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Fowlpox virus recombinants expressing HPV-16 E6 and E7 oncogenes for the therapy of cervical carcinoma elicit humoral and cell-mediated responses in rabbits
BACKGROUND: Around half million new cases of cervical cancer arise each year, making the development of an effective therapeutic vaccine against HPV a high priority. As the E6 and E7 oncoproteins are expressed in all HPV-16 tumour cells, vaccines expressing these proteins might clear an already esta...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2010
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2873375/ https://www.ncbi.nlm.nih.gov/pubmed/20409340 http://dx.doi.org/10.1186/1479-5876-8-40 |
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author | Radaelli, Antonia Pozzi, Eleana Pacchioni, Sole Zanotto, Carlo Morghen, Carlo De Giuli |
author_facet | Radaelli, Antonia Pozzi, Eleana Pacchioni, Sole Zanotto, Carlo Morghen, Carlo De Giuli |
author_sort | Radaelli, Antonia |
collection | PubMed |
description | BACKGROUND: Around half million new cases of cervical cancer arise each year, making the development of an effective therapeutic vaccine against HPV a high priority. As the E6 and E7 oncoproteins are expressed in all HPV-16 tumour cells, vaccines expressing these proteins might clear an already established tumour and support the treatment of HPV-related precancerous lesions. METHODS: Three different immunisation regimens were tested in a pre-clinical trial in rabbits to evaluate the humoral and cell-mediated responses of a putative HPV-16 vaccine. Fowlpoxvirus (FP) recombinants separately expressing the HPV-16 E6 (FP(E6)) and E7 (FP(E7)) transgenes were used for priming, followed by E7 protein boosting. RESULTS: All of the protocols were effective in eliciting a high antibody response. This was also confirmed by interleukin-4 production, which increased after simultaneous priming with both FP(E6 )and FP(E7 )and after E7 protein boost. A cell-mediated immune response was also detected in most of the animals. CONCLUSION: These results establish a preliminary profile for the therapy with the combined use of avipox recombinants, which may represent safer immunogens than vaccinia-based vectors in immuno-compromised individuals, as they express the transgenes in most mammalian cells in the absence of a productive replication. |
format | Text |
id | pubmed-2873375 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-28733752010-05-20 Fowlpox virus recombinants expressing HPV-16 E6 and E7 oncogenes for the therapy of cervical carcinoma elicit humoral and cell-mediated responses in rabbits Radaelli, Antonia Pozzi, Eleana Pacchioni, Sole Zanotto, Carlo Morghen, Carlo De Giuli J Transl Med Research BACKGROUND: Around half million new cases of cervical cancer arise each year, making the development of an effective therapeutic vaccine against HPV a high priority. As the E6 and E7 oncoproteins are expressed in all HPV-16 tumour cells, vaccines expressing these proteins might clear an already established tumour and support the treatment of HPV-related precancerous lesions. METHODS: Three different immunisation regimens were tested in a pre-clinical trial in rabbits to evaluate the humoral and cell-mediated responses of a putative HPV-16 vaccine. Fowlpoxvirus (FP) recombinants separately expressing the HPV-16 E6 (FP(E6)) and E7 (FP(E7)) transgenes were used for priming, followed by E7 protein boosting. RESULTS: All of the protocols were effective in eliciting a high antibody response. This was also confirmed by interleukin-4 production, which increased after simultaneous priming with both FP(E6 )and FP(E7 )and after E7 protein boost. A cell-mediated immune response was also detected in most of the animals. CONCLUSION: These results establish a preliminary profile for the therapy with the combined use of avipox recombinants, which may represent safer immunogens than vaccinia-based vectors in immuno-compromised individuals, as they express the transgenes in most mammalian cells in the absence of a productive replication. BioMed Central 2010-04-21 /pmc/articles/PMC2873375/ /pubmed/20409340 http://dx.doi.org/10.1186/1479-5876-8-40 Text en Copyright ©2010 Radaelli et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Radaelli, Antonia Pozzi, Eleana Pacchioni, Sole Zanotto, Carlo Morghen, Carlo De Giuli Fowlpox virus recombinants expressing HPV-16 E6 and E7 oncogenes for the therapy of cervical carcinoma elicit humoral and cell-mediated responses in rabbits |
title | Fowlpox virus recombinants expressing HPV-16 E6 and E7 oncogenes for the therapy of cervical carcinoma elicit humoral and cell-mediated responses in rabbits |
title_full | Fowlpox virus recombinants expressing HPV-16 E6 and E7 oncogenes for the therapy of cervical carcinoma elicit humoral and cell-mediated responses in rabbits |
title_fullStr | Fowlpox virus recombinants expressing HPV-16 E6 and E7 oncogenes for the therapy of cervical carcinoma elicit humoral and cell-mediated responses in rabbits |
title_full_unstemmed | Fowlpox virus recombinants expressing HPV-16 E6 and E7 oncogenes for the therapy of cervical carcinoma elicit humoral and cell-mediated responses in rabbits |
title_short | Fowlpox virus recombinants expressing HPV-16 E6 and E7 oncogenes for the therapy of cervical carcinoma elicit humoral and cell-mediated responses in rabbits |
title_sort | fowlpox virus recombinants expressing hpv-16 e6 and e7 oncogenes for the therapy of cervical carcinoma elicit humoral and cell-mediated responses in rabbits |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2873375/ https://www.ncbi.nlm.nih.gov/pubmed/20409340 http://dx.doi.org/10.1186/1479-5876-8-40 |
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