm chromatin, cytosol, and light membrane fractions. Complete nuclei are formed, including nuclear membranes with pore complexes, and these reconstituted nuclei are capable of normal nuclear processes.
format Text
id pubmed-2872975
institution National Center for Biotechnology Information
language English
publishDate 2008
publisher MyJove Corporation
record_format MEDLINE/PubMed
spelling pubmed-28729752011-03-15 In Vitro Nuclear Assembly Using Fractionated Xenopus Egg Extracts Cross, Marie Powers, Maureen J Vis Exp Cellular Biology Nuclear membrane assembly is an essential step in the cell division cycle; this process can be replicated in the test tube by combining Xenopus sperm chromatin, cytosol, and light membrane fractions. Complete nuclei are formed, including nuclear membranes with pore complexes, and these reconstituted nuclei are capable of normal nuclear processes. MyJove Corporation 2008-09-02 /pmc/articles/PMC2872975/ /pubmed/19066496 http://dx.doi.org/10.3791/908 Text en Copyright © 2008, Journal of Visualized Experiments http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Cellular Biology
Cross, Marie
Powers, Maureen
In Vitro Nuclear Assembly Using Fractionated Xenopus Egg Extracts
title In Vitro Nuclear Assembly Using Fractionated Xenopus Egg Extracts
title_full In Vitro Nuclear Assembly Using Fractionated Xenopus Egg Extracts
title_fullStr In Vitro Nuclear Assembly Using Fractionated Xenopus Egg Extracts
title_full_unstemmed In Vitro Nuclear Assembly Using Fractionated Xenopus Egg Extracts
title_short In Vitro Nuclear Assembly Using Fractionated Xenopus Egg Extracts
title_sort in vitro nuclear assembly using fractionated xenopus egg extracts
topic Cellular Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2872975/
https://www.ncbi.nlm.nih.gov/pubmed/19066496
http://dx.doi.org/10.3791/908
work_keys_str_mv AT crossmarie invitronuclearassemblyusingfractionatedxenopuseggextracts
AT powersmaureen invitronuclearassemblyusingfractionatedxenopuseggextracts

Ejemplares similares