Development and characterization of stable cell lines constitutively expressing the porcine reproductive and respiratory syndrome virus nucleocapsid protein

Despite global efforts to control porcine reproductive and respiratory syndrome virus (PRRSV) infection, the virus continues to cause economic problems in the swine industry worldwide. In this study, we attempted to generate and characterize a panel of stable BHK cell lines that constitutively expre...

Descripción completa

Detalles Bibliográficos
Autores principales: Sagong, Mingeun, Park, Choi-Kyu, Kim, Seong-Hee, Moon, Sung-Up, Cho, Seong-Cheol, Lee, Changhee
Formato: Texto
Lenguaje:English
Publicado: The Korean Society of Veterinary Science 2010
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2873819/
https://www.ncbi.nlm.nih.gov/pubmed/20458160
http://dx.doi.org/10.4142/jvs.2010.11.2.169
_version_ 1782181401246826496
author Sagong, Mingeun
Park, Choi-Kyu
Kim, Seong-Hee
Moon, Sung-Up
Cho, Seong-Cheol
Lee, Changhee
author_facet Sagong, Mingeun
Park, Choi-Kyu
Kim, Seong-Hee
Moon, Sung-Up
Cho, Seong-Cheol
Lee, Changhee
author_sort Sagong, Mingeun
collection PubMed
description Despite global efforts to control porcine reproductive and respiratory syndrome virus (PRRSV) infection, the virus continues to cause economic problems in the swine industry worldwide. In this study, we attempted to generate and characterize a panel of stable BHK cell lines that constitutively express the nucleocapsid (N) protein of type 1 or type 2 PRRSV. The established BHK cell lines were found to react well with N-specific antibodies as well as the hyperimmune serum of pigs raised against each genotype of PRRSV. Taken together, the data implicate a potential usefulness for the newly generated stable cell lines as a diagnostic reagent for PRRSV serology.
format Text
id pubmed-2873819
institution National Center for Biotechnology Information
language English
publishDate 2010
publisher The Korean Society of Veterinary Science
record_format MEDLINE/PubMed
spelling pubmed-28738192010-06-01 Development and characterization of stable cell lines constitutively expressing the porcine reproductive and respiratory syndrome virus nucleocapsid protein Sagong, Mingeun Park, Choi-Kyu Kim, Seong-Hee Moon, Sung-Up Cho, Seong-Cheol Lee, Changhee J Vet Sci Short Communication Despite global efforts to control porcine reproductive and respiratory syndrome virus (PRRSV) infection, the virus continues to cause economic problems in the swine industry worldwide. In this study, we attempted to generate and characterize a panel of stable BHK cell lines that constitutively express the nucleocapsid (N) protein of type 1 or type 2 PRRSV. The established BHK cell lines were found to react well with N-specific antibodies as well as the hyperimmune serum of pigs raised against each genotype of PRRSV. Taken together, the data implicate a potential usefulness for the newly generated stable cell lines as a diagnostic reagent for PRRSV serology. The Korean Society of Veterinary Science 2010-06 2010-05-12 /pmc/articles/PMC2873819/ /pubmed/20458160 http://dx.doi.org/10.4142/jvs.2010.11.2.169 Text en Copyright © 2010 The Korean Society of Veterinary Science https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Sagong, Mingeun
Park, Choi-Kyu
Kim, Seong-Hee
Moon, Sung-Up
Cho, Seong-Cheol
Lee, Changhee
Development and characterization of stable cell lines constitutively expressing the porcine reproductive and respiratory syndrome virus nucleocapsid protein
title Development and characterization of stable cell lines constitutively expressing the porcine reproductive and respiratory syndrome virus nucleocapsid protein
title_full Development and characterization of stable cell lines constitutively expressing the porcine reproductive and respiratory syndrome virus nucleocapsid protein
title_fullStr Development and characterization of stable cell lines constitutively expressing the porcine reproductive and respiratory syndrome virus nucleocapsid protein
title_full_unstemmed Development and characterization of stable cell lines constitutively expressing the porcine reproductive and respiratory syndrome virus nucleocapsid protein
title_short Development and characterization of stable cell lines constitutively expressing the porcine reproductive and respiratory syndrome virus nucleocapsid protein
title_sort development and characterization of stable cell lines constitutively expressing the porcine reproductive and respiratory syndrome virus nucleocapsid protein
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2873819/
https://www.ncbi.nlm.nih.gov/pubmed/20458160
http://dx.doi.org/10.4142/jvs.2010.11.2.169
work_keys_str_mv AT sagongmingeun developmentandcharacterizationofstablecelllinesconstitutivelyexpressingtheporcinereproductiveandrespiratorysyndromevirusnucleocapsidprotein
AT parkchoikyu developmentandcharacterizationofstablecelllinesconstitutivelyexpressingtheporcinereproductiveandrespiratorysyndromevirusnucleocapsidprotein
AT kimseonghee developmentandcharacterizationofstablecelllinesconstitutivelyexpressingtheporcinereproductiveandrespiratorysyndromevirusnucleocapsidprotein
AT moonsungup developmentandcharacterizationofstablecelllinesconstitutivelyexpressingtheporcinereproductiveandrespiratorysyndromevirusnucleocapsidprotein
AT choseongcheol developmentandcharacterizationofstablecelllinesconstitutivelyexpressingtheporcinereproductiveandrespiratorysyndromevirusnucleocapsidprotein
AT leechanghee developmentandcharacterizationofstablecelllinesconstitutivelyexpressingtheporcinereproductiveandrespiratorysyndromevirusnucleocapsidprotein

Ejemplares similares