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S100b Counteracts Neurodegeneration of Rat Cholinergic Neurons in Brain Slices after Oxygen-Glucose Deprivation
Alzheimer's disease is a severe chronic neurodegenerative disorder characterized by beta-amyloid plaques, tau pathology, cerebrovascular damage, inflammation, reactive gliosis, and cell death of cholinergic neurons. The aim of the present study is to test whether the glia-derived molecule S100b...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Hindawi Publishing Corporation
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2875695/ https://www.ncbi.nlm.nih.gov/pubmed/20508809 http://dx.doi.org/10.1155/2010/106123 |
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author | Serbinek, Daniela Ullrich, Celine Pirchl, Michael Hochstrasser, Tanja Schmidt-Kastner, Rainald Humpel, Christian |
author_facet | Serbinek, Daniela Ullrich, Celine Pirchl, Michael Hochstrasser, Tanja Schmidt-Kastner, Rainald Humpel, Christian |
author_sort | Serbinek, Daniela |
collection | PubMed |
description | Alzheimer's disease is a severe chronic neurodegenerative disorder characterized by beta-amyloid plaques, tau pathology, cerebrovascular damage, inflammation, reactive gliosis, and cell death of cholinergic neurons. The aim of the present study is to test whether the glia-derived molecule S100b can counteract neurodegeneration of cholinergic neurons after oxygen-glucose deprivation (OGD) in organotypic brain slices of basal nucleus of Meynert. Our data showed that 3 days of OGD induced a marked decrease of cholinergic neurons (60% of control), which could be counteracted by 50 μg/mL recombinant S100b. The effect was dose and time dependent. Application of nerve growth factor or fibroblast growth factor-2 was less protective. C-fos-like immunoreactivity was enhanced 3 hours after OGD indicating metabolic stress. We conclude that S100b is a potent neuroprotective factor for cholinergic neurons during ischemic events. |
format | Text |
id | pubmed-2875695 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-28756952010-05-27 S100b Counteracts Neurodegeneration of Rat Cholinergic Neurons in Brain Slices after Oxygen-Glucose Deprivation Serbinek, Daniela Ullrich, Celine Pirchl, Michael Hochstrasser, Tanja Schmidt-Kastner, Rainald Humpel, Christian Cardiovasc Psychiatry Neurol Research Article Alzheimer's disease is a severe chronic neurodegenerative disorder characterized by beta-amyloid plaques, tau pathology, cerebrovascular damage, inflammation, reactive gliosis, and cell death of cholinergic neurons. The aim of the present study is to test whether the glia-derived molecule S100b can counteract neurodegeneration of cholinergic neurons after oxygen-glucose deprivation (OGD) in organotypic brain slices of basal nucleus of Meynert. Our data showed that 3 days of OGD induced a marked decrease of cholinergic neurons (60% of control), which could be counteracted by 50 μg/mL recombinant S100b. The effect was dose and time dependent. Application of nerve growth factor or fibroblast growth factor-2 was less protective. C-fos-like immunoreactivity was enhanced 3 hours after OGD indicating metabolic stress. We conclude that S100b is a potent neuroprotective factor for cholinergic neurons during ischemic events. Hindawi Publishing Corporation 2010 2010-05-24 /pmc/articles/PMC2875695/ /pubmed/20508809 http://dx.doi.org/10.1155/2010/106123 Text en Copyright © 2010 Daniela Serbinek et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Serbinek, Daniela Ullrich, Celine Pirchl, Michael Hochstrasser, Tanja Schmidt-Kastner, Rainald Humpel, Christian S100b Counteracts Neurodegeneration of Rat Cholinergic Neurons in Brain Slices after Oxygen-Glucose Deprivation |
title | S100b Counteracts Neurodegeneration of Rat Cholinergic Neurons in Brain Slices after Oxygen-Glucose Deprivation |
title_full | S100b Counteracts Neurodegeneration of Rat Cholinergic Neurons in Brain Slices after Oxygen-Glucose Deprivation |
title_fullStr | S100b Counteracts Neurodegeneration of Rat Cholinergic Neurons in Brain Slices after Oxygen-Glucose Deprivation |
title_full_unstemmed | S100b Counteracts Neurodegeneration of Rat Cholinergic Neurons in Brain Slices after Oxygen-Glucose Deprivation |
title_short | S100b Counteracts Neurodegeneration of Rat Cholinergic Neurons in Brain Slices after Oxygen-Glucose Deprivation |
title_sort | s100b counteracts neurodegeneration of rat cholinergic neurons in brain slices after oxygen-glucose deprivation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2875695/ https://www.ncbi.nlm.nih.gov/pubmed/20508809 http://dx.doi.org/10.1155/2010/106123 |
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