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S100b Counteracts Neurodegeneration of Rat Cholinergic Neurons in Brain Slices after Oxygen-Glucose Deprivation

Alzheimer's disease is a severe chronic neurodegenerative disorder characterized by beta-amyloid plaques, tau pathology, cerebrovascular damage, inflammation, reactive gliosis, and cell death of cholinergic neurons. The aim of the present study is to test whether the glia-derived molecule S100b...

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Autores principales: Serbinek, Daniela, Ullrich, Celine, Pirchl, Michael, Hochstrasser, Tanja, Schmidt-Kastner, Rainald, Humpel, Christian
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2875695/
https://www.ncbi.nlm.nih.gov/pubmed/20508809
http://dx.doi.org/10.1155/2010/106123
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author Serbinek, Daniela
Ullrich, Celine
Pirchl, Michael
Hochstrasser, Tanja
Schmidt-Kastner, Rainald
Humpel, Christian
author_facet Serbinek, Daniela
Ullrich, Celine
Pirchl, Michael
Hochstrasser, Tanja
Schmidt-Kastner, Rainald
Humpel, Christian
author_sort Serbinek, Daniela
collection PubMed
description Alzheimer's disease is a severe chronic neurodegenerative disorder characterized by beta-amyloid plaques, tau pathology, cerebrovascular damage, inflammation, reactive gliosis, and cell death of cholinergic neurons. The aim of the present study is to test whether the glia-derived molecule S100b can counteract neurodegeneration of cholinergic neurons after oxygen-glucose deprivation (OGD) in organotypic brain slices of basal nucleus of Meynert. Our data showed that 3 days of OGD induced a marked decrease of cholinergic neurons (60% of control), which could be counteracted by 50 μg/mL recombinant S100b. The effect was dose and time dependent. Application of nerve growth factor or fibroblast growth factor-2 was less protective. C-fos-like immunoreactivity was enhanced 3 hours after OGD indicating metabolic stress. We conclude that S100b is a potent neuroprotective factor for cholinergic neurons during ischemic events.
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spelling pubmed-28756952010-05-27 S100b Counteracts Neurodegeneration of Rat Cholinergic Neurons in Brain Slices after Oxygen-Glucose Deprivation Serbinek, Daniela Ullrich, Celine Pirchl, Michael Hochstrasser, Tanja Schmidt-Kastner, Rainald Humpel, Christian Cardiovasc Psychiatry Neurol Research Article Alzheimer's disease is a severe chronic neurodegenerative disorder characterized by beta-amyloid plaques, tau pathology, cerebrovascular damage, inflammation, reactive gliosis, and cell death of cholinergic neurons. The aim of the present study is to test whether the glia-derived molecule S100b can counteract neurodegeneration of cholinergic neurons after oxygen-glucose deprivation (OGD) in organotypic brain slices of basal nucleus of Meynert. Our data showed that 3 days of OGD induced a marked decrease of cholinergic neurons (60% of control), which could be counteracted by 50 μg/mL recombinant S100b. The effect was dose and time dependent. Application of nerve growth factor or fibroblast growth factor-2 was less protective. C-fos-like immunoreactivity was enhanced 3 hours after OGD indicating metabolic stress. We conclude that S100b is a potent neuroprotective factor for cholinergic neurons during ischemic events. Hindawi Publishing Corporation 2010 2010-05-24 /pmc/articles/PMC2875695/ /pubmed/20508809 http://dx.doi.org/10.1155/2010/106123 Text en Copyright © 2010 Daniela Serbinek et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Serbinek, Daniela
Ullrich, Celine
Pirchl, Michael
Hochstrasser, Tanja
Schmidt-Kastner, Rainald
Humpel, Christian
S100b Counteracts Neurodegeneration of Rat Cholinergic Neurons in Brain Slices after Oxygen-Glucose Deprivation
title S100b Counteracts Neurodegeneration of Rat Cholinergic Neurons in Brain Slices after Oxygen-Glucose Deprivation
title_full S100b Counteracts Neurodegeneration of Rat Cholinergic Neurons in Brain Slices after Oxygen-Glucose Deprivation
title_fullStr S100b Counteracts Neurodegeneration of Rat Cholinergic Neurons in Brain Slices after Oxygen-Glucose Deprivation
title_full_unstemmed S100b Counteracts Neurodegeneration of Rat Cholinergic Neurons in Brain Slices after Oxygen-Glucose Deprivation
title_short S100b Counteracts Neurodegeneration of Rat Cholinergic Neurons in Brain Slices after Oxygen-Glucose Deprivation
title_sort s100b counteracts neurodegeneration of rat cholinergic neurons in brain slices after oxygen-glucose deprivation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2875695/
https://www.ncbi.nlm.nih.gov/pubmed/20508809
http://dx.doi.org/10.1155/2010/106123
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