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Xenon inhibits excitatory but not inhibitory transmission in rat spinal cord dorsal horn neurons

BACKGROUND: The molecular targets for the promising gaseous anaesthetic xenon are still under investigation. Most studies identify N-methyl-D-aspartate (NMDA) receptors as the primary molecular target for xenon, but the role of α-amino-3-hydroxy-5-methyl-4-isoxazole-4-propionic acid (AMPA) receptors...

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Autores principales: Georgiev, Stefan K, Furue, Hidemasa, Baba, Hiroshi, Kohno, Tatsuro
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2873505/
https://www.ncbi.nlm.nih.gov/pubmed/20444263
http://dx.doi.org/10.1186/1744-8069-6-25
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author Georgiev, Stefan K
Furue, Hidemasa
Baba, Hiroshi
Kohno, Tatsuro
author_facet Georgiev, Stefan K
Furue, Hidemasa
Baba, Hiroshi
Kohno, Tatsuro
author_sort Georgiev, Stefan K
collection PubMed
description BACKGROUND: The molecular targets for the promising gaseous anaesthetic xenon are still under investigation. Most studies identify N-methyl-D-aspartate (NMDA) receptors as the primary molecular target for xenon, but the role of α-amino-3-hydroxy-5-methyl-4-isoxazole-4-propionic acid (AMPA) receptors is less clear. In this study we evaluated the effect of xenon on excitatory and inhibitory synaptic transmission in the superficial dorsal horn of the spinal cord using in vitro patch-clamp recordings from rat spinal cord slices. We further evaluated the effects of xenon on innocuous and noxious stimuli using in vivo patch-clamp method. RESULTS: In vitro, xenon decreased the amplitude and area under the curve of currents induced by exogenous NMDA and AMPA and inhibited dorsal root stimulation-evoked excitatory postsynaptic currents. Xenon decreased the amplitude, but not the frequency, of miniature excitatory postsynaptic currents. There was no discernible effect on miniature or evoked inhibitory postsynaptic currents or on the current induced by inhibitory neurotransmitters. In vivo, xenon inhibited responses to tactile and painful stimuli even in the presence of NMDA receptor antagonist. CONCLUSIONS: Xenon inhibits glutamatergic excitatory transmission in the superficial dorsal horn via a postsynaptic mechanism. There is no substantial effect on inhibitory synaptic transmission at the concentration we used. The blunting of excitation in the dorsal horn lamina II neurons could underlie the analgesic effect of xenon.
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spelling pubmed-28735052010-05-20 Xenon inhibits excitatory but not inhibitory transmission in rat spinal cord dorsal horn neurons Georgiev, Stefan K Furue, Hidemasa Baba, Hiroshi Kohno, Tatsuro Mol Pain Research BACKGROUND: The molecular targets for the promising gaseous anaesthetic xenon are still under investigation. Most studies identify N-methyl-D-aspartate (NMDA) receptors as the primary molecular target for xenon, but the role of α-amino-3-hydroxy-5-methyl-4-isoxazole-4-propionic acid (AMPA) receptors is less clear. In this study we evaluated the effect of xenon on excitatory and inhibitory synaptic transmission in the superficial dorsal horn of the spinal cord using in vitro patch-clamp recordings from rat spinal cord slices. We further evaluated the effects of xenon on innocuous and noxious stimuli using in vivo patch-clamp method. RESULTS: In vitro, xenon decreased the amplitude and area under the curve of currents induced by exogenous NMDA and AMPA and inhibited dorsal root stimulation-evoked excitatory postsynaptic currents. Xenon decreased the amplitude, but not the frequency, of miniature excitatory postsynaptic currents. There was no discernible effect on miniature or evoked inhibitory postsynaptic currents or on the current induced by inhibitory neurotransmitters. In vivo, xenon inhibited responses to tactile and painful stimuli even in the presence of NMDA receptor antagonist. CONCLUSIONS: Xenon inhibits glutamatergic excitatory transmission in the superficial dorsal horn via a postsynaptic mechanism. There is no substantial effect on inhibitory synaptic transmission at the concentration we used. The blunting of excitation in the dorsal horn lamina II neurons could underlie the analgesic effect of xenon. BioMed Central 2010-05-05 /pmc/articles/PMC2873505/ /pubmed/20444263 http://dx.doi.org/10.1186/1744-8069-6-25 Text en Copyright ©2010 Georgiev et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Georgiev, Stefan K
Furue, Hidemasa
Baba, Hiroshi
Kohno, Tatsuro
Xenon inhibits excitatory but not inhibitory transmission in rat spinal cord dorsal horn neurons
title Xenon inhibits excitatory but not inhibitory transmission in rat spinal cord dorsal horn neurons
title_full Xenon inhibits excitatory but not inhibitory transmission in rat spinal cord dorsal horn neurons
title_fullStr Xenon inhibits excitatory but not inhibitory transmission in rat spinal cord dorsal horn neurons
title_full_unstemmed Xenon inhibits excitatory but not inhibitory transmission in rat spinal cord dorsal horn neurons
title_short Xenon inhibits excitatory but not inhibitory transmission in rat spinal cord dorsal horn neurons
title_sort xenon inhibits excitatory but not inhibitory transmission in rat spinal cord dorsal horn neurons
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2873505/
https://www.ncbi.nlm.nih.gov/pubmed/20444263
http://dx.doi.org/10.1186/1744-8069-6-25
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