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Stc1: A Critical Link between RNAi and Chromatin Modification Required for Heterochromatin Integrity
In fission yeast, RNAi directs heterochromatin formation at centromeres, telomeres, and the mating type locus. Noncoding RNAs transcribed from repeat elements generate siRNAs that are incorporated into the Argonaute-containing RITS complex and direct it to nascent homologous transcripts. This leads...
Autores principales: | , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Cell Press
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2875855/ https://www.ncbi.nlm.nih.gov/pubmed/20211136 http://dx.doi.org/10.1016/j.cell.2010.01.038 |
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author | Bayne, Elizabeth H. White, Sharon A. Kagansky, Alexander Bijos, Dominika A. Sanchez-Pulido, Luis Hoe, Kwang-Lae Kim, Dong-Uk Park, Han-Oh Ponting, Chris P. Rappsilber, Juri Allshire, Robin C. |
author_facet | Bayne, Elizabeth H. White, Sharon A. Kagansky, Alexander Bijos, Dominika A. Sanchez-Pulido, Luis Hoe, Kwang-Lae Kim, Dong-Uk Park, Han-Oh Ponting, Chris P. Rappsilber, Juri Allshire, Robin C. |
author_sort | Bayne, Elizabeth H. |
collection | PubMed |
description | In fission yeast, RNAi directs heterochromatin formation at centromeres, telomeres, and the mating type locus. Noncoding RNAs transcribed from repeat elements generate siRNAs that are incorporated into the Argonaute-containing RITS complex and direct it to nascent homologous transcripts. This leads to recruitment of the CLRC complex, including the histone methyltransferase Clr4, promoting H3K9 methylation and heterochromatin formation. A key question is what mediates the recruitment of Clr4/CLRC to transcript-bound RITS. We have identified a LIM domain protein, Stc1, that is required for centromeric heterochromatin integrity. Our analyses show that Stc1 is specifically required to establish H3K9 methylation via RNAi, and interacts both with the RNAi effector Ago1, and with the chromatin-modifying CLRC complex. Moreover, tethering Stc1 to a euchromatic locus is sufficient to induce silencing and heterochromatin formation independently of RNAi. We conclude that Stc1 associates with RITS on centromeric transcripts and recruits CLRC, thereby coupling RNAi to chromatin modification. |
format | Text |
id | pubmed-2875855 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Cell Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-28758552010-06-10 Stc1: A Critical Link between RNAi and Chromatin Modification Required for Heterochromatin Integrity Bayne, Elizabeth H. White, Sharon A. Kagansky, Alexander Bijos, Dominika A. Sanchez-Pulido, Luis Hoe, Kwang-Lae Kim, Dong-Uk Park, Han-Oh Ponting, Chris P. Rappsilber, Juri Allshire, Robin C. Cell Article In fission yeast, RNAi directs heterochromatin formation at centromeres, telomeres, and the mating type locus. Noncoding RNAs transcribed from repeat elements generate siRNAs that are incorporated into the Argonaute-containing RITS complex and direct it to nascent homologous transcripts. This leads to recruitment of the CLRC complex, including the histone methyltransferase Clr4, promoting H3K9 methylation and heterochromatin formation. A key question is what mediates the recruitment of Clr4/CLRC to transcript-bound RITS. We have identified a LIM domain protein, Stc1, that is required for centromeric heterochromatin integrity. Our analyses show that Stc1 is specifically required to establish H3K9 methylation via RNAi, and interacts both with the RNAi effector Ago1, and with the chromatin-modifying CLRC complex. Moreover, tethering Stc1 to a euchromatic locus is sufficient to induce silencing and heterochromatin formation independently of RNAi. We conclude that Stc1 associates with RITS on centromeric transcripts and recruits CLRC, thereby coupling RNAi to chromatin modification. Cell Press 2010-03-05 /pmc/articles/PMC2875855/ /pubmed/20211136 http://dx.doi.org/10.1016/j.cell.2010.01.038 Text en © 2010 ELL & Excerpta Medica. https://creativecommons.org/licenses/by/3.0/ Open Access under CC BY 3.0 (https://creativecommons.org/licenses/by/3.0/) license |
spellingShingle | Article Bayne, Elizabeth H. White, Sharon A. Kagansky, Alexander Bijos, Dominika A. Sanchez-Pulido, Luis Hoe, Kwang-Lae Kim, Dong-Uk Park, Han-Oh Ponting, Chris P. Rappsilber, Juri Allshire, Robin C. Stc1: A Critical Link between RNAi and Chromatin Modification Required for Heterochromatin Integrity |
title | Stc1: A Critical Link between RNAi and Chromatin Modification Required for Heterochromatin Integrity |
title_full | Stc1: A Critical Link between RNAi and Chromatin Modification Required for Heterochromatin Integrity |
title_fullStr | Stc1: A Critical Link between RNAi and Chromatin Modification Required for Heterochromatin Integrity |
title_full_unstemmed | Stc1: A Critical Link between RNAi and Chromatin Modification Required for Heterochromatin Integrity |
title_short | Stc1: A Critical Link between RNAi and Chromatin Modification Required for Heterochromatin Integrity |
title_sort | stc1: a critical link between rnai and chromatin modification required for heterochromatin integrity |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2875855/ https://www.ncbi.nlm.nih.gov/pubmed/20211136 http://dx.doi.org/10.1016/j.cell.2010.01.038 |
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