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CD16 (FcRγIII) as a potential marker of osteoclast precursors in psoriatic arthritis

INTRODUCTION: Psoriatic arthritis (PsA) is a chronic inflammatory arthritis characterized by bone erosion mediated by osteoclasts (OC). Our previous studies showed an elevated frequency of OC precursors (OCP) in PsA patients. Here, we examined if OC arise from CD16-positive monocytes in PsA. METHODS...

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Autores principales: Chiu, Yahui Grace, Shao, Tianmeng, Feng, Changyong, Mensah, Kofi A, Thullen, Michael, Schwarz, Edward M, Ritchlin, Christopher T
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2875642/
https://www.ncbi.nlm.nih.gov/pubmed/20102624
http://dx.doi.org/10.1186/ar2915
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author Chiu, Yahui Grace
Shao, Tianmeng
Feng, Changyong
Mensah, Kofi A
Thullen, Michael
Schwarz, Edward M
Ritchlin, Christopher T
author_facet Chiu, Yahui Grace
Shao, Tianmeng
Feng, Changyong
Mensah, Kofi A
Thullen, Michael
Schwarz, Edward M
Ritchlin, Christopher T
author_sort Chiu, Yahui Grace
collection PubMed
description INTRODUCTION: Psoriatic arthritis (PsA) is a chronic inflammatory arthritis characterized by bone erosion mediated by osteoclasts (OC). Our previous studies showed an elevated frequency of OC precursors (OCP) in PsA patients. Here, we examined if OC arise from CD16-positive monocytes in PsA. METHODS: Peripheral blood mononuclear cells (PBMC) or monocytes were isolated from human peripheral blood and sorted based on CD16 expression. Sorted cells were cultured alone or with bone wafers in the presence of receptor activator of nuclear factor kappa-B ligand (RANKL) and macrophage colony-stimulating factor (M-CSF). Enumeration and bone erosion activity of OC were examined after culture. The effects of tumor necrosis factor-alpha (TNFα), OC-promoting (M-CSF plus RANKL), and dendritic cell (DC)-promoting (GM-CSF plus interleukin (IL)-4) cytokines on CD16 surface expression were examined by flow cytometry. RESULTS: PsA and psoriasis (Ps) subjects had a higher percentage of circulating inflammatory CD14+CD16+ cells than healthy controls (HC). Exposure of cells to OC-promoting, but not DC-promoting media, was associated with CD16 up-regulation. PBMC of Ps and PsA had a higher frequency of cells expressing intermediate levels of CD16. OC were mainly derived from CD16+ cells in PsA. Increased CD16 expression was associated with a higher bone erosion activity in PsA. CONCLUSIONS: An increased frequency of circulating CD14+CD16+ cells was noted in PsA compared to controls, and intermediate levels of CD16 may suggest a transitional state of OCP during osteoclastogenesis. Intriguingly, TNFα blocked CD16 expression on a subset of CD14+ monocytes. Collectively, our data suggest that CD16 has the potential to serve as an OCP marker in inflammatory arthritis.
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spelling pubmed-28756422010-05-26 CD16 (FcRγIII) as a potential marker of osteoclast precursors in psoriatic arthritis Chiu, Yahui Grace Shao, Tianmeng Feng, Changyong Mensah, Kofi A Thullen, Michael Schwarz, Edward M Ritchlin, Christopher T Arthritis Res Ther Research article INTRODUCTION: Psoriatic arthritis (PsA) is a chronic inflammatory arthritis characterized by bone erosion mediated by osteoclasts (OC). Our previous studies showed an elevated frequency of OC precursors (OCP) in PsA patients. Here, we examined if OC arise from CD16-positive monocytes in PsA. METHODS: Peripheral blood mononuclear cells (PBMC) or monocytes were isolated from human peripheral blood and sorted based on CD16 expression. Sorted cells were cultured alone or with bone wafers in the presence of receptor activator of nuclear factor kappa-B ligand (RANKL) and macrophage colony-stimulating factor (M-CSF). Enumeration and bone erosion activity of OC were examined after culture. The effects of tumor necrosis factor-alpha (TNFα), OC-promoting (M-CSF plus RANKL), and dendritic cell (DC)-promoting (GM-CSF plus interleukin (IL)-4) cytokines on CD16 surface expression were examined by flow cytometry. RESULTS: PsA and psoriasis (Ps) subjects had a higher percentage of circulating inflammatory CD14+CD16+ cells than healthy controls (HC). Exposure of cells to OC-promoting, but not DC-promoting media, was associated with CD16 up-regulation. PBMC of Ps and PsA had a higher frequency of cells expressing intermediate levels of CD16. OC were mainly derived from CD16+ cells in PsA. Increased CD16 expression was associated with a higher bone erosion activity in PsA. CONCLUSIONS: An increased frequency of circulating CD14+CD16+ cells was noted in PsA compared to controls, and intermediate levels of CD16 may suggest a transitional state of OCP during osteoclastogenesis. Intriguingly, TNFα blocked CD16 expression on a subset of CD14+ monocytes. Collectively, our data suggest that CD16 has the potential to serve as an OCP marker in inflammatory arthritis. BioMed Central 2010 2010-01-26 /pmc/articles/PMC2875642/ /pubmed/20102624 http://dx.doi.org/10.1186/ar2915 Text en Copyright ©2010 Chiu et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research article
Chiu, Yahui Grace
Shao, Tianmeng
Feng, Changyong
Mensah, Kofi A
Thullen, Michael
Schwarz, Edward M
Ritchlin, Christopher T
CD16 (FcRγIII) as a potential marker of osteoclast precursors in psoriatic arthritis
title CD16 (FcRγIII) as a potential marker of osteoclast precursors in psoriatic arthritis
title_full CD16 (FcRγIII) as a potential marker of osteoclast precursors in psoriatic arthritis
title_fullStr CD16 (FcRγIII) as a potential marker of osteoclast precursors in psoriatic arthritis
title_full_unstemmed CD16 (FcRγIII) as a potential marker of osteoclast precursors in psoriatic arthritis
title_short CD16 (FcRγIII) as a potential marker of osteoclast precursors in psoriatic arthritis
title_sort cd16 (fcrγiii) as a potential marker of osteoclast precursors in psoriatic arthritis
topic Research article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2875642/
https://www.ncbi.nlm.nih.gov/pubmed/20102624
http://dx.doi.org/10.1186/ar2915
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