Production of specific antibodies against SARS-coronavirus nucleocapsid protein without cross reactivity with human coronaviruses 229E and OC43

Severe acute respiratory syndrome (SARS) is a life-threatening disease for which accurate diagnosis is essential. Although many tools have been developed for the diagnosis of SARS, false-positive reactions in negative sera may occur because of cross-reactivity with other coronaviruses. We have raise...

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Detalles Bibliográficos
Autores principales: Lee, Hyun-Kyoung, Lee, Byoung-Hee, Seok, Seung-Hyeok, Baek, Min-Won, Lee, Hui-Young, Kim, Dong-Jae, Na, Yi-Rang, Noh, Kyoung-Jin, Park, Sung-Hoon, Kumar, Dutta Noton, Kariwa, Hiroaki, Nakauchi, Mina, Heo, Suk-Jin, Park, Jae-Hak
Formato: Texto
Lenguaje:English
Publicado: The Korean Society of Veterinary Science 2010
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2873818/
https://www.ncbi.nlm.nih.gov/pubmed/20458159
http://dx.doi.org/10.4142/jvs.2010.11.2.165
Descripción
Sumario:Severe acute respiratory syndrome (SARS) is a life-threatening disease for which accurate diagnosis is essential. Although many tools have been developed for the diagnosis of SARS, false-positive reactions in negative sera may occur because of cross-reactivity with other coronaviruses. We have raised polyclonal and monoclonal antibodies (Abs) using a recombinant form of the SARS virus nucleocapsid protein. Cross-reactivity of these anti-SARS Abs against human coronavirus (HCoV) 229E and HCoV OC43 were determined by Western blotting. The Abs produced reacted with recombinant SARS virus nucleocapsid protein, but not with HCoV 229E or HCoV OC43.

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