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Stc1: A Critical Link between RNAi and Chromatin Modification Required for Heterochromatin Integrity

In fission yeast, RNAi directs heterochromatin formation at centromeres, telomeres, and the mating type locus. Noncoding RNAs transcribed from repeat elements generate siRNAs that are incorporated into the Argonaute-containing RITS complex and direct it to nascent homologous transcripts. This leads...

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Autores principales: Bayne, Elizabeth H., White, Sharon A., Kagansky, Alexander, Bijos, Dominika A., Sanchez-Pulido, Luis, Hoe, Kwang-Lae, Kim, Dong-Uk, Park, Han-Oh, Ponting, Chris P., Rappsilber, Juri, Allshire, Robin C.
Formato: Texto
Lenguaje:English
Publicado: Cell Press 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2875855/
https://www.ncbi.nlm.nih.gov/pubmed/20211136
http://dx.doi.org/10.1016/j.cell.2010.01.038
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author Bayne, Elizabeth H.
White, Sharon A.
Kagansky, Alexander
Bijos, Dominika A.
Sanchez-Pulido, Luis
Hoe, Kwang-Lae
Kim, Dong-Uk
Park, Han-Oh
Ponting, Chris P.
Rappsilber, Juri
Allshire, Robin C.
author_facet Bayne, Elizabeth H.
White, Sharon A.
Kagansky, Alexander
Bijos, Dominika A.
Sanchez-Pulido, Luis
Hoe, Kwang-Lae
Kim, Dong-Uk
Park, Han-Oh
Ponting, Chris P.
Rappsilber, Juri
Allshire, Robin C.
author_sort Bayne, Elizabeth H.
collection PubMed
description In fission yeast, RNAi directs heterochromatin formation at centromeres, telomeres, and the mating type locus. Noncoding RNAs transcribed from repeat elements generate siRNAs that are incorporated into the Argonaute-containing RITS complex and direct it to nascent homologous transcripts. This leads to recruitment of the CLRC complex, including the histone methyltransferase Clr4, promoting H3K9 methylation and heterochromatin formation. A key question is what mediates the recruitment of Clr4/CLRC to transcript-bound RITS. We have identified a LIM domain protein, Stc1, that is required for centromeric heterochromatin integrity. Our analyses show that Stc1 is specifically required to establish H3K9 methylation via RNAi, and interacts both with the RNAi effector Ago1, and with the chromatin-modifying CLRC complex. Moreover, tethering Stc1 to a euchromatic locus is sufficient to induce silencing and heterochromatin formation independently of RNAi. We conclude that Stc1 associates with RITS on centromeric transcripts and recruits CLRC, thereby coupling RNAi to chromatin modification.
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spelling pubmed-28758552010-06-10 Stc1: A Critical Link between RNAi and Chromatin Modification Required for Heterochromatin Integrity Bayne, Elizabeth H. White, Sharon A. Kagansky, Alexander Bijos, Dominika A. Sanchez-Pulido, Luis Hoe, Kwang-Lae Kim, Dong-Uk Park, Han-Oh Ponting, Chris P. Rappsilber, Juri Allshire, Robin C. Cell Article In fission yeast, RNAi directs heterochromatin formation at centromeres, telomeres, and the mating type locus. Noncoding RNAs transcribed from repeat elements generate siRNAs that are incorporated into the Argonaute-containing RITS complex and direct it to nascent homologous transcripts. This leads to recruitment of the CLRC complex, including the histone methyltransferase Clr4, promoting H3K9 methylation and heterochromatin formation. A key question is what mediates the recruitment of Clr4/CLRC to transcript-bound RITS. We have identified a LIM domain protein, Stc1, that is required for centromeric heterochromatin integrity. Our analyses show that Stc1 is specifically required to establish H3K9 methylation via RNAi, and interacts both with the RNAi effector Ago1, and with the chromatin-modifying CLRC complex. Moreover, tethering Stc1 to a euchromatic locus is sufficient to induce silencing and heterochromatin formation independently of RNAi. We conclude that Stc1 associates with RITS on centromeric transcripts and recruits CLRC, thereby coupling RNAi to chromatin modification. Cell Press 2010-03-05 /pmc/articles/PMC2875855/ /pubmed/20211136 http://dx.doi.org/10.1016/j.cell.2010.01.038 Text en © 2010 ELL & Excerpta Medica. https://creativecommons.org/licenses/by/3.0/ Open Access under CC BY 3.0 (https://creativecommons.org/licenses/by/3.0/) license
spellingShingle Article
Bayne, Elizabeth H.
White, Sharon A.
Kagansky, Alexander
Bijos, Dominika A.
Sanchez-Pulido, Luis
Hoe, Kwang-Lae
Kim, Dong-Uk
Park, Han-Oh
Ponting, Chris P.
Rappsilber, Juri
Allshire, Robin C.
Stc1: A Critical Link between RNAi and Chromatin Modification Required for Heterochromatin Integrity
title Stc1: A Critical Link between RNAi and Chromatin Modification Required for Heterochromatin Integrity
title_full Stc1: A Critical Link between RNAi and Chromatin Modification Required for Heterochromatin Integrity
title_fullStr Stc1: A Critical Link between RNAi and Chromatin Modification Required for Heterochromatin Integrity
title_full_unstemmed Stc1: A Critical Link between RNAi and Chromatin Modification Required for Heterochromatin Integrity
title_short Stc1: A Critical Link between RNAi and Chromatin Modification Required for Heterochromatin Integrity
title_sort stc1: a critical link between rnai and chromatin modification required for heterochromatin integrity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2875855/
https://www.ncbi.nlm.nih.gov/pubmed/20211136
http://dx.doi.org/10.1016/j.cell.2010.01.038
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