Reprogramming Bacteria to Seek and Destroy a Herbicide

A major goal of synthetic biology is to reprogram cells to perform complex tasks. Here we show how a combination of in vitro and in vivo selection rapidly identifies a synthetic riboswitch that activates protein translation in response to the herbicide atrazine. We further demonstrate that this ribo...

Descripción completa

Detalles Bibliográficos
Autores principales: Sinha, Joy, Reyes, Samuel J., Gallivan, Justin P.
Formato: Texto
Lenguaje:English
Publicado: 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2873063/
https://www.ncbi.nlm.nih.gov/pubmed/20453864
http://dx.doi.org/10.1038/nchembio.369
_version_ 1782181291853086720
author Sinha, Joy
Reyes, Samuel J.
Gallivan, Justin P.
author_facet Sinha, Joy
Reyes, Samuel J.
Gallivan, Justin P.
author_sort Sinha, Joy
collection PubMed
description A major goal of synthetic biology is to reprogram cells to perform complex tasks. Here we show how a combination of in vitro and in vivo selection rapidly identifies a synthetic riboswitch that activates protein translation in response to the herbicide atrazine. We further demonstrate that this riboswitch can reprogram bacteria to migrate in the presence of atrazine. Finally, we show that incorporating a gene from an atrazine catabolic pathway allows these cells to seek and destroy atrazine.
format Text
id pubmed-2873063
institution National Center for Biotechnology Information
language English
publishDate 2010
record_format MEDLINE/PubMed
spelling pubmed-28730632010-12-01 Reprogramming Bacteria to Seek and Destroy a Herbicide Sinha, Joy Reyes, Samuel J. Gallivan, Justin P. Nat Chem Biol Article A major goal of synthetic biology is to reprogram cells to perform complex tasks. Here we show how a combination of in vitro and in vivo selection rapidly identifies a synthetic riboswitch that activates protein translation in response to the herbicide atrazine. We further demonstrate that this riboswitch can reprogram bacteria to migrate in the presence of atrazine. Finally, we show that incorporating a gene from an atrazine catabolic pathway allows these cells to seek and destroy atrazine. 2010-05-09 2010-06 /pmc/articles/PMC2873063/ /pubmed/20453864 http://dx.doi.org/10.1038/nchembio.369 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Sinha, Joy
Reyes, Samuel J.
Gallivan, Justin P.
Reprogramming Bacteria to Seek and Destroy a Herbicide
title Reprogramming Bacteria to Seek and Destroy a Herbicide
title_full Reprogramming Bacteria to Seek and Destroy a Herbicide
title_fullStr Reprogramming Bacteria to Seek and Destroy a Herbicide
title_full_unstemmed Reprogramming Bacteria to Seek and Destroy a Herbicide
title_short Reprogramming Bacteria to Seek and Destroy a Herbicide
title_sort reprogramming bacteria to seek and destroy a herbicide
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2873063/
https://www.ncbi.nlm.nih.gov/pubmed/20453864
http://dx.doi.org/10.1038/nchembio.369
work_keys_str_mv AT sinhajoy reprogrammingbacteriatoseekanddestroyaherbicide
AT reyessamuelj reprogrammingbacteriatoseekanddestroyaherbicide
AT gallivanjustinp reprogrammingbacteriatoseekanddestroyaherbicide

Ejemplares similares