Bioorthogonal metabolic glycoengineering of human larynx carcinoma (HEp-2) cells targeting sialic acid

Sialic acids are located at the termini of mammalian cell-surface glycostructures, which participate in essential interaction processes including adhesion of pathogens prior to infection and immunogenicity. Here we present the synthesis and bioorthogonal metabolic incorporation of the sialic acid an...

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Autores principales: Homann, Arne, Qamar, Riaz-ul, Serim, Sevnur, Dersch, Petra, Seibel, Jürgen
Formato: Texto
Lenguaje:English
Publicado: Beilstein-Institut 2010
Materias:
Full Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2874417/
https://www.ncbi.nlm.nih.gov/pubmed/20502611
http://dx.doi.org/10.3762/bjoc.6.24
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author Homann, Arne
Qamar, Riaz-ul
Serim, Sevnur
Dersch, Petra
Seibel, Jürgen
author_facet Homann, Arne
Qamar, Riaz-ul
Serim, Sevnur
Dersch, Petra
Seibel, Jürgen
author_sort Homann, Arne
collection PubMed
description Sialic acids are located at the termini of mammalian cell-surface glycostructures, which participate in essential interaction processes including adhesion of pathogens prior to infection and immunogenicity. Here we present the synthesis and bioorthogonal metabolic incorporation of the sialic acid analogue N-(1-oxohex-5-ynyl)neuraminic acid (Neu5Hex) into the cell-surface glycocalyx of a human larynx carcinoma cell line (HEp-2) and its fluorescence labelling by click chemistry.
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spelling pubmed-28744172010-05-25 Bioorthogonal metabolic glycoengineering of human larynx carcinoma (HEp-2) cells targeting sialic acid Homann, Arne Qamar, Riaz-ul Serim, Sevnur Dersch, Petra Seibel, Jürgen Beilstein J Org Chem Full Research Paper Sialic acids are located at the termini of mammalian cell-surface glycostructures, which participate in essential interaction processes including adhesion of pathogens prior to infection and immunogenicity. Here we present the synthesis and bioorthogonal metabolic incorporation of the sialic acid analogue N-(1-oxohex-5-ynyl)neuraminic acid (Neu5Hex) into the cell-surface glycocalyx of a human larynx carcinoma cell line (HEp-2) and its fluorescence labelling by click chemistry. Beilstein-Institut 2010-03-08 /pmc/articles/PMC2874417/ /pubmed/20502611 http://dx.doi.org/10.3762/bjoc.6.24 Text en Copyright © 2010, Homann et al. https://creativecommons.org/licenses/by/2.0https://www.beilstein-journals.org/bjoc/termsThis is an Open Access article under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The license is subject to the Beilstein Journal of Organic Chemistry terms and conditions: (https://www.beilstein-journals.org/bjoc/terms)
spellingShingle Full Research Paper
Homann, Arne
Qamar, Riaz-ul
Serim, Sevnur
Dersch, Petra
Seibel, Jürgen
Bioorthogonal metabolic glycoengineering of human larynx carcinoma (HEp-2) cells targeting sialic acid
title Bioorthogonal metabolic glycoengineering of human larynx carcinoma (HEp-2) cells targeting sialic acid
title_full Bioorthogonal metabolic glycoengineering of human larynx carcinoma (HEp-2) cells targeting sialic acid
title_fullStr Bioorthogonal metabolic glycoengineering of human larynx carcinoma (HEp-2) cells targeting sialic acid
title_full_unstemmed Bioorthogonal metabolic glycoengineering of human larynx carcinoma (HEp-2) cells targeting sialic acid
title_short Bioorthogonal metabolic glycoengineering of human larynx carcinoma (HEp-2) cells targeting sialic acid
title_sort bioorthogonal metabolic glycoengineering of human larynx carcinoma (hep-2) cells targeting sialic acid
topic Full Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2874417/
https://www.ncbi.nlm.nih.gov/pubmed/20502611
http://dx.doi.org/10.3762/bjoc.6.24
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