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Detection of tmRNA molecules on microarrays at low temperatures using helper oligonucleotides
BACKGROUND: The hybridization of synthetic Streptococcus pneumoniae tmRNA on a detection microarray is slow at 34°C resulting in low signal intensities. RESULTS: We demonstrate that adding specific DNA helper oligonucleotides (chaperones) to the hybridization buffer increases the signal strength at...
| Autores principales: | , , , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2010
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2873282/ https://www.ncbi.nlm.nih.gov/pubmed/20426847 http://dx.doi.org/10.1186/1472-6750-10-34 |