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A rapid method for the generation of uniform acellular bone explants: a technical note

BACKGROUND: Bone graft studies lack standardized controls. We aim to present a quick and reliable method for the intra-operative generation of acellular bone explants. METHODS: Therefore, ovine cancellous bone explants from the iliac crest were prepared and used to test several methods for the induc...

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Autores principales: Jähn, Katharina, Braunstein, Volker, Furlong, Pamela I, Simpson, Angharad E, Richards, R Geoff, Stoddart, Martin J
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2873550/
https://www.ncbi.nlm.nih.gov/pubmed/20459728
http://dx.doi.org/10.1186/1749-799X-5-32
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author Jähn, Katharina
Braunstein, Volker
Furlong, Pamela I
Simpson, Angharad E
Richards, R Geoff
Stoddart, Martin J
author_facet Jähn, Katharina
Braunstein, Volker
Furlong, Pamela I
Simpson, Angharad E
Richards, R Geoff
Stoddart, Martin J
author_sort Jähn, Katharina
collection PubMed
description BACKGROUND: Bone graft studies lack standardized controls. We aim to present a quick and reliable method for the intra-operative generation of acellular bone explants. METHODS: Therefore, ovine cancellous bone explants from the iliac crest were prepared and used to test several methods for the induction of cell death. Over night heat inactivation was used as positive treatment control, methods to be investigated included UV light, or X- ray exposure, incubation in a hypotonic solution (salt-free water) and a short cycle of repeated freezing and thawing. RESULTS: Viability of treated and 2 days cultured bone explants was investigated by lactate dehydrogenase assay. Non-treated cultured control explants maintained around 50% osteocyte viability, while osteocyte survival after the positive treatment control was abolished. The most dramatic loss in cell viability, together with a low standard deviation, was a repeated cycle of freezing and thawing. CONCLUSIONS: To summarize, we present a freeze-thaw method for the creation of acellular bone explants, which is easy to perform, not time-consuming and provides consistent results.
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spelling pubmed-28735502010-05-20 A rapid method for the generation of uniform acellular bone explants: a technical note Jähn, Katharina Braunstein, Volker Furlong, Pamela I Simpson, Angharad E Richards, R Geoff Stoddart, Martin J J Orthop Surg Res Technical Note BACKGROUND: Bone graft studies lack standardized controls. We aim to present a quick and reliable method for the intra-operative generation of acellular bone explants. METHODS: Therefore, ovine cancellous bone explants from the iliac crest were prepared and used to test several methods for the induction of cell death. Over night heat inactivation was used as positive treatment control, methods to be investigated included UV light, or X- ray exposure, incubation in a hypotonic solution (salt-free water) and a short cycle of repeated freezing and thawing. RESULTS: Viability of treated and 2 days cultured bone explants was investigated by lactate dehydrogenase assay. Non-treated cultured control explants maintained around 50% osteocyte viability, while osteocyte survival after the positive treatment control was abolished. The most dramatic loss in cell viability, together with a low standard deviation, was a repeated cycle of freezing and thawing. CONCLUSIONS: To summarize, we present a freeze-thaw method for the creation of acellular bone explants, which is easy to perform, not time-consuming and provides consistent results. BioMed Central 2010-05-10 /pmc/articles/PMC2873550/ /pubmed/20459728 http://dx.doi.org/10.1186/1749-799X-5-32 Text en Copyright ©2010 Jähn et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Technical Note
Jähn, Katharina
Braunstein, Volker
Furlong, Pamela I
Simpson, Angharad E
Richards, R Geoff
Stoddart, Martin J
A rapid method for the generation of uniform acellular bone explants: a technical note
title A rapid method for the generation of uniform acellular bone explants: a technical note
title_full A rapid method for the generation of uniform acellular bone explants: a technical note
title_fullStr A rapid method for the generation of uniform acellular bone explants: a technical note
title_full_unstemmed A rapid method for the generation of uniform acellular bone explants: a technical note
title_short A rapid method for the generation of uniform acellular bone explants: a technical note
title_sort rapid method for the generation of uniform acellular bone explants: a technical note
topic Technical Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2873550/
https://www.ncbi.nlm.nih.gov/pubmed/20459728
http://dx.doi.org/10.1186/1749-799X-5-32
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