Cargando…

Age-related molecular genetic changes of murine bone marrow mesenchymal stem cells

BACKGROUND: Mesenchymal stem cells (MSC) are pluripotent cells, present in the bone marrow and other tissues that can differentiate into cells of all germ layers and may be involved in tissue maintenance and repair in adult organisms. Because of their plasticity and accessibility these cells are als...

Descripción completa

Detalles Bibliográficos
Autores principales: Wilson, Amber, Shehadeh, Lina A, Yu, Hong, Webster, Keith A
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2873471/
https://www.ncbi.nlm.nih.gov/pubmed/20374652
http://dx.doi.org/10.1186/1471-2164-11-229
_version_ 1782181348372381696
author Wilson, Amber
Shehadeh, Lina A
Yu, Hong
Webster, Keith A
author_facet Wilson, Amber
Shehadeh, Lina A
Yu, Hong
Webster, Keith A
author_sort Wilson, Amber
collection PubMed
description BACKGROUND: Mesenchymal stem cells (MSC) are pluripotent cells, present in the bone marrow and other tissues that can differentiate into cells of all germ layers and may be involved in tissue maintenance and repair in adult organisms. Because of their plasticity and accessibility these cells are also prime candidates for regenerative medicine. The contribution of stem cell aging to organismal aging is under debate and one theory is that reparative processes deteriorate as a consequence of stem cell aging and/or decrease in number. Age has been linked with changes in osteogenic and adipogenic potential of MSCs. RESULTS: Here we report on changes in global gene expression of cultured MSCs isolated from the bone marrow of mice at ages 2, 8, and 26-months. Microarray analyses revealed significant changes in the expression of more than 8000 genes with stage-specific changes of multiple differentiation, cell cycle and growth factor genes. Key markers of adipogenesis including lipoprotein lipase, FABP4, and Itm2a displayed age-dependent declines. Expression of the master cell cycle regulators p53 and p21 and growth factors HGF and VEGF also declined significantly at 26 months. These changes were evident despite multiple cell divisions in vitro after bone marrow isolation. CONCLUSIONS: The results suggest that MSCs are subject to molecular genetic changes during aging that are conserved during passage in culture. These changes may affect the physiological functions and the potential of autologous MSCs for stem cell therapy.
format Text
id pubmed-2873471
institution National Center for Biotechnology Information
language English
publishDate 2010
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-28734712010-05-20 Age-related molecular genetic changes of murine bone marrow mesenchymal stem cells Wilson, Amber Shehadeh, Lina A Yu, Hong Webster, Keith A BMC Genomics Research Article BACKGROUND: Mesenchymal stem cells (MSC) are pluripotent cells, present in the bone marrow and other tissues that can differentiate into cells of all germ layers and may be involved in tissue maintenance and repair in adult organisms. Because of their plasticity and accessibility these cells are also prime candidates for regenerative medicine. The contribution of stem cell aging to organismal aging is under debate and one theory is that reparative processes deteriorate as a consequence of stem cell aging and/or decrease in number. Age has been linked with changes in osteogenic and adipogenic potential of MSCs. RESULTS: Here we report on changes in global gene expression of cultured MSCs isolated from the bone marrow of mice at ages 2, 8, and 26-months. Microarray analyses revealed significant changes in the expression of more than 8000 genes with stage-specific changes of multiple differentiation, cell cycle and growth factor genes. Key markers of adipogenesis including lipoprotein lipase, FABP4, and Itm2a displayed age-dependent declines. Expression of the master cell cycle regulators p53 and p21 and growth factors HGF and VEGF also declined significantly at 26 months. These changes were evident despite multiple cell divisions in vitro after bone marrow isolation. CONCLUSIONS: The results suggest that MSCs are subject to molecular genetic changes during aging that are conserved during passage in culture. These changes may affect the physiological functions and the potential of autologous MSCs for stem cell therapy. BioMed Central 2010-04-07 /pmc/articles/PMC2873471/ /pubmed/20374652 http://dx.doi.org/10.1186/1471-2164-11-229 Text en Copyright ©2010 Wilson et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Wilson, Amber
Shehadeh, Lina A
Yu, Hong
Webster, Keith A
Age-related molecular genetic changes of murine bone marrow mesenchymal stem cells
title Age-related molecular genetic changes of murine bone marrow mesenchymal stem cells
title_full Age-related molecular genetic changes of murine bone marrow mesenchymal stem cells
title_fullStr Age-related molecular genetic changes of murine bone marrow mesenchymal stem cells
title_full_unstemmed Age-related molecular genetic changes of murine bone marrow mesenchymal stem cells
title_short Age-related molecular genetic changes of murine bone marrow mesenchymal stem cells
title_sort age-related molecular genetic changes of murine bone marrow mesenchymal stem cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2873471/
https://www.ncbi.nlm.nih.gov/pubmed/20374652
http://dx.doi.org/10.1186/1471-2164-11-229
work_keys_str_mv AT wilsonamber agerelatedmoleculargeneticchangesofmurinebonemarrowmesenchymalstemcells
AT shehadehlinaa agerelatedmoleculargeneticchangesofmurinebonemarrowmesenchymalstemcells
AT yuhong agerelatedmoleculargeneticchangesofmurinebonemarrowmesenchymalstemcells
AT websterkeitha agerelatedmoleculargeneticchangesofmurinebonemarrowmesenchymalstemcells