Cargando…

Protective effect of resin adsorption on septic plasma-induced tubular injury

INTRODUCTION: A pro-apoptotic effect of circulating mediators on renal tubular epithelial cells has been involved in the pathogenesis of sepsis-associated acute kidney injury (AKI). Adsorption techniques have been showed to efficiently remove inflammatory cytokines from plasma. The aim of this study...

Descripción completa

Detalles Bibliográficos
Autores principales: Cantaluppi, Vincenzo, Weber, Viktoria, Lauritano, Carola, Figliolini, Federico, Beltramo, Silvia, Biancone, Luigi, De Cal, Massimo, Cruz, Dinna, Ronco, Claudio, Segoloni, Giuseppe Paolo, Tetta, Ciro, Camussi, Giovanni
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2875506/
https://www.ncbi.nlm.nih.gov/pubmed/20064258
http://dx.doi.org/10.1186/cc8835
_version_ 1782181587944734720
author Cantaluppi, Vincenzo
Weber, Viktoria
Lauritano, Carola
Figliolini, Federico
Beltramo, Silvia
Biancone, Luigi
De Cal, Massimo
Cruz, Dinna
Ronco, Claudio
Segoloni, Giuseppe Paolo
Tetta, Ciro
Camussi, Giovanni
author_facet Cantaluppi, Vincenzo
Weber, Viktoria
Lauritano, Carola
Figliolini, Federico
Beltramo, Silvia
Biancone, Luigi
De Cal, Massimo
Cruz, Dinna
Ronco, Claudio
Segoloni, Giuseppe Paolo
Tetta, Ciro
Camussi, Giovanni
author_sort Cantaluppi, Vincenzo
collection PubMed
description INTRODUCTION: A pro-apoptotic effect of circulating mediators on renal tubular epithelial cells has been involved in the pathogenesis of sepsis-associated acute kidney injury (AKI). Adsorption techniques have been showed to efficiently remove inflammatory cytokines from plasma. The aim of this study was to evaluate the efficiency of the hydrophobic resin Amberchrom CG161 M to adsorb from septic plasma soluble mediators involved in tubular injury. METHODS: We enrolled in the study 10 critically ill patients with sepsis-associated AKI and we evaluated the effects of their plasma on granulocyte adhesion, apoptosis and functional alterations of cultured human kidney tubular epithelial cells. We established an in vitro model of plasma adsorption and we studied the protective effect of unselective removal of soluble mediators by the Amberchrom CG161 M resin on septic plasma-induced tubular cell injury. RESULTS: Plasma from septic patients induced granulocyte adhesion, apoptosis and altered polarity in tubular cells. Plasma adsorption significantly decreased these effects and abated the concentrations of several soluble mediators. The inhibition of granulocyte adhesion to tubular cells was associated with the down-regulation of ICAM-1 and CD40. Resin adsorption inhibited tubular cell apoptosis induced by septic plasma by down-regulating the activation of caspase-3, 8, 9 and of Fas/death receptor-mediated signalling pathways. The alteration of cell polarity, morphogenesis, protein reabsorption and the down-regulation of the tight junction molecule ZO-1, of the sodium transporter NHE3, of the glucose transporter GLUT-2 and of the endocytic receptor megalin all induced by septic plasma were significantly reduced by resin adsorption. CONCLUSIONS: Septic plasma induced a direct injury of tubular cells by favouring granulocyte adhesion, by inducing cell apoptosis and by altering cell polarity and function. All these biological effects are related to the presence of circulating inflammatory mediators that can be efficiently removed by resin adsorption with a consequent limitation of tubular cell injury.
format Text
id pubmed-2875506
institution National Center for Biotechnology Information
language English
publishDate 2010
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-28755062010-05-26 Protective effect of resin adsorption on septic plasma-induced tubular injury Cantaluppi, Vincenzo Weber, Viktoria Lauritano, Carola Figliolini, Federico Beltramo, Silvia Biancone, Luigi De Cal, Massimo Cruz, Dinna Ronco, Claudio Segoloni, Giuseppe Paolo Tetta, Ciro Camussi, Giovanni Crit Care Research INTRODUCTION: A pro-apoptotic effect of circulating mediators on renal tubular epithelial cells has been involved in the pathogenesis of sepsis-associated acute kidney injury (AKI). Adsorption techniques have been showed to efficiently remove inflammatory cytokines from plasma. The aim of this study was to evaluate the efficiency of the hydrophobic resin Amberchrom CG161 M to adsorb from septic plasma soluble mediators involved in tubular injury. METHODS: We enrolled in the study 10 critically ill patients with sepsis-associated AKI and we evaluated the effects of their plasma on granulocyte adhesion, apoptosis and functional alterations of cultured human kidney tubular epithelial cells. We established an in vitro model of plasma adsorption and we studied the protective effect of unselective removal of soluble mediators by the Amberchrom CG161 M resin on septic plasma-induced tubular cell injury. RESULTS: Plasma from septic patients induced granulocyte adhesion, apoptosis and altered polarity in tubular cells. Plasma adsorption significantly decreased these effects and abated the concentrations of several soluble mediators. The inhibition of granulocyte adhesion to tubular cells was associated with the down-regulation of ICAM-1 and CD40. Resin adsorption inhibited tubular cell apoptosis induced by septic plasma by down-regulating the activation of caspase-3, 8, 9 and of Fas/death receptor-mediated signalling pathways. The alteration of cell polarity, morphogenesis, protein reabsorption and the down-regulation of the tight junction molecule ZO-1, of the sodium transporter NHE3, of the glucose transporter GLUT-2 and of the endocytic receptor megalin all induced by septic plasma were significantly reduced by resin adsorption. CONCLUSIONS: Septic plasma induced a direct injury of tubular cells by favouring granulocyte adhesion, by inducing cell apoptosis and by altering cell polarity and function. All these biological effects are related to the presence of circulating inflammatory mediators that can be efficiently removed by resin adsorption with a consequent limitation of tubular cell injury. BioMed Central 2010 2010-01-11 /pmc/articles/PMC2875506/ /pubmed/20064258 http://dx.doi.org/10.1186/cc8835 Text en Copyright ©2010 Cantaluppi et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Cantaluppi, Vincenzo
Weber, Viktoria
Lauritano, Carola
Figliolini, Federico
Beltramo, Silvia
Biancone, Luigi
De Cal, Massimo
Cruz, Dinna
Ronco, Claudio
Segoloni, Giuseppe Paolo
Tetta, Ciro
Camussi, Giovanni
Protective effect of resin adsorption on septic plasma-induced tubular injury
title Protective effect of resin adsorption on septic plasma-induced tubular injury
title_full Protective effect of resin adsorption on septic plasma-induced tubular injury
title_fullStr Protective effect of resin adsorption on septic plasma-induced tubular injury
title_full_unstemmed Protective effect of resin adsorption on septic plasma-induced tubular injury
title_short Protective effect of resin adsorption on septic plasma-induced tubular injury
title_sort protective effect of resin adsorption on septic plasma-induced tubular injury
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2875506/
https://www.ncbi.nlm.nih.gov/pubmed/20064258
http://dx.doi.org/10.1186/cc8835
work_keys_str_mv AT cantaluppivincenzo protectiveeffectofresinadsorptiononsepticplasmainducedtubularinjury
AT weberviktoria protectiveeffectofresinadsorptiononsepticplasmainducedtubularinjury
AT lauritanocarola protectiveeffectofresinadsorptiononsepticplasmainducedtubularinjury
AT figliolinifederico protectiveeffectofresinadsorptiononsepticplasmainducedtubularinjury
AT beltramosilvia protectiveeffectofresinadsorptiononsepticplasmainducedtubularinjury
AT bianconeluigi protectiveeffectofresinadsorptiononsepticplasmainducedtubularinjury
AT decalmassimo protectiveeffectofresinadsorptiononsepticplasmainducedtubularinjury
AT cruzdinna protectiveeffectofresinadsorptiononsepticplasmainducedtubularinjury
AT roncoclaudio protectiveeffectofresinadsorptiononsepticplasmainducedtubularinjury
AT segolonigiuseppepaolo protectiveeffectofresinadsorptiononsepticplasmainducedtubularinjury
AT tettaciro protectiveeffectofresinadsorptiononsepticplasmainducedtubularinjury
AT camussigiovanni protectiveeffectofresinadsorptiononsepticplasmainducedtubularinjury