Live cell flattening — traditional and novel approaches

Eukaryotic cell flattening is valuable for improving microscopic observations, ranging from bright field (BF) to total internal reflection fluorescence (TIRF) microscopy. Fundamental processes, such as mitosis and in vivo actin polymerization, have been investigated using these techniques. Here, we...

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Detalles Bibliográficos
Autores principales: Westendorf, Christian, Bae, Albert J, Erlenkamper, Christoph, Galland, Edouard, Franck, Carl, Bodenschatz, Eberhard, Beta, Carsten
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Research article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2873278/
https://www.ncbi.nlm.nih.gov/pubmed/20403171
http://dx.doi.org/10.1186/1757-5036-3-9
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author Westendorf, Christian
Bae, Albert J
Erlenkamper, Christoph
Galland, Edouard
Franck, Carl
Bodenschatz, Eberhard
Beta, Carsten
author_facet Westendorf, Christian
Bae, Albert J
Erlenkamper, Christoph
Galland, Edouard
Franck, Carl
Bodenschatz, Eberhard
Beta, Carsten
author_sort Westendorf, Christian
collection PubMed
description Eukaryotic cell flattening is valuable for improving microscopic observations, ranging from bright field (BF) to total internal reflection fluorescence (TIRF) microscopy. Fundamental processes, such as mitosis and in vivo actin polymerization, have been investigated using these techniques. Here, we review the well known agar overlayer protocol and the oil overlay method. In addition, we present more elaborate microfluidics-based techniques that provide us with a greater level of control. We demonstrate these techniques on the social amoebae Dictyostelium discoideum, comparing the advantages and disadvantages of each method. PACS Codes: 87.64.-t, 47.61.-k, 87.80.Ek
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spelling pubmed-28732782010-05-20 Live cell flattening — traditional and novel approaches Westendorf, Christian Bae, Albert J Erlenkamper, Christoph Galland, Edouard Franck, Carl Bodenschatz, Eberhard Beta, Carsten PMC Biophys Research article Eukaryotic cell flattening is valuable for improving microscopic observations, ranging from bright field (BF) to total internal reflection fluorescence (TIRF) microscopy. Fundamental processes, such as mitosis and in vivo actin polymerization, have been investigated using these techniques. Here, we review the well known agar overlayer protocol and the oil overlay method. In addition, we present more elaborate microfluidics-based techniques that provide us with a greater level of control. We demonstrate these techniques on the social amoebae Dictyostelium discoideum, comparing the advantages and disadvantages of each method. PACS Codes: 87.64.-t, 47.61.-k, 87.80.Ek BioMed Central 2010-04-19 /pmc/articles/PMC2873278/ /pubmed/20403171 http://dx.doi.org/10.1186/1757-5036-3-9 Text en Copyright ©2010 Beta et al http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research article
Westendorf, Christian
Bae, Albert J
Erlenkamper, Christoph
Galland, Edouard
Franck, Carl
Bodenschatz, Eberhard
Beta, Carsten
Live cell flattening — traditional and novel approaches
title Live cell flattening — traditional and novel approaches
title_full Live cell flattening — traditional and novel approaches
title_fullStr Live cell flattening — traditional and novel approaches
title_full_unstemmed Live cell flattening — traditional and novel approaches
title_short Live cell flattening — traditional and novel approaches
title_sort live cell flattening — traditional and novel approaches
topic Research article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2873278/
https://www.ncbi.nlm.nih.gov/pubmed/20403171
http://dx.doi.org/10.1186/1757-5036-3-9
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AT gallandedouard livecellflatteningtraditionalandnovelapproaches
AT franckcarl livecellflatteningtraditionalandnovelapproaches
AT bodenschatzeberhard livecellflatteningtraditionalandnovelapproaches
AT betacarsten livecellflatteningtraditionalandnovelapproaches

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